(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date 27 January 2011 (27.01.2011) WO 2011/011767 Al (51) International Patent Classification: 12/842,991 23 July 2010 (23.07.2010) US C12N 15/87 (2006.01) 12/842,999 23 July 2010 (23.07.2010) US 12/843,000 23 July 2010 (23.07.2010) US (21) International Application Number: PCT/US20 10/043 167 (71) Applicant (for all designated States except US): SIGMA- ALDRICH CO. [US/US]; 3050 Spruce Street, St. Louis, (22) International Filing Date: Missouri 63 103 (US). 23 July 20 10 (23 .07.2010) (72) Inventors; and (25) Filing Language: English (75) Inventors/Applicants (for US only): WEINSTEIN, Ed¬ (26) Publication Language: English ward [US/US]; 3050 Spruce Street, St. Louis, Missouri 63 103 (US). CUI, Xiaoxia [US/US]; 3050 Spruce Street, (30) Priority Data: St. Louis, Missouri 63103 (US). SIMMONS, Phil 61/228,4 19 24 July 2009 (24.07.2009) US [US/US]; 3050 Spruce Street, St. Louis, Missouri 63 103 61/232,620 10 August 2009 (10.08.2009) US (US). 61/245,877 25 September 2009 (25.09.2009) us 61/263,696 23 November 2009 (23.1 1.2009) us (74) Agents: DOTY, Kathryn et al; Polsinelli Shughart PC, 61/263,904 24 November 2009 (24.1 1.2009) us Mark Twain Plaza III, 105 West Vandalia, Suite 400, Ed- 61/336,000 14 January 2010 (14.01 .2010) us wardsville, IIL 62025 (US). 61/308,089 25 February 2010 (25.02.2010) us (81) Designated States (unless otherwise indicated, for every 61/309,729 2 March 2010 (02.03.2010) us kind of national protection available): AE, AG, AL, AM, 61/323,702 13 April 2010 (13.04.2010) us AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, 61/323,698 13 April 2010 (13.04.2010) us CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, 61/323,7 19 13 April 2010 (13.04.2010) us DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, 61/343,287 26 April 2010 (26.04.2010) us HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, 12/842,2 17 23 July 2010 (23.07.2010) us KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, 12/842,2 19 23 July 2010 (23.07.2010) us ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, 12/842,269 23 July 2010 (23.07.2010) us NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, 12/842,208 23 July 2010 (23.07.2010) us SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, 12/842,204 23 July 2010 (23.07.2010) us TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. 12/842,1 98 23 July 2010 (23.07.2010) us 12/842,1 88 23 July 2010 (23.07.2010) us (84) Designated States (unless otherwise indicated, for every 12/842,7 19 23 July 2010 (23.07.2010) us kind of regional protection available): ARIPO (BW, GH, 12/842,7 13 23 July 2010 (23.07.2010) us GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, 12/842,708 23 July 2010 (23.07.2010) us ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ, 12/842,694 23 July 2010 (23.07.2010) us TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, 12/842,678 23 July 2010 (23.07.2010) us EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, 12/842,666 23 July 2010 (23.07.2010) us LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, 12/842,620 23 July 2010 (23.07.2010) us SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, 12/842,578 23 July 2010 (23.07.2010) us GW, ML, MR, NE, SN, TD, TG). 12/842,897 23 July 2010 (23.07.2010) us 12/842,893 23 July 2010 (23.07.2010) us Published: 12/842,886 23 July 2010 (23.07.2010) us — with international search report (Art. 21(3)) 12/842,839 23 July 2010 (23.07.2010) us 12/842,980 23 July 2010 (23.07.2010) us — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of 12/842,978 23 July 2010 (23.07.2010) us 12/842,976 23 July 2010 (23.07.2010) us amendments (Rule 48.2(h)) 12/842,982 23 July 2010 (23.07.2010) us — with sequence listing part of description (Rule 5.2(a)) 12/842,994 23 July 2010 (23.07.2010) us 12/842,993 23 July 2010 (23.07.2010) us (54) Title: METHOD FOR GENOME EDITING (57) Abstract: The present invention encompasses a method for creating an animal or cell with at least one chromosomal edit. In particular, the invention relates to the use of targeted zinc finger nucleases to edit chromosomal sequences. The invention further encompasses an animal or a cell created by a method of the invention. METHOD FOR GENOME EDITING REFERENCE TO SEQUENCE LISTING [0001 ] A paper copy of the sequence listing and a computer readable form of the same sequence listing are appended below and herein incorporated by reference. The information recorded in computer readable form is identical to the written sequence listing, according to 37 C .F.R . 1.821 (f). FIELD OF THE INVENTION [0002] The invention encompasses a method for creating an animal or cell with at least one chromosomal edit. In particular, the invention relates to the use of targeted zinc finger nucleases to edit chromosomal sequences. BACKGROUND OF THE INVENTION [0003] Rational genome engineering has enormous potential across basic research, drug discovery, and cell-based medicines. Existing methods for targeted gene knock-out or site-specific gene insertion rely on homologous recombination. The low rate of spontaneous recombination in certain cell types, however, has been an enormous hurdle to universal genome editing. The scale of screening effort and the time required to isolate the targeted event was prohibitive. Thus, there exists a strong need for a technology that can rapidly achieve genomic editing in most cell types with high speed and efficiency, so as to greatly reduce the overall engineering effort. SUMMARY OF THE INVENTION [0004] One aspect of the present invention encompasses a method for editing a chromosomal sequence. The method comprises, in part, (a) introducing into a cell comprising the chromosomal sequence at least one nucleic acid encoding a zinc finger nuclease that recognizes a target sequence in the chromosomal sequence and is able to cleave a cleavage site in the chromosomal sequence, and, optionally, (i) at least one donor polynucleotide comprising a donor sequence for integration, an upstream sequence, and a downstream sequence, wherein the donor sequence is flanked by the upstream sequence and the downstream sequence, and wherein the upstream sequence and the downstream sequence share substantial sequence identity with either side of the cleavage site, or (ii) at least one exchange polynucleotide comprising an exchange sequence that is substantially identical to a portion of the chromosomal sequence at the cleavage site, and further comprising at least one nucleotide change; and (b) cultuhng the cell to allow expression of the zinc finger nuclease such that the zinc finger nuclease introduces a double-stranded break into the chromosomal sequence at the cleavage site, and wherein the double-stranded break is repaired by (i) a non-homologous end-joining repair process such that a mutation is introduced into the chromosomal sequence, or optionally (ii) a homology-directed repair process such that the donor sequence is integrated into the chromosomal sequence or the exchange sequence is exchanged with the portion of the chromosomal sequence. [0005] Another aspect of the present invention encompasses a non- human animal. The non-human animal may be created in part, by (a) introducing into a cell comprising the chromosomal sequence at least one nucleic acid encoding a zinc finger nuclease that recognizes a target sequence in the chromosomal sequence and is able to cleave a cleavage site in the chromosomal sequence, and, optionally, (i) at least one donor polynucleotide comprising a donor sequence for integration, an upstream sequence, and a downstream sequence, wherein the donor sequence is flanked by the upstream sequence and the downstream sequence, and wherein the upstream sequence and the downstream sequence share substantial sequence identity with either side of the cleavage site, or (ii) at least one exchange polynucleotide comprising an exchange sequence that is substantially identical to a portion of the chromosomal sequence at the cleavage site, and further comprising at least one nucleotide change; and (b) cultuhng the cell to allow expression of the zinc finger nuclease such that the zinc finger nuclease introduces a double-stranded break into the chromosomal sequence at the cleavage site, and wherein the double-stranded break is repaired by (i) a non-homologous end-joining repair process such that a mutation is introduced into the chromosomal sequence, or optionally (ii) a homology-directed repair process such that the donor sequence is integrated into the chromosomal sequence or the exchange sequence is exchanged with the portion of the chromosomal sequence. [0006] Yet another aspect of the present invention encompasses a cell. The cell may be created in part, by in part, by (a) introducing into the cell comprising the chromosomal sequence at least one nucleic acid encoding a zinc finger nuclease that recognizes a target sequence in the chromosomal sequence and is able to cleave a cleavage site in the chromosomal sequence, and, optionally, (i) at least one donor polynucleotide comprising a donor sequence for integration, an upstream sequence, and a downstream sequence, wherein the donor sequence is flanked by the upstream sequence and the downstream sequence, and wherein the upstream sequence and the downstream sequence share substantial sequence identity with either side of the cleavage site, or (ii) at least one exchange polynucleotide comprising