Zoosyst. Evol. 97 (2) 2021, 383–392 | DOI 10.3897/zse.97.69089 Three new species of the spider genus Nopsma (Araneae, Caponiidae, Nopinae) from Colombia Alexander Sánchez-Ruiz1, Leonel Martínez2, Alexandre B. Bonaldo1 1 Museu Paraense Emílio Goeldi, Coordenação de Zoologia, Laboratório de Aracnologia. Av. Perimetral, 1901, Terra Firme, CEP 66077–830, Belém, Pará, Brazil 2 Grupo de Investigación Biodiversidad del Caribe Colombiano, Semillero de Investigación Sistemática de Artrópodos Neotropicales, Departamento de Biología, Universidad del Atlántico, Barranquilla, Colombia http://zoobank.org/0D9C19DB-BBED-4F03-A691-AFBA7AA9195E Corresponding author: Alexander Sánchez-Ruiz ([email protected]) Academic editor: Danilo Harms ♦ Received 24 May 2021 ♦ Accepted 21 June 2021 ♦ Published 13 July 2021 Abstract Three new Colombian species of the spider genus Nopsma Sánchez-Ruiz, Brescovit & Bonaldo, 2020 are described and illustrat- ed: Nopsma leticia sp. nov. (male) from Amazonas department, Nopsma macagual sp. nov. (male) from Caquetá department and Nopsma paya sp. nov. (male and female) from Putumayo department. The collection data of the holotype of Nopsma florencia Sánchez-Ruiz, Brescovit & Bonaldo are corrected. Additionally, an updated identification key for all species of the genus and a dis- tribution map for the Colombian species are included. Key Words Arachnida, neotropical region, synspermiata, taxonomy Introduction graphed prior to the publication by the second author of this paper (L.M.), who unfortunately confused the type´s The two-eyed spider genus Nopsma was recently pro- data label with those of other Colombian Nopsma spec- posed by Sánchez-Ruiz et al. (2020) for four species oc- imens from Macagual in Caquetá department, while the curring in Colombia, Ecuador, Peru and Nicaragua. The actual locality of N. florencia is in Chocó department. The Ecuadorian species Nopsma juchuy (Dupérré, 2014) was collection data of this Colombian species is corrected be- transferred from Nyetnops Platnick & Lise, 2007 and low. On the bright side, this mislabeling event led to the was elected as the type species. Members of this genus discovery of a new Nopsma species, since the specimens can be distinguished from other two-eyed Nopinae (ex- from Macagual belong to an undescribed species. This cept Nyetnops), by lacking a crista and an arolium on finding also triggered the opportunity to review caponiids anterior metatarsi, and from Nyetnops by the presence from three Colombian collections, leading to the discov- of a gladius. Additionally, Nopsma species are charac- ery of two additional undescribed Nopsma species. These terized by having an elongated, prolaterally protruded three species are herein described, along with the correc- embolus, unique among nopines, and by the shape of tion of the type locality of N. florencia. Photos of the hab- endites, without projected outer sides on anterior margin itus, male palpal morphology and female genital organs, (Sánchez-Ruiz et al. 2020). line drawings of the male copulatory bulbs and schematic Members of Nopsma are poorly known in Colom- drawings of the female internal genitalia are provided. bia, being represented by only one species, N. florencia Additionally, a distribution map for all Colombian spe- Sánchez-Ruiz, Brescovit & Bonaldo, 2020. The species cies and an updated identification key for all species of was described from male specimens revised and photo- the genus are presented. Copyright Alexander Sánchez-Ruiz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 384 Sánchez-Ruiz, A. et al.: Three new Colombian species of Nopsma Materials and methods at the proportion of 1 tablet/5 ml. After cleaning, samples were immersed in clove oil for visualization of internal The specimens examined in this study were supplied by structures. The terminology for copulatory structures fol- the following collections (acronym and curator in pa- lows Sánchez-Ruiz et al. (2015). All digital photos were rentheses): Instituto Alexander Von Humboldt, Bogotá, edited using Adobe Photoshop CS ver. 12.0 and the dis- Colombia (IAvH-I, J.C. Neita) Instituto de Ciencias Na- tribution map was prepared in QGIS (QGIS 2021). Plates turales of the Universidad Nacional, Bogotá, Colombia were edited with Corel Draw X7 ver. 17.1. Geographic (ICN-Ar, E. Flórez) and Pontificia Universidad Javeriana, coordinates were extracted from original labels. Locality Bogotá (MPUJ-ENT, D. Forero). elevations refer to meters above sea level. Morphological observations and illustrations were The following abbreviations are used in the text and fig- made using a Leica MC125 stereomicroscope with a cam- ures: ap = anterior plate, as = anterior tracheal spiracles, dmr era lucida. Multifocal images were taken with the Leica = distal margin of receptaculum, e = embolus, ess = external MC-190 HD and Leica MC-170 HD digital cameras at- sclerotization around spiracles, go = genital opening (go- tached to Leica S8AP0 and Leica MC125 stereomicro- nopore), mk = membranous keel on embolus, re = recepta- scopes respectively with extended focal range. All mul- culun, pmr = proximal margin of receptaculum, pp = pos- tifocal images were assembled using Helicon Focus Pro terior plate, ps = posterior tracheal spiracles, t = tegulum. ver. 5.3.14. The measurements are in millimeters (mm) and were made using an ocular micrometer. Descriptions and measurements follow Sánchez-Ruiz and Bresco- Taxonomy vit (2018). Coloration patterns were described based on specimens preserved in 70–80% ethanol. The internal fe- Caponiidae Simon, 1890 male genitalia was dissected with fine forceps and their soft tissues were digested for 24 hours with Ultrazyme Genus Nopsma Sánchez-Ruiz, Brescovit & enzymatic eye lens cleaner, diluted with distilled water Bonaldo, 2020 Updated key to the species of Nopsma (males only) 1 Large tegulum, reaching or exceeding the palpal tibia length (Sánchez-Ruiz et al. 2020: figs 11B, 17B) ...................... 2 – Small tegulum, not reaching the palpal tibia length (Sánchez-Ruiz et al. 2020: figs 15B, 18B) .................................... 3 2 Elongated palpal tibia, two times the patella length (Sánchez-Ruiz et al. 2020: fig. 11B), embolus projecting from the prolateral distal surface of the tegulum with a keel bordering the tip (Sánchez-Ruiz et al. 2020: figs 11B, C, 14A, D–F) ..................................................................................................................................................N. enriquei – Short palpal tibia, just a little longer than patella length (Sánchez-Ruiz et al. 2020: fig. 17B), embolus projecting from the prolateral median surface of the tegulum with three very thin, long projections on the tip (Sánchez-Ruiz et al. 2020: fig. 17B, C, F) .............................................................................................................................................N. armandoi 3 Embolus posteriorly directed (Figs 1C, D, 2C, D, 4E, H) .............................................................................................. 4 – Embolus anteriorly directed (Fig. 3C, D) ........................................................................................N. macagual sp. nov. 4 Tegulum one-third of the cymbium length (Figs 2C, D, 3C, D, 4E, F) ........................................................................... 5 – Tegulum conspicuously small, only one-fifth the cymbium length (Fig. 1C, D) .............................................. N. florencia 5 Embolus with a membranous keel at the opening, extended proximally towards the embolus shaft (Fig. 6E, F) ........... 6 – Embolus with membranous keel restricted to the opening of embolus tip (Fig. 6G, H) ........................... N. paya sp. nov. 6 Membranous keel long, reaching more than one-third of the embolus shaft (Fig. 6F) ........................... N. leticia sp. nov. – Membranous keel short, reaching only one-fourth or less of the embolus shaft (Sánchez-Ruiz et al. 2020: fig. 16B–E) ... ........................................................................................................................................................................N. juchuy Nopsma florenciaSánchez-Ruiz, Brescovit & Bonaldo, 2020 Remark. The type locality of this species is here cor- Figures 1A–D, 5A, B, 6A, B, 8 rected. The data labels of the holotype and paratype re- ported in the original description are actually those be- Nopsma florencia Sánchez-Ruiz, Brescovit & Bonaldo, 2020: 483, longing to Nopsma macagual sp. nov. fig. 18A–F. Diagnosis. Males of Nopsma florencia resemble those of Nopsma leticia sp. nov. by the similarly shaped mem- branous keel on embolus tip (Fig. 6A, B, E, F), but can Type material. Holotype ♂, COLOMBIA: Chocó de- be distinguished by the conspicuous small oval tegulum, partment, Jardín Botánico El Darién, Capurgana, Acan- with only one-fifth the cymbium length (Fig. 1C, D), dí, Camino a los Ríos; 8°37'53.95"N, 77°21'23.43"W; (one-third in N. leticia sp. nov., Fig. 2C, D) and by the 260 m; 14 April 2008; C. Peña leg; pitfall trap; MPUJ- enlarged embolus (Fig. 1C, D) (shorter in N. leticia sp. ENT 61986; examined, type locality corrected. nov., Fig. 2C, D). zse.pensoft.net Zoosyst. Evol. 97 (2) 2021, 383–392 385 Figure 1. Nopsma florencia Sánchez-Ruiz, Brescovit & Bonaldo, male