Dataset S1 Effects of proteasome inhibition and deletion of Bag domain on association of proteins with Bag3 Full Length DeltaC Full Length BAG3 + BAG3 + Proteins BAG3 MG MG BAG family molecular chaperone regulator 3 OS=Homo sapiens GN=BAG3 PE=1 SV=3 BAG3_HUMAN Chaperone Heat shock cognate 71 kDa protein OS=Homo sapiens GN=HSPA8 PE=1 SV=1 HSP7C_HUMAN Chaperone 1.0000 0.4664 0.1197 Non-POU domain-containing octamer-binding protein OS=Homo sapiens GN=NONO PE=1 SV=4 NONO_HUMAN 1.0000 1.4531 2.2689 Heat shock 70 kDa protein 1A/1B OS=Homo sapiens GN=HSPA1A PE=1 SV=5 HSP71_HUMAN Chaperone 1.0000 0.3953 0.0917 Actin cytoplasmic 2 OS=Homo sapiens GN=ACTG1 PE=1 SV=1 ACTG_HUMAN Cytoskeleton 1.0000 0.0999 1.0625 Actin cytoplasmic 1 OS=Homo sapiens GN=ACTB PE=1 SV=1 ACTB_HUMAN Cytoskeleton 1.0000 0.3875 0.6755 Splicing factor proline- and glutamine-rich OS=Homo sapiens GN=SFPQ PE=1 SV=2 SFPQ_HUMAN 1.0000 1.7130 1.1645 Pyruvate kinase PKM OS=Homo sapiens GN=PKM PE=1 SV=4 KPYM_HUMAN 1.0000 0.1069 0.5828 Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 SV=4 EF2_HUMAN 1.0000 0.5441 0.5029 78 kDa glucose-regulated protein OS=Homo sapiens GN=HSPA5 PE=1 SV=2 GRP78_HUMAN Chaperone 1.0000 0.8638 0.6490 Tubulin beta chain OS=Homo sapiens GN=TUBB PE=1 SV=2 TBB5_HUMAN Cytoskeleton 1.0000 0.0978 0.6262 Erythrocyte band 7 integral membrane protein OS=Homo sapiens GN=STOM PE=1 SV=3 STOM_HUMAN 1.0000 1.2862 1.1878 Heat shock protein HSP 90-beta OS=Homo sapiens GN=HSP90AB1 PE=1 SV=4 HS90B_HUMAN Chaperone 1.0000 0.6000 0.6635 Alpha-enolase OS=Homo sapiens GN=ENO1 PE=1 SV=2 ENOA_HUMAN 1.0000 0.7151 1.0212 Elongation factor 1-alpha 1 OS=Homo sapiens GN=EEF1A1 PE=1 SV=1 EF1A1_HUMAN Chaperone 1.0000 0.0745 0.5675 Peroxiredoxin-1 OS=Homo sapiens GN=PRDX1 PE=1 SV=1 PRDX1_HUMAN 1.0000 0.3115 0.1408 Tubulin alpha-1C chain OS=Homo sapiens GN=TUBA1C PE=1 SV=1 TBA1C_HUMAN Cytoskeleton 1.0000 0.0000 0.4706 Heat shock protein beta-1 OS=Homo sapiens GN=HSPB1 PE=1 SV=2 HSPB1_HUMAN Chaperone 1.0000 0.2781 0.9153 Filamin-A OS=Homo sapiens GN=FLNA PE=1 SV=4 FLNA_HUMAN Cytoskeleton 1.0000 0.5938 0.3081 Polypeptide N-acetylgalactosaminyltransferase 2 OS=Homo sapiens GN=GALNT2 PE=1 SV=1 GALT2_HUMAN 1.0000 0.7273 0.8656 Signaling Fatty acid synthase OS=Homo sapiens GN=FASN PE=1 SV=3 FAS_HUMAN factor 1.0000 0.6576 0.6377 Peptidyl-prolyl cis-trans isomerase A OS=Homo sapiens GN=PPIA PE=1 SV=2 PPIA_HUMAN Chaperone 1.0000 0.3311 0.2079 T-complex protein 1 subunit eta OS=Homo sapiens GN=CCT7 PE=1 SV=2 TCPH_HUMAN Chaperone 1.0000 0.3984 0.6412 Fructose-bisphosphate aldolase A OS=Homo sapiens GN=ALDOA PE=1 SV=2 ALDOA_HUMAN 1.0000 0.1459 0.5689 Tubulin alpha-4A chain OS=Homo sapiens GN=TUBA4A PE=1 SV=1 TBA4A_HUMAN Cytoskeleton 1.0000 0.0000 0.5413 T-complex protein 1 subunit gamma OS=Homo sapiens GN=CCT3 PE=1 SV=4 TCPG_HUMAN Chaperone 1.0000 0.4057 0.5679 Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 OS=Homo sapiens GN=DHX15 PE=1 SV=2 DHX15_HUMAN 1.0000 1.7784 2.3078 ATP-citrate synthase OS=Homo sapiens GN=ACLY PE=1 SV=3 ACLY_HUMAN 1.0000 0.8348 0.6814 Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3 G3P_HUMAN 1.0000 0.1115 0.3549 T-complex protein 1 subunit delta OS=Homo sapiens GN=CCT4 PE=1 SV=4 TCPD_HUMAN Chaperone 1.0000 0.4871 0.4782 Zinc transporter 1 OS=Homo sapiens GN=SLC30A1 PE=1 SV=3 ZNT1_HUMAN 1.0000 0.6117 0.9919 Signaling Annexin A2 OS=Homo sapiens GN=ANXA2 PE=1 SV=2 ANXA2_HUMAN factor 1.0000 0.6616 0.2696 T-complex protein 1 subunit beta OS=Homo sapiens GN=CCT2 PE=1 SV=4 TCPB_HUMAN Chaperone 1.0000 0.3762 0.6676 Heat shock protein HSP 90-alpha OS=Homo sapiens GN=HSP90AA1 PE=1 SV=5 HS90A_HUMAN Chaperone 1.0000 0.5468 0.6360 Granulins OS=Homo sapiens GN=GRN PE=1 SV=2 GRN_HUMAN 1.0000 1.7027 1.0674 Poly(rC)-binding protein 1 OS=Homo sapiens GN=PCBP1 PE=1 SV=2 PCBP1_HUMAN 1.0000 0.6749 0.8201 www.pnas.org/cgi/doi/10.1073/pnas.1803130115 60 kDa heat shock protein mitochondrial OS=Homo sapiens GN=HSPD1 PE=1 SV=2 CH60_HUMAN 1.0000 0.2085 0.6890 Signaling Epidermal growth factor receptor OS=Homo sapiens GN=EGFR PE=1 SV=2 EGFR_HUMAN factor 1.0000 1.2282 1.4705 Multifunctional protein ADE2 OS=Homo sapiens GN=PAICS PE=1 SV=3 PUR6_HUMAN 1.0000 0.7524 0.6634 DNA topoisomerase 1 OS=Homo sapiens GN=TOP1 PE=1 SV=2 TOP1_HUMAN 1.0000 1.2734 1.8084 Signaling Ezrin OS=Homo sapiens GN=EZR PE=1 SV=4 EZRI_HUMAN factor 1.0000 1.0663 0.6927 Phosphoglycerate kinase 1 OS=Homo sapiens GN=PGK1 PE=1 SV=3 PGK1_HUMAN 1.0000 0.2756 0.4271 T-complex protein 1 subunit epsilon OS=Homo sapiens GN=CCT5 PE=1 SV=1 TCPE_HUMAN Chaperone 1.0000 0.6582 0.2986 LIM and SH3 domain protein 1 OS=Homo sapiens GN=LASP1 PE=1 SV=2 LASP1_HUMAN Cytoskeleton 1.0000 0.6821 0.4911 Stress-induced-phosphoprotein 1 OS=Homo sapiens GN=STIP1 PE=1 SV=1 STIP1_HUMAN Chaperone 1.0000 0.7210 0.2436 ATP-dependent RNA helicase DDX42 OS=Homo sapiens GN=DDX42 PE=1 SV=1 DDX42_HUMAN 1.0000 0.6004 0.8034 T-complex protein 1 subunit theta OS=Homo sapiens GN=CCT8 PE=1 SV=4 TCPQ_HUMAN Chaperone 1.0000 0.6258 0.7152 Transcriptional repressor protein YY1 OS=Homo sapiens GN=YY1 PE=1 SV=2 TYY1_HUMAN 1.0000 0.7863 0.8012 Cofilin-1 OS=Homo sapiens GN=CFL1 PE=1 SV=3 COF1_HUMAN Cytoskeleton 1.0000 0.5098 0.8934 Eukaryotic translation initiation factor 5A-1 OS=Homo sapiens GN=EIF5A PE=1 SV=2 IF5A1_HUMAN 1.0000 0.2174 0.5094 Zinc transporter ZIP10 OS=Homo sapiens GN=SLC39A10 PE=1 SV=2 S39AA_HUMAN 1.0000 1.3921 0.8672 Signaling 14-3-3 protein zeta/delta OS=Homo sapiens GN=YWHAZ PE=1 SV=1 1433Z_HUMAN factor 1.0000 1.7280 0.0000 Protein disulfide-isomerase A3 OS=Homo sapiens GN=PDIA3 PE=1 SV=4 PDIA3_HUMAN Chaperone 1.0000 0.5623 0.8861 Peroxiredoxin-4 OS=Homo sapiens GN=PRDX4 PE=1 SV=1 PRDX4_HUMAN 1.0000 0.9211 0.7464 D-3-phosphoglycerate dehydrogenase OS=Homo sapiens GN=PHGDH PE=1 SV=4 SERA_HUMAN Chaperone 1.0000 0.7443 0.2308 Alpha-actinin-4 OS=Homo sapiens GN=ACTN4 PE=1 SV=2 ACTN4_HUMAN Cytoskeleton 1.0000 0.3823 0.4333 Ubiquitin-60S ribosomal protein L40 OS=Homo sapiens GN=UBA52 PE=1 SV=2 RL40_HUMAN 1.0000 2.8518 1.5032 T-complex protein 1 subunit zeta OS=Homo sapiens GN=CCT6A PE=1 SV=3 TCPZ_HUMAN Chaperone 1.0000 0.4986 0.2180 Signaling 14-3-3 protein beta/alpha OS=Homo sapiens GN=YWHAB PE=1 SV=3 1433B_HUMAN factor 1.0000 1.3300 0.8016 Pleckstrin homology-like domain family A member 1 OS=Homo sapiens Signaling GN=PHLDA1 PE=1 SV=4 PHLA1_HUMAN factor 1.0000 1.0475 0.3494 Elongation factor Tu mitochondrial OS=Homo sapiens GN=TUFM PE=1 SV=2 EFTU_HUMAN 1.0000 0.7367 0.3485 Serum albumin OS=Homo sapiens GN=ALB PE=1 SV=2 ALBU_HUMAN 1.0000 1.0342 0.5854 Signaling 14-3-3 protein gamma OS=Homo sapiens GN=YWHAG PE=1 SV=2 1433G_HUMAN factor 1.0000 1.2109 0.9017 Eukaryotic initiation factor 4A-I OS=Homo sapiens GN=EIF4A1 PE=1 SV=1 IF4A1_HUMAN 1.0000 0.6714 0.1160 ATP synthase subunit beta mitochondrial OS=Homo sapiens GN=ATP5B PE=1 SV=3 ATPB_HUMAN 1.0000 0.1462 0.5469 Transitional endoplasmic reticulum ATPase OS=Homo sapiens GN=VCP PE=1 Signaling SV=4 TERA_HUMAN factor 1.0000 0.4483 0.4224 ATP synthase subunit alpha mitochondrial OS=Homo sapiens GN=ATP5A1 PE=1 SV=1 ATPA_HUMAN 1.0000 0.3860 0.6225 Testin OS=Homo sapiens GN=TES PE=1 SV=1 TES_HUMAN 1.0000 0.8547 0.4226 Signaling Coatomer subunit alpha OS=Homo sapiens GN=COPA PE=1 SV=2 COPA_HUMAN factor 1.0000 0.3876 0.2336 Peroxiredoxin-2 OS=Homo sapiens GN=PRDX2 PE=1 SV=5 PRDX2_HUMAN 1.0000 0.5131 0.1684 Heat shock protein beta-8 OS=Homo sapiens GN=HSPB8 PE=1 SV=1 HSPB8_HUMAN Chaperone 1.0000 1.0031 0.9252 L-lactate dehydrogenase B chain OS=Homo sapiens GN=LDHB PE=1 SV=2 LDHB_HUMAN 1.0000 0.9582 0.9447 Signaling WD repeat-containing protein 1 OS=Homo sapiens GN=WDR1 PE=1 SV=4 WDR1_HUMAN factor 1.0000 0.5805 0.2870 Profilin-1 OS=Homo sapiens GN=PFN1 PE=1 SV=2 PROF1_HUMAN Cytoskeleton 1.0000 0.3641 0.7875 L-lactate dehydrogenase A chain OS=Homo sapiens GN=LDHA PE=1 SV=2 LDHA_HUMAN 1.0000 0.6627 0.6650 Transketolase OS=Homo sapiens GN=TKT PE=1 SV=3 TKT_HUMAN 1.0000 0.6148 0.6042 Inosine-5'-monophosphate dehydrogenase 2 OS=Homo sapiens GN=IMPDH2 PE=1 SV=2 IMDH2_HUMAN 1.0000 0.6024 0.0000 Vinculin OS=Homo sapiens GN=VCL PE=1 SV=4 VINC_HUMAN Cytoskeleton 1.0000 0.4515 0.4447 Eukaryotic translation initiation factor 2 subunit 3 OS=Homo sapiens GN=EIF2S3 PE=1 SV=3 IF2G_HUMAN 1.0000 0.6862 0.6477 Heterogeneous nuclear ribonucleoprotein L OS=Homo sapiens GN=HNRNPL PE=1 SV=2 HNRPL_HUMAN 1.0000 2.5729 2.1460 Threonine--tRNA ligase cytoplasmic OS=Homo sapiens GN=TARS PE=1 SV=3 SYTC_HUMAN 1.0000 0.0000 0.4872 Stress-70 protein mitochondrial OS=Homo sapiens GN=HSPA9 PE=1 SV=2 GRP75_HUMAN Chaperone 1.0000 1.0541 0.6863 Glutathione S-transferase P OS=Homo sapiens GN=GSTP1 PE=1 SV=2 GSTP1_HUMAN 1.0000 0.3250 0.6600 Putative nucleoside diphosphate kinase OS=Homo sapiens GN=NME2P1 PE=5 SV=1 NDK8_HUMAN 1.0000 0.6464 0.4020 Signaling Cytoskeleton-associated protein 4 OS=Homo sapiens GN=CKAP4 PE=1 SV=2 CKAP4_HUMAN factor 1.0000 0.8313 2.3207 Activated RNA polymerase II transcriptional coactivator p15 OS=Homo sapiens GN=SUB1 PE=1 SV=3 TCP4_HUMAN Chaperone 1.0000 0.8486 0.4508 Splicing factor 3B subunit 2 OS=Homo sapiens GN=SF3B2 PE=1 SV=2 SF3B2_HUMAN 1.0000 1.4806 0.3532 Fascin OS=Homo sapiens GN=FSCN1 PE=1 SV=3 FSCN1_HUMAN Cytoskeleton 1.0000 0.6730 0.5491 Signaling Nucleophosmin OS=Homo sapiens GN=NPM1 PE=1 SV=2 NPM_HUMAN factor 1.0000 0.0027 1.0522 Prohibitin OS=Homo sapiens GN=PHB PE=1 SV=1 PHB_HUMAN 1.0000 0.2221 1.8892 C-1-tetrahydrofolate synthase cytoplasmic OS=Homo sapiens GN=MTHFD1 PE=1 SV=3 C1TC_HUMAN 1.0000 0.7847 0.0000 Elongation factor 1-delta OS=Homo sapiens GN=EEF1D PE=1