CKMT2 Antibody Cat. No.: 29-585 CKMT2 Antibody Antibody used in IHC on Human Skeletal muscle at 4.0-8.0 ug/ml. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Dog, Human, Mouse Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human CKMT2. TESTED APPLICATIONS: ELISA, IHC, WB CKMT2 antibody can be used for detection of CKMT2 by ELISA at 1:312500. CKMT2 APPLICATIONS: antibody can be used for detection of CKMT2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate PREDICTED MOLECULAR 46 kDa WEIGHT: September 27, 2021 1 https://www.prosci-inc.com/ckmt2-antibody-29-585.html Properties PURIFICATION: Antibody is purified by protein A chromatography method. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose. CONCENTRATION: batch dependent For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: CKMT2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. Additional Info OFFICIAL SYMBOL: CKMT2 ALTERNATE NAMES: CKMT2, SMTCK ACCESSION NO.: NP_001816 PROTEIN GI NO.: 153251982 GENE ID: 1160 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase.Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria BACKGROUND: to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. REFERENCES: 1) Guerrero, K., Am. J. Physiol. Regul. Integr. Comp. Physiol. 289 (4), R1144-R1154. September 27, 2021 2 https://www.prosci-inc.com/ckmt2-antibody-29-585.html ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 27, 2021 3 https://www.prosci-inc.com/ckmt2-antibody-29-585.html.