Supplementary Information Table S1. The characteristics of known AMPs derived from milk proteins. Boman Net Isoelectric Molecular Instability Aliphatic Sequence AMP Origin/Position GRAVY Half Life Index Charge Point (pH) Mass (Da) Index Index (kcal/mol) Mamalian 1 h RPKHPIK f(1–7) of αS1-casein 3.1 11.17 875.0 −2.029 19.84 55.71 Yeast 2 min 3.68 E.coli 2 min Mamalian 1 h RPKHPIKHQGLPQEVL Isracidin f(1–23) of α -casein 2.2 9.99 2764.2 −0.987 46.49 97.39 Yeast 2 min 2.42 NENLLRF S1 E.coli 2 min Mamalian 10 h GLPQE f(10–14) of αS1-casein −1.0 4.00 542.5 −1.040 123.56 78.00 Yeast >20 h 1.29 E.coli >20 h Mamalian 20 h IKHQGLPQE f(6–14) of αS1-casein 0.1 6.75 1049.1 −1.256 63.66 86.67 Yeast 30 min 1.92 E.coli >10 h Mamalian 100 h VLNENLLR f(15–22) of αS1-casein 0.0 5.97 970.1 0.075 32.83 182.5 Yeast >20 h 2.02 E.coli >10 h Mamalian 5.5 h LRLKKYKVPQL αS1-casein, gen. var. B-8P f(99–109) 4 10.46 1385.7 −0.636 46.18 132.73 Yeast 3 min 1.67 E.coli 2 min Mamalian 1.9 h SDIPNPIGSENSEK f(180–193) of αS1-casein −2 4.14 1486.5 −1.314 32.69 55.71 Yeast >20 h 2.89 E.coli >10 h KTKLTEEEKNRLNFLK Mamalian 1.3 h casocidin-I f(150–188) of α -casein gen. KISQRYQKFALPQYLK S2 7.1 10.08 4869.6 −1.323 27.85 70.00 Yeast 3 min 2.83 var. A TVYQHQK E.coli 2 min Mamalian 100 h VYQHQKAMKPWIQPK f(183–206) α -casein gen. var. A 5.1 10.12 3002.6 −0.783 2.25 72.92 Yeast >20 h 1.37 TKVIPYVRY S2 E.coli >10 h Mamalian 5.5 h LKKISQ f(165–170) of ovine αS2-casein 2.0 10.00 715.8 −0.633 12.13 130.00 Yeast 3 min 1.7 E.coli 2 min Mamalian 100 h VYQHQKAMKPWIQPK f(183–207) of bovine α -casein 5.1 10.12 3115.7 −0.600 2.56 85.60 Yeast >20 h 1.12 TKVIPYVRYL S2 E.coli >10 h S2 Table S1. Cont. Boman Net Isoelectric Molecular Instability Aliphatic Sequence AMP Origin/Position GRAVY Half Life Index Charge Point (pH) Mass (Da) Index Index (kcal/mol) Mamalian 5.5 h LKKISQRYQKFALPQY f(164–179) of bovine αS2-casein 4.0 10.17 2011.4 −0.938 25.45 79.38 Yeast 3 min 2.02 E.coli 2 min Mamalian >20 h PYVRYL f(203–208) of ovine αS2-casein 1.0 9.00 809.9 −0.117 −4.23 113.33 Yeast >20 h 1.04 E.coli ? Mamalian 1.3 h KTVYQHQKAMKPWIQ f(181–207) of ovine α -casein 6.1 10.22 3345.0 −0.726 3.11 79.26 Yeast 3 min 1.33 PKTKVIPYVRYL S2 E.coli 3 min Mamalian 5.5 LKTVYQHQKAMKPWI f(180–207) of ovine α -casein 6.1 10.22 3458.22 −0.564 0.32 90.36 Yeast 3min 1.11 QPKTKVIPYVRYL S2 E.coli 3min ALPQYLKTVYQHQKA Mamalian 4.4 h MKPWIQPKTKVIPYVR f(175–207) of ovine αS2-casein 6.1 10.1 4030.8 −0.503 11.18 91.52 Yeast >20 min 0.91 YL E.coli >10 min QKFALPQYLKTVYQHQ Mamalian 0.8 h KAMKPWIQPKTKVIPY f(172–207) of ovine αS2-casein 7.1 10.17 4434.3 −0.589 11.08 83.89 Yeast 10 min 1.06 VRYL E.coli 10 h LKKISQRYQKFALPQY Mamalian 5.5 h LKTVYQHQKAMKPWI f(164–207) of ovine αS2-casein 10.1 10.35 5451.6 −0.700 9.69 86.36 Yeast 2 min 1.44 QPKTKVIPYVRYL E.coli 2 min Mamalian 5.5 h LKKISQRYQKFALPQ f(164–180) of ovine α -casein 4.0 10.17 2124.5 −0.659 24.54 97.65 Yeast 2 min 1.61 YL S2 E.coli 2 min Mamalian 2.8 h YQEPVLGPVRGPFPI β-casein, gen. var. A2-5P f(193–207) 0 6.00 1668.9 −0.033 80.71 90.67 Yeast 10 min 0.3 E.coli 2 min Mamalian 2.8 h YQEPVLGPVRGPFPIIV β-casein, genetic variant A2-5P f(193–209) 0 6.00 1881.2 0.482 67.4 120.00 Yeast 10 min −0.25 E.coli 2 min MAIPPKKNQDKTEIPTI Mamalian 30 h NTIASGEPTSTPTTEAV Kappacin f(107–169) of κ-casein gen. Yeast 20 h −7.0 4.04 6707.4 −0.370 64.17 77.66 1.55 ESTVATLEDSPEVIESPP var. A E.coli 10 h EINTVQVTSTAV S3 Table S1. Cont. Boman Net Isoelectric Molecular Instability Aliphatic Sequence AMP Origin/Position GRAVY Half Life Index Charge Point (pH) Mass (Da) Index Index (kcal/mol) Mamalian 2.8 h YYQQKPVA f(42–49) of bovine κ-casein 1.0 8.50 996.1 −1.137 62.9 48.75 Yeast 10 min 1.38 E.coli 2 min Mamalian 100 h VQVTSTAV f(162–169) of bovine κ-casein 0.0 5.49 803.9 1.087 −11.29 121.25 Yeast >20 h 0.01 E.coli >10 h Mamalian 1.9 h STVATL f(141–146) of bovine κ-casein 0.0 5.24 590.6 1.267 8.33 130.00 Yeast >20 h −0.37 E.coli >10 h Mamalian 1.1 h FSDKIAK f(18–24) of bovine κ-casein 1.0 8.59 807.9 −0.429 −15.69 70.00 Yeast 3 min 1.93 E.coli 2 min Mamalian 20 h IQY f(28–30) of bovine κ-casein 0.0 6.09 422.79 −0.100 −18.47 130.00 Yeast >30 min 0.25 E.coli >10 h Mamalian 20 h YVL f(30–32) of bovine κ-casein 0.0 6.09 393.51 2.233 6.67 226.67 Yeast 30 min −2.59 E.coli >10 h Mamalian 1 h EIPT f(118–121) of bovine κ-casein −1.0 3.85 458.54 −0.325 3.85 97.50 Yeast 30 min 1.11 E.coli >10 h Mamalian 100 h VESTVATL f(139–146) of bovine κ-casein −1.0 4.00 818.9 1.038 32.83 133.75 Yeast >20 h 0.08 E.coli >10 h Mamalian >20 h PAAVRSPAQILQ f(64–75) of bovine κ-casein 1.0 10.18 1250.4 0.200 157.72 114.17 Yeast >20 h 0.84 E.coli ? Mamalian 4.4 h AVESTVATLEDSPEVIE f(136–156) of bovine κ-casein −6.0 3.40 2199.3 −0.243 134.70 88.10 Yeast >20 h 1.54 SPPE E.coli >10 h Mamalian 1h LTD1-f(1–5) of α-laktoalbumin, gen. EQLTK 0.0 6.10 617.7 −1.560 46.52 78.00 Yeast 30 min 3.11 var. B E.coli >10 h S4 Table S1. Cont. Boman Net Isoelectric Molecular Instability Aliphatic Sequence AMP Origin/Position GRAVY Half Life Index Charge Point (pH) Mass (Da) Index Index (kcal/mol) Mamalian >30 h LDT 2, α-chain f(17–31) of GYGGVSLPEWVCTTF −1.0 4.00 1615.8 0.453 44.95 64.67 Yeast >20 h −0.46 α- laktoalbumin, gen. var. B E.coli >10 h Mamalian 4.4 h LDT 2, β-chain f(109–114) of ALCSEK 0.0 6.04 649.7 −0.017 40.43 81.67 Yeast >20 h 1.29 α-laktoalbumin, . gen. varB E.coli >10 h Mamalian 1.2 h LDC, α-chain f(61–68) of CKDDQNPH −1.0 5.21 956.0 −2.525 5.15 0.00 Yeast >20 h 4.81 α-laktoalbumin, gen. var. B E.coli >10 h Mamalian 20 h LDC, β-chain chain f(75–80) of ISCDKF 0.0 5.83 711.8 0.267 80.62 65.00 Yeast 30 min 1.41 α-laktoalbumin, gen. var. B E.coli >10 h Mamalian 100 h VAGTWY f(15–20) β-laktoglobulin, gen. var. A 0.0 5.49 695.7 0.450 −30.87 65.00 Yeast >20 h −1.06 E.coli >10 h Mamalian 20 h IPAVFK f(78–83) β-laktoglobulin, gen. var. A 1.0 8.75 673.8 1.300 10.58 130.00 Yeast 30 min −1.36 E.coli >10 h Mamalian 4.4 h AASDISLLDAQSAPLR f(25–40) β-laktoglobulin, gen. var. A −1.0 4.21 1627.8 0.256 60.91 122.50 Yeast >20 h 1.32 E.coli >10 h Mamalian 100 h VLVLDTDYK f(92–100) β-laktoglobulin, gen.var. A −1.0 4.21 1065.2 0.344 −7.81 151.11 Yeast >20 h 0.86 E.coli >10 h Mamalian 1.1 h FKCRRWQWRMKKLG bovine lactoferricin B f(17–41) 7.9 11.84 3125.8 −0.576 77.92 50.80 Yeast 3 min 2.75 APSITCVRRAF of lactoferrin E.coli 2 min Mamalian 4.4 h APRKNVRW f(1–8) of lactoferrin 3.0 12.01 1026.2 −1.613 95.00 48.75 Yeast >20 h 4.23 E.coli >10 h Mamalian 1.1 h FKCRRWQWRMKKLG f(17–42) of lactoferrin 7.9 11.84 3196.9 −0.485 75.31 52.69 Yeast 3 min 2.57 APSITCVRRAFA E.coli 2 min S5 Table S1.