2213 Missouri Ave Carthage, Mo 64836 800-970-4479 A Business of ESM Technologies, LLC stratumnutrition.com Research Summary Research & Patent Portfolio for BLIS Oral-cavity Probiotics: BLIS K12™ & BLIS M18™ Rev. Date: Wednesday, May 13, 2020 Research & Patent Portfolio for BLIS Probiotics: BLIS K12™ & BLIS M18™ B L I S K 12 and B L I S M18 are h i gh ly ad va nce d pro bi o t i cs f o r t h e o ral ca vi t y and upper resp i rat o ry t ract . B L I S K 12 co lo ni ze s i n t h e m o ut h and h elps t o m ai nt ai n ear, no se and t h ro at ( E N T ) i m m une h ealt h , w h i le B L I S M18 su ppo rt s t o o t h and gum h ealt h . B L I S pro bi o t i cs w ere o ri gi nally d i sco ve red as sci ent i st s st ud i ed t h e o ral m i cr o bi o m e o f a ch i ld w i t h ex ce pt i o nal t h ro at h ealt h . B o t h B L I S K 12 and B L I S M18, o r S treptococcus salivarius ( S . salivarius) , w ere i so lat ed f ro m t h e m o ut h s of healthy humans and can help to establish or re-establish a healthy balance of beneficial bacteria in the oral cavity and upper resp i rat o ry t ract . Published Research in Peer-reviewed Journals: ENT Health 1) P as s ari ello C , D i N ard o F, P o li m eni A, D i N ard o D , T es t arelli L . ( 2 02 0) P ro bi o t i c S treptococcus salivarius red uc es s y m p- t o m s o f d ent ure s t o m at i t i s and o ral c o lo ni z at i o n by C andida albicans. Applied S ciences, 10: 3002 . 2 ) B ert uc c i o li A, R o c c h i M, Mo rgant i I , V i c i G , G erv as i M, Am at o ri S and Si s t i D . ( 2 019) S treptococcus salivarius K 12 i n ph a- ry ngo t o ns i lli t i s and ac ut e o t i t i s m ed i a – a m et a- analy s i s . N utrafoods, 2 : 80- 88. 3) Marini G, Sitzia E, Panatta ML and De Vincentiis GC. (2019) Pilot study to explore the prophylactic efficacy of oral probiot- i c S treptococcus salivarius K 12 i n prev ent i ng rec urrent ph ary ngo - t o ns i llar epi s o d es i n ped i at ri c pat i ent s . I nternational J ournal of G eneral M edicine, 12 : 2 13- 2 17 . 4) H u L , Mao Q , Z h o u P , L v X , H ua H and Y an Z . ( 2 019) E f f ec t s o f S treptococcus salivarius K 12 w i t h ny s t at i n o n o ral c and i d i - as i s – R C T . O ral D iseases 2 5( 6) : 157 3- 1580. 5) D i P i erro F, R i s s o P , P o ggi E , T i Mi t i lli A, B o llo li S, B runo M, C anev a E , C am pus R and G i annat t as i o A. ( 2 018) U s e o f S treptococcus salivarius K 12 t o red uc e t h e i nc i d enc e o f ph ary ngo - t o ns i lli t i s and ac ut e o t i t i s m ed i a i n c h i ld ren: a ret ro s pec t i v e analy s i s i n no t - rec urrent ped i at ri c s ubj ec t s . M inerva P ediatrica, 7 0( 3) : 2 40- 2 45. 6) D i P i erro F, C o lo m bo M, G i uli ani MG , D anz a ML , B as i le I , B o llani , T , C o nt i AM, Z anv i t A, and R o t t o li AS. ( 2 016) E f f ec t o f ad m i ni s t rat i o n o f S treptococcus salivarius K 12 o n t h e o c c urrenc e o f s t rept o c o c c al ph ary ngo - t o ns i lli t i s , s c arlet f ev er, and ac ut e o t i t i s m ed i a i n 3 y ears o ld c h i ld ren. European R eview for M edical and P h armacological S ciences, 2 0: 4601- 4606. 7 ) D i P i erro F, C o lo m bo M, Z anv i t A, and R o t t o li AS. ( 2 016) P o s i t i v e c li ni c al o ut c o m es d eri v ed f ro m us i ng S treptococcus sali- varius K 12 t o prev ent s t rept o c o c c al ph ary ngi t i s i n c h i ld ren: a pi lo t i nv es t i gat i o n. D rug H ealth care and P atient S afety , 8: 7 7 - 81. 8) G rego ri G , R i gh i O , R i s s o P , B o i ard i G , D em uru G , Ferz et t i A, G alli A, G h i s o ni M, L enz i ni S, Marengh i C , Mura C , Sac c h et t i R and Suz z ani L . ( 2 016) R ed uc t i o n o f gro up A bet a- h em o ly t i c s t rept o c o c c us ph ary ngo - t o ns i llar i nf ec t i o ns as s o c i at ed w i t h us e o f t h e o ral pro bi o t i c S treptococcus salivarius K 12 : a ret ro s pec t i v e o bs erv at i o nal s t ud y . T h erapeutics and C linical R isk M an- agement, 12 : 87 - 92 . stratumnutrition.com [email protected] (800) 970-4479 * T h es e s t at em ent s h av e no t been ev aluat ed by t h e Fo o d and D rug Ad m i ni s t rat i o n. T h i s pro d uc t i s no t i nt end ed t o d i agno s e, t reat , c ure, o r prev ent any d i s eas e. ENT Health, continued 9) Di Pierro F, Di Pasquale D and Di Cicco M. (2015) Oral use of Streptococcus salivarius K12 in children with secretory otitis media: preliminary results of a pilot, uncontrolled study. International Journal of General Medicine, 8:303-308. 10) Di Pierro F, Colombo M, Zanvit A, Risso P and Rottoli A. (2014) Use of Streptococcus salivarius K12 in the prevention of streptococcal and viral pharyngotonsillitis in children. Drug, Healthcare and Patient Safety, 6:15-20. 11) Di Pierro F, Adami T, Rapacioli G, Giardini N and Streitberger C. (2013) Clinical evaluation of the oral probiotic Strepto- coccus salivarius K12 in the prevention of recurrent pharyngitis and/or tonsillitis caused by Streptococcus pyogenes in adults. Expert Opinion Biological Therapy, 13(3):339-343. 12) Fiedler T, Riani C, Koczan D, Standar K, Kreikemeyer B and Podbielski A. (2013) Protective mechanisms of respiratory tract streptococci against Streptococcus pyogenes biofilm formation and epithelial cell infection. Applied and Environmental Microbiology, 79:1265-1276. 13) Di Pierro F, Donato G, Fomia F, Adami T, Careddu D, Cassandro C and Albera R. (2012) Preliminary pediatric clinical evaluation of the oral probiotic Streptococcus salivarius K12 in preventing recurrent pharyngitis and/or tonsillitis caused by Streptococcus pyogenes and recurrent acute otitis media. International Journal of General Medicine, 5:991-997. 14) Ishijima S, Hayama K, Burton J, Reid G, Okada M, Matsushita Y and Abe S. (2012) Effect of Streptococcus salivarius K12 on the in vitro growth of Candida albicans and its protective effect in an oral candidiasis model. Applied Environmental Microbiology, 78(7):2190-2199. 15) Guglielmetti S, Taverniti V, Minuzzo M, Arioli S, Stuknyte M, Karp M and Mora D. (2010) Oral bacteria as potential probi- otics for the pharyngeal mucosa.