Convergent evolution of the army ant syndrome and congruence in big-data phylogenetics – Supplementary figures and tables Marek L. Borowiec 1 Harpegnathos_saltator_Genome Linepithema_humile_Genome 100 Camponotus_floridanus_Genome 100 Pogonomyrmex_barbatus_Genome Vollenhovia_emeryi_Genome 100 100 Cardiocondyla_obscurior_Genome 100 Solenopsis_invicta_Genome 100 Atta_cephalotes_Genome 100 Acromyrmex_echinatior_Genome Tanipone_scelesta_M159 100 Tanipone_zona_M182 100 Tanipone_hirsuta_M279 100 Tanipone_aglandula_M280 Lioponera_longitarsus_M193 Lioponera_vespula_M210 100100 Lioponera_nr_kraepelinii_M131 100 Lioponera_nr_mayri_M215 100 Lioponera_MY_M268 100 Lioponera_cf_suscitata_M267 100 Lioponera_PG_M250 100 Lioponera_marginata_M249 87 Lioponera_princeps_M137 100 100 Lioponera_ruficornis_M165 Lividopone_MG_M132 100 100 Lividopone_MG_M194 100Lividopone_MG_M216 66 Lividopone_MG_M294 Zasphinctus_MZ_M229 100 Zasphinctus_KE_M227 100 100 Zasphinctus_TH_M192 100 Zasphinctus_trux_M136 100 Zasphinctus_PG_M285 100 100 Zasphinctus_imbecilis_M170 Parasyscia_UG_M281 100 Parasyscia_MG_M212 Parasyscia_UG_M219 100 100 Parasyscia_UG_M218 100 100 Parasyscia_wittmeri_M282 83 Parasyscia_VN_M207 100 Parasyscia_PG_M204 100 Parasyscia_dohertyi_M142 Vicinopone_conciliatrix_M128 Simopone_grandidieri_M186 83 100 Simopone_cf_oculata_D0792 100 Simopone_dryas_M224 100 Simopone_marleyi_M171 100 Simopone_conradti_M144 93 Simopone_rex_M133 100 Simopone_trita_M185 Syscia_augustae_M196 100 Syscia_GT_M127 100 Syscia_typhla_D0841 100 Syscia_MY_M176 100 100 Syscia_MY_M147 Eusphinctus_TH_M158 83 93 Ooceraea_fragosa_D0842 Ooceraea_biroi_Genome 100 100 Ooceraea_australis_M138 100 Ooceraea_MY_M270 100 Ooceraea_PG_M248 100 Ooceraea_FJ06_M168 Eburopone_CM02_D0788 100 Eburopone_UG_M259 Eburopone_MG_M195 100 100 Eburopone_MG_M130 100 Eburopone_MG_M213 100 Eburopone_MG_M214 Yunodorylus_TH_M160 100 98 Yunodorylus_paradoxus_M190 100 Yunodorylus_TH_M191 100 Yunodorylus_eguchii_M247 100 Cerapachys_sulcinodis_M243 100 Cerapachys_MY_M264 100 Cerapachys_antennatus_M263 100 84 Cerapachys_TH_M203 Chrysapace_TH_M156 100 Chrysapace_cf_crawleyi_M262 100 Chrysapace_MG_M155 100 Chrysapace_cf_sauteri_M261 Aenictogiton_ZM02_M181 100 Aenictogiton_UG_M189 97 100 Dorylus_laevigatus_M157 Dorylus_orientalis_M244 99 100100 Dorylus_conradti_M178 100 Dorylus_fimbriatus_M288 Dorylus_cf_fulvus_M149 100 100 Dorylus_fulvus_M179 100 Dorylus_spininodis_M292 Dorylus_braunsi_M289 100100 Dorylus_affinis_M177 97 100Dorylus_kohli_M180 100Dorylus_mayri_M291 100Dorylus_molestus_M290 100Dorylus_rubellus_M287 93 Dorylus_UG_M153 Aenictus_silvestrii_M221 100 Aenictus_yamanei_M265 99 Aenictus_latifemoratus_M266 49 Aenictus_bobaiensis_M197 100 100 Aenictus_levior_M231 Aenictus_camposi_M222 Aenictus_UG_M143 100 100 Aenictus_ZA_M228 99 Aenictus_fergusoni_M223 100 100 Aenictus_hodgsoni_M198 100Aenictus_laeviceps_M269 100 Aenictus_rotundicollis_M232 100 Aenictus_turneri_M135 100Aenictus_fuchuanensis_M201 100 100 Aenictus_hoelldobleri_M200 Aenictus_currax_M245 100Aenictus_cornutus_M230 100 Aenictus_gracilis_M199 Neocerapachys_CR_M209 100 Neocerapachys_neotropicus_M134 100Neocerapachys_cf_splendens_M251 100 Neocerapachys_BR_M295 97 Cylindromyrmex_meinerti_D778 100 Cylindromyrmex_brasiliensis_M140 100 Cylindromyrmex_whymperi_M271 100 100 Cylindromyrmex_darlingtoni_M211 Acanthostichus_AZ_M277 100 Acanthostichus_AZ_M278 100 Acanthostichus_BR_M252 100Acanthostichus_GF_M208 100 98 Acanthostichus_serratulus_M166 Leptanilloides_nubecula_M167 100 Leptanilloides_mckennae_D0228 100 Leptanilloides_femoralis_M188 100 Leptanilloides_gracilis_M187 100 Leptanilloides_erinys_M246 Sphinctomyrmex_stali_M253 100 Sphinctomyrmex_marcoyi_M202 98 Cheliomyrmex_PE_M146 100Cheliomyrmex_cf_andicola_M174 96 Cheliomyrmex_cf_morosus_M217 100 Labidus_coecus_M173 100 Labidus_spininodis_M172 100 97 Labidus_praedator_M152 Nomamyrmex_hartigii_M220 100 100 Nomamyrmex_esenbecki_M129 100 Eciton_mexicanum_M238 61Eciton_vagans_M184 100 100Eciton_quadriglume_M254 Eciton_lucanoides_M239 100 100Eciton_burchellii_M273 100Eciton_hamatum_M293 100 Eciton_rapax_M145 Neivamyrmex_distans_M242 100 Neivamyrmex_alfaroi_M240 100 Neivamyrmex_swainsonii_M169 100 Neivamyrmex_adnepos_M236 100 Neivamyrmex_EC_M175 100 Neivamyrmex_gibbatus_M139 100 Neivamyrmex_impudens_M237 100 0.04 100 Neivamyrmex_melanocephalus_M154 Neivamyrmex_cf_nyensis_M296 combined data matrix 100 Neivamyrmex_compressinodis_M235 Neivamyrmex_sumichrasti_M151 100100 maximum likelihood tree Neivamyrmex_asper_M233 100Neivamyrmex_kiowapache_M275 RAxML 100Neivamyrmex_opacithorax_M276 96Neivamyrmex_texanus_M274 partitioned by locus GTR+4Gamma model 100 Neivamyrmex_californicus_M272 500 bootstrap replicates Supplementary Figure S1: Maximum likelihood tree obtained from the combined data matrix par- titioned by locus. Scale is in number of substitutions per site. Nodal support in percent bootstrap. 2 Harpegnathos_saltator_Genome Linepithema_humile_Genome 100 Camponotus_floridanus_Genome 100 Pogonomyrmex_barbatus_Genome Vollenhovia_emeryi_Genome 100 100 Cardiocondyla_obscurior_Genome 100 Solenopsis_invicta_Genome 100 Atta_cephalotes_Genome 100 Acromyrmex_echinatior_Genome Aenictogiton_ZM02_M181 100 Aenictogiton_UG_M189 100 Dorylus_laevigatus_M157 Dorylus_orientalis_M244 100100 Dorylus_conradti_M178 100 Dorylus_fimbriatus_M288 Dorylus_fulvus_M179 100 100 Dorylus_cf_fulvus_M149 100 Dorylus_spininodis_M292 Dorylus_affinis_M177 100100 Dorylus_braunsi_M289 100Dorylus_kohli_M180 100Dorylus_mayri_M291 100Dorylus_molestus_M290 100Dorylus_rubellus_M287 100 Dorylus_UG_M153 Aenictus_silvestrii_M221 Aenictus_latifemoratus_M266 10090 Aenictus_yamanei_M265 96 Aenictus_bobaiensis_M197 100 100 Aenictus_levior_M231 Aenictus_camposi_M222 Aenictus_ZA_M228 100 100 Aenictus_UG_M143 Aenictus_fergusoni_M223 100 100 Aenictus_hodgsoni_M198 100Aenictus_laeviceps_M269 100 Aenictus_rotundicollis_M232 100 Aenictus_turneri_M135 100Aenictus_fuchuanensis_M201 100 100 100 Aenictus_hoelldobleri_M200 Aenictus_currax_M245 100Aenictus_cornutus_M230 100 100 Aenictus_gracilis_M199 Cheliomyrmex_PE_M146 100Cheliomyrmex_cf_andicola_M174 100 Cheliomyrmex_cf_morosus_M217 100 Labidus_coecus_M173 100 Labidus_spininodis_M172 100 Labidus_praedator_M152 100 Nomamyrmex_hartigii_M220 100 Nomamyrmex_esenbecki_M129 100 Eciton_mexicanum_M238 Eciton_vagans_M184 100100 Eciton_quadriglume_M254 100 100 Eciton_lucanoides_M239 100Eciton_burchellii_M273 100Eciton_hamatum_M293 100 Eciton_rapax_M145 Neivamyrmex_distans_M242 100 Neivamyrmex_alfaroi_M240 99 Neivamyrmex_swainsonii_M169 100 Neivamyrmex_adnepos_M236 100 Neivamyrmex_EC_M175 100 Neivamyrmex_gibbatus_M139 100 Neivamyrmex_impudens_M237 100 99 Neivamyrmex_melanocephalus_M154 Neivamyrmex_cf_nyensis_M296 82 100 Neivamyrmex_compressinodis_M235 Neivamyrmex_sumichrasti_M151 100100 Neivamyrmex_asper_M233 100Neivamyrmex_kiowapache_M275 100Neivamyrmex_opacithorax_M276 100Neivamyrmex_texanus_M274 100 Neivamyrmex_californicus_M272 Yunodorylus_TH_M160 100 Yunodorylus_paradoxus_M190 100 Yunodorylus_TH_M191 100 Yunodorylus_eguchii_M247 Chrysapace_TH_M156 100 100 Chrysapace_cf_crawleyi_M262 100 Chrysapace_MG_M155 100 89 Chrysapace_cf_sauteri_M261 Cerapachys_sulcinodis_M243 100 Cerapachys_MY_M264 100 Cerapachys_antennatus_M263 100 Cerapachys_TH_M203 Sphinctomyrmex_stali_M253 100 Sphinctomyrmex_marcoyi_M202 Leptanilloides_nubecula_M167 96 100 Leptanilloides_mckennae_D0228 100 Leptanilloides_femoralis_M188 100 Leptanilloides_gracilis_M187 100 100 Leptanilloides_erinys_M246 100 Neocerapachys_CR_M209 100 Neocerapachys_neotropicus_M134 100Neocerapachys_cf_splendens_M251 100 Neocerapachys_BR_M295 96 Cylindromyrmex_meinerti_D778 100 Cylindromyrmex_brasiliensis_M140 100 Cylindromyrmex_whymperi_M271 100 100 Cylindromyrmex_darlingtoni_M211 Acanthostichus_AZ_M277 100 Acanthostichus_AZ_M278 100 Acanthostichus_BR_M252 100Acanthostichus_GF_M208 100 100 Acanthostichus_serratulus_M166 Eburopone_CM02_D0788 100 Eburopone_UG_M259 Eburopone_MG_M195 100 100 Eburopone_MG_M130 100 Eburopone_MG_M213 100 Eburopone_MG_M214 Syscia_GT_M127 100 Syscia_augustae_M196 100 Syscia_typhla_D0841 100 Syscia_MY_M176 100 83 100 Syscia_MY_M147 Eusphinctus_TH_M158 96 Ooceraea_fragosa_D0842 Ooceraea_biroi_Genome 100 100 Ooceraea_australis_M138 100 Ooceraea_MY_M270 100 Ooceraea_FJ06_M168 100 Ooceraea_PG_M248 100 Vicinopone_conciliatrix_M128 Simopone_grandidieri_M186 64 100 Simopone_cf_oculata_D0792 100 Simopone_dryas_M224 Simopone_rex_M133 100 100 Simopone_trita_M185 100 Simopone_conradti_M144 100 Simopone_marleyi_M171 88 Tanipone_scelesta_M159 100 Tanipone_zona_M182 100 Tanipone_hirsuta_M279 100 Tanipone_aglandula_M280 Lioponera_longitarsus_M193 Lioponera_vespula_M210 100100 Lioponera_nr_kraepelinii_M131 100 64 Lioponera_nr_mayri_M215 100 Lioponera_MY_M268 100 Lioponera_cf_suscitata_M267 100 Lioponera_PG_M250 100 Lioponera_marginata_M249 41 Lioponera_princeps_M137 100 100 Lioponera_ruficornis_M165 Lividopone_MG_M132 100 Lividopone_MG_M194 100Lividopone_MG_M216 100 Lividopone_MG_M294 Zasphinctus_MZ_M229 100 Zasphinctus_KE_M227 100 100 Zasphinctus_TH_M192 100 Zasphinctus_trux_M136 100 Zasphinctus_PG_M285 100 100 Zasphinctus_imbecilis_M170 0.07 Parasyscia_UG_M281 100 Parasyscia_MG_M212 combined data matrix Parasyscia_UG_M219 100 100 Parasyscia_UG_M218 100 maximum likelihood tree 100 Parasyscia_wittmeri_M282 Parasyscia_VN_M207 RAxML 100 Parasyscia_PG_M204 100 k-means partitions GTR+4Gamma model Parasyscia_dohertyi_M142 500 bootstrap replicates Supplementary Figure S2: Maximum likelihood tree obtained from the combined data matrix under k-means partitions. Scale is in number of