PRAJNA - Journal of Pure and Applied Sciences ISSN 0975 2595 Volume 19 December 2011 CONTENTS BIOSCIENCES Altered energy transfer in Phycobilisomes of the Cyanobacterium, Spirulina Platensis under 1 - 3 the influence of Chromium (III) Ayya Raju, M. and Murthy, S. D. S. PRAJNA Volume 19, 2011 Biotransformation of 11β , 17 α -dihydroxy-4-pregnene-3, 20-dione-21-o-succinate to a 4 - 7 17-ketosteroid by Pseudomonas Putida MTCC 1259 in absence of 9α -hydroxylase inhibitors Rahul Patel and Kirti Pawar Influence of nicking in combination with various plant growth substances on seed 8 - 10 germination and seedling growth of Noni (Morinda Citrifolia L.) Karnam Jaya Chandra and Dasari Daniel Gnana Sagar Quantitative analysis of aquatic Macrophytes in certain wetlands of Kachchh District, 11 - 13 Journal of Pure and Applied Sciences Gujarat J.P. Shah, Y.B. Dabgar and B.K. Jain Screening of crude root extracts of some Indian plants for their antibacterial activity 14 - 18 Purvesh B. Bharvad, Ashish R. Nayak, Naynika K. Patel and J. S. S. Mohan ________ Short Communication Heterosis for biometric characters and seed yield in parents and hybrids of rice 19 - 20 (Oryza Sativa L.) M. Prakash and B. Sunil Kumar CHEMISTRY Adsorption behavior and thermodynamics investigation of Aniline-n- 21 - 24 (p-Methoxybenzylidene) as corrosion inhibitor for Al-Mg alloy in hydrochloric acid V.A. Panchal, A.S. Patel and N.K. Shah Grafting of Butyl Acrylate onto Sodium Salt of partially Carboxymethylated Guar Gum 25 - 31 using Ceric Ions J.H. Trivedi, T.A. Bhatt and H.C. Trivedi Simultaneous equation and absorbance ratio methods for estimation of Fluoxetine 32 - 36 Hydrochloride and Olanzapine in tablet dosage form Vijaykumar K. Parmar, Uday H. Thorat, Girish K. Jani and Laxman M. Prajapati COMPUTER SCIENCE Bootstrapping of phone models for a large vocabulary continuous speech recognition for 37 - 40 Gujarati Himanshu N. Patel and Paresh V. Virparia SARDAR PATEL UNIVERSITY VALLABH VIDYANAGAR Natural Language Interface for Student Information System (NLSIS) 41 - 44 Gujarat - 388 120, INDIA Amisha Shingala, Rinku Chavda and Paresh Virparia www.spuvvn.edu Sardar Patel University Press, Vallabh Vidyanagar, Gujarat - 388 120, INDIA PRAJNA - Journal of Pure and Applied Sciences MATHEMATICS A Mathematical model for a growth curve of rice crop 45 - 46 (Abbreviation: PRAJNA - J. Pure & Appl. Sci.) R. Lakshmi and V. Ravichandran On a new sequence of functions defined by a generalized Rodrigues formula 47 - 49 J.C. Prajapati, N.K. Ajudia and A.K. Shukla __________ Patron Corrected Version of Vol. 18: 115-116 (2010) Mean labeling for some new families of graphs 50 - 51 Prof. Harish Padh S.K. Vaidya and Lekha Bijukumar Vice-Chancellor PHYSICS Correlations among atomic properties and estimates on exotic atoms 52 - 55 Pooja Bhowmik, Ramji H. Patel and K.N. Joshipura Managing Editor Crystal quartz windows for far-infrared interferometer of SST-1 56 - 59 Asha Adhiya and Rajwinder Kaur Prof. T. V. Ramana Rao B. R. D. School of Biosciences Decay widths of B→ J/Ψ p+ in CPP ν model 60 - 62 Sardar Patel University c Arpit Parmar, Kaushal Thakkar, Bhavin Patel and P.C. Vinodkumar Vallabh Vidyanagar, Gujarat - 388 120 [email protected] DFT studies on electronic structure and elastic properties of CoN: Zincblende and Rocksalt Structures 63 - 67 Jameson Maibam, B. Indrajit Sharma, Ramendu Bhattacharjee, R.K. Thapa and R.K. Brojen Singh Editorial Board Electrical transport properties of Tungsten Ditelluride (WTe2 ) Crystals 68 - 70 Prof. S. J. Bhatt Prof. Rema Subhash Prof. D. K. Raval Priyanka Desai, D.D. Patel, D.N. Bhavsar and A.R. Jani Dept. of Mathematics Dept. of Home Science Department of Chemistry [email protected] [email protected] [email protected] Electron impact total cross sections for Ethylene Oxide 71 - 74 Avani Y. Barot, Minaxi Vinodkumar and Bobby Antony Prof. K. N. Joshipura Prof. Satish Manocha Prof. D. Lakshminarayana 75 - 78 Dept. of Physics Dept. of Materials Science Department of Electronics Infrared spectra of charge transfer complexes of Proteoglycan [email protected] [email protected] [email protected] Pravinsinh I. Rathod, Vishal Patel and A.T. Oza Optical Properties of Tin Monosulphide And Tin Monoselenide Single Crystals 79 - 81 Prof. K. S. Rao Prof. Darshan Choksi Prof. Ashok Shanubhogue B.B. Nariya, A.K. Dasadia and A.R. Jani Dept. of Biosciences Dept. of Computer Science Department of Statistics [email protected] [email protected] [email protected] Structural Characterization and Transport Properties of CVT Grown ZrSe3 and ZrS3 Crystals 82 - 84 A.K. Dasadia, B.B. Nariya and A.R. Jani Theoretical calculation of electron impact total cross sections for HCl molecule over a Since 1991 Sardar Patel University has been regularly publishing the PRAJÑA - Journal of Pure wide range of impact energies (0.1 ev – 2000 ev) 85 - 89 and Applied Sciences with an aim of providing a platform to Academicians, Researchers and Mayuri Y. Barot, Harshad Bhutadia, Minaxi Vinodkumar Scientists for the rapid dissemination of original scientific work carried out in the University Departments, Affiliated Colleges and Research Laboratories located in Gujarat. All articles STATISTICS published in PRAJNA are deemed to reflect the individual views of the authors and not the official points of view of the Sardar Patel University. A case study of hybrid microcircuit assembly process using Taguchi design method and response surface method 90 - 92 D. P. Raykundaliya and A. Shanubhogue Acceptance decision rule for Type-I generalized half logistic distribution 93 - 96 A Peer Reviewed Journal K. Rosaiah, R.R.L. Kantam and V. Rama Krishna Indexed in Indian Science Abstracts On variance estimation for the Greg estimator 97 - 99 Published by the Registrar, Sardar Patel University, Vallabh Vidyanagar, Gujarat - 388 120 P.A. Patel and R.D. Chaudhari Printed at Sardar Patel University Press, Vallabh Vidyanagar, Gujarat-388 120 Guidelines for Contributors 100 - 101 PRAJNA - Journal of Pure and Applied Sciences Volume 19, December 2011 Sardar Patel University is grateful to Prof. Vithalbhai A. Patel Humboldt State University, Arcata, Califonia, USA, whose generous donation to organize academic activities in the name of Shri Ishwarbhai Ambalal Patel (Shertha) helped to meet a part of tghe publication cost of PRAJNA Journal. ***** The Editorial Board of PRAJNA is grateful to all those experts who rendered their valuable services as referees for reviewing the papers received for this volume of the Journal. ***** An Official Publication of Sardar Patel University The computational assistance received from Miss. Jigisha Vaghela and Mr. Sahaj Gandhi, Vallabh Vidyanagar, Gujarat - 388 120, India Doctoral Research Students of of Biosciences and Physics Departments respectively for making of this volume of www.spuvvn.edu E-mail: [email protected] PRAJNA Journal is also gratefully acknowledged. PRAJÑĀ-Journal of Pure and Applied Sciences, Vol. 19: 1 - 3 (2011) ISSN 0975 - 2595 ALTERED ENERGY TRANSFER IN PHYCOBILISOMES OF THE CYANOBACTERIUM, SPIRULINA PLATENSIS UNDER THE INFLUENCE OF CHROMIUM (III) Ayya Raju, M. and Murthy, S.D.S.* Department of Biochemistry, S.V. University, Tirupati-517 502, A.P., India ABSTRACT Phycobilisomes act as major light harvesting complex in photosystem II of cyanobacteria. In this investigation an attempt has been made to study the effect of chromium (Cr) in the energy transfer of phycobilisomes. Our results indicate that chromium (III) (50 and 100 µM) is able to cause alterations in absorption and energy transfer with intact cells at 100 µM concentration during short term incubation. The phycobilisomes isolated from chromium (50uM) treated cells also exhibited changes in absorption as well as fluoroscence emission properties in the red shift in the peak position. Thus chromium acts as an energy transfer inhibitor both under in vitro and in vivo conditions. Key worlds : Absorption, Allophycocyanin, Cyanobacteria, Fluorescence, Phycocyanin. Fig. 1 Effect of 'Cr' ions on the absorption spectra of intact cells of Spirulina platensis. Fig. 2 Effect of ' Cr' on phycocyanin fluorescence emission spectra of the intact cells. RESULTS AND DISCUSSION: Initially after giving the Cr ions (100 µM) treatment for 10 min, the absorption characteristics of different pigment proteins present in the control cells of Spirulina platensis was measured. The peak at 433 nm is due to the soret band of Chl a; at 489nm the peak is due to carotenoids, at 621 nm is due to the absorption of PC and peak at 679 nm is due to the absorption of Chl a [15]. The *Corresponding author: [email protected] 2 PRAJÑ Ā- Journal of Pure and Applied Sciences, Vol. 19: 1 - 3 (2011) December (50 µM) Spirulina cells were used for the measurement of phycocyanin fluorescence emission. Chromium is able to cause 42% decrease in the fluorescence intensity and blue shift in the emission peak from 670 nm to 666 nm. The decrease in the fluorescence intensity indicates the change in the energy transfer and blue shift gives information about structural changes in the PBSs (Fig 4). Similar reports were made by Murthy et al [14] during the toxic effect of mercuric chloride (HgCl2 ) on the spectral properties of phycobiliproteins in the same organism. Thus chromium is able to cause alterations in the energy transfer from PC to Chl a in the Spirulina both under in vivo as well as in vitro conditions by inducing changes in PBSs. Fig. 3 Absorption spectra of isolated PBSs from control and 'Cr' ACKNOWLEDGEMENT treated cells. Prof. S. D. S. Murthy is thankful to University Grants Commission for providing Major Research Project (2011-2014). REFERENCES [1] Gantt, E. (1981) Phycobi1isomes. Ann. Rev. Plant Physiol. 32: 327-347. [2] Glazer, A. (1984) Phycobilisome: A macromolecular complex specialized for light energy transfer. Biochim. Biophys. Acta 768, 29. [3] Grossman, A .R., Schaefer, M.R., Chiang, G. G. and Collier, J.