Authors: Pallavi Subhraveti Ron Caspi Peter Midford Peter D Karp An online version of this diagram is available at BioCyc.org. Biosynthetic pathways are positioned in the left of the cytoplasm, degradative pathways on the right, and reactions not assigned to any pathway are in the far right of the cytoplasm. Transporters and membrane proteins are shown on the membrane. Ingrid Keseler Periplasmic (where appropriate) and extracellular reactions and proteins may also be shown. Pathways are colored according to their cellular function. Gcf_004006635Cyc: Fusobacterium necrophorum necrophorum ATCC 25286 Cellular Overview Connections between pathways are omitted for legibility. Anamika Kothari Macromolecule Modification Cofactor, Prosthetic Group, Electron Carrier, and Vitamin Biosynthesis Amino Acid Degradation Aldehyde Carbohydrate Biosynthesis Degradation core a peptidoglycan 5'-chloro-5'- UDP-N-acetyl- tRNA processing L-histidine degradation III cob(II)alamin- M-antigen a nucleoside a ribonucleoside L,L- cob(II)yrinate a,c- L-threonine D-serine methylmalonyl- a purine oligosaccharide- glycerophosphoserine a ribonucleic acid a purine α-D-glucosamine oxaloacetate a malonyl-[acp] cob(II)yrinate a,c-diamide flavin biosynthesis I (bacteria and plants) adenosylcobinamide-GDP adenosylcobalamin tetrahydrofolate biosynthesis 10 glutathione- methylglyoxal ATP [CblC-CblD ATP cob(II)inamide dimer with triphosphate deoxyadenosine diphosphate diamide biosynthesis I adenosylcobinamide- N -formyl-tetrahydrofolate biosynthesis di-trans,poly-cis O-antigen building blocks biosynthesis (E. coli) degradation I degradation CoA mutase ribonucleoside adenosylcobinamide (variant) monomer glycerophosphodiester ribonucleoside purine-nucleoside UDP-N- diaminopimelate oxaloacetate biosynthesis II (late salvage from cobinamide I salvage from cobalamin glutaredoxin ADP-L-glycero-β- UDP-α-D-galactose degradation I complex] lipid A (E. coli pentapeptide DNA-directed ribonucleotide- diaminopimelate beta- (early cobalt insertion) GDP biosynthesis from (7,8-dihydropterin- -undecaprenyl a polycistronic cob(I)yrinic acid cob(I)yrinic acid a,c-diamide 5'-triphosphate bifunctional undecaprenyl phosphodiesterase: purine-nucleoside phosphorylase: acetylglucosamine decarboxylase: cobalt incorporation) α α biosynthesis his K-12 core type) stems (meso- RNA polymerase diphosphate epimerase: ketoacyl- cobyrinate a,c-diamide redox reactions phosphate biosynthesis UDP- -D- -D- D-glucopyranose D-manno-heptose tRNA precursor adenosyltransferase: diphosphate O-antigen phosphorylase: 2-epimerase (non- uroporphyrinogen-III GTP cob(II)inamide cob(I)alamin 6-yl)methyl 4-aminobenzoate a tetrahydrofolate thr D-serine methylglyoxal a,c-diamide adenosylcobinamide (no GlcNAc) diaminopimelateN-acetylmuramoyl- EO219_RS06925 deoD reductase: EO219_RS11900 EO219_RS06990 uroporphyrinogen-III cob(II)yrinate (2E,6E)-farnesyl galactose glucopyranose 6-phosphate biosynthesis subunit alpha: deoD [acyl- uroporphyrinogen-III cob(I)yrinic acid diphosphate glutathione IPP glucose-6- UDP-α-D-glucose histidine ammonia- lactoylglutathione adenosyltransferase: EO219_RS00500 kinase/ ligase domain- containing)L-alanine amidase: glycerophosphodiester purine-nucleoside EO219_RS00225 hydrolyzing): oxaloacetate uroporphyrinogen-III a,c-diamide diphosphate 1-phosphate D-sedoheptulose threonine D-serine L-methionine EO219_RS01280 diaminopimelate carrier- C-methyltransferase: cob(I)yrinic acid a,c-diamide 7,8- glucose-1-phosphate phosphate UDP-glucose lyase: hutH lyase: EO219_RS07480 cob(I)yrinic acid a,c-diamide adenosylcobinamide- containing protein: EO219_RS01050 phosphodiesterase: purine-nucleoside phosphorylase: EO219_RS00230 EO219_RS04535 decarboxylase C-methyltransferase: bifunctional UDP-Galf 7-phosphateD-sedoheptulose ammonia-lyase: ammonia- degradation II DNA- a 2'- epimerase: protein] cobA a,c-diamide adenosyltransferase: dihydropteroate 5-methyltetrahydrofolate- di-trans,octa-cis thymidylyltransferase: isomerase: 4-epimerase EO219_RS08920 cob(I)yrinic acid adenosyltransferase: phosphate EO219_RS04680 N-acetylmuramoyl- EO219_RS00215 phosphorylase: deoD UDP-N- subunit alpha: cobA 3,4-dihydroxy- cob(II)alamin histidine ammonia- (2Z)-2-aminobut-EO219_RS11680 lyase: (R)-S- directed RNA deoxyribonucleoside EO219_RS01660 synthase adenosyltransferase: -homocysteine a reduced -undecaprenyl rfbA 7-phosphate GalE: galE a tRNA precursor a tRNA precursor EO219_RS07480 guanylyltransferase: a peptidoglycan deoD 5-chloro-5- precorrin-1 precorrin-1 2-butanone- EO219_RS07480 bifunctional dTDP-α-D-glucose EO219_RS01795 lyase: hutH dsdA a,c-diamide O-antigen L-alanine amidase: sn-glycerol α-D-ribose- 5'-diphosphate acetylglucosamine meso- EO219_RS06985 II: fabF EO219_RS07480 methyltransferase: glutaredoxin isomerase: gmhA with a 5' with a 5' 2-enoate 2-aminoprop- met lactoylglutathione EO219_RS04435 L-alanyl-γ-D- dimer with ser polymerase: adenine a 3- cob(I)yrinic acid cob(I)yrinic acid folylpolyglutamate diphosphate F6P D-glycero-D- [+ 2 isozymes] adenosyltransferase: triphosphate adenosylcobinamide a purine adenosyl- ligase domain- EO219_RS02760 3-phosphate a purine base deoxyribose 2-epimerase (non- diaminopimelate pyruvate uroporphyrinogen-III 4-phosphate EO219_RS10045 dTDP-glucose α extension and extension and a 2-enoate methionine glutamyl-meso-2,6- pentapeptide rpoB 1-phosphate oxodocosanoyl- uroporphyrinogen-III cob(I)yrinic acid a,c-diamide a,c-diamide synthase/ glutaredoxin: undecaprenyl- manno-heptose UDP- -D-galactose urocanate a [cobalamin EO219_RS00500 adenosylcobalamin- ribonucleoside cobinamide containing protein: 1-phosphate hydrolyzing): C-methyltransferase: synthase/GTP 4,6-dehydratase: glutamine-- a long 3' trailer short 3' extension gamma- a [cyanocobalamin di-trans,octa-cis diaminopimeloyl- stem (meso- rpoC rpoZ [acp] C-methyltransferase: a,c-diamide adenosyltransferase: adenosyltransferase: dihydrofolate dihydrofolate EO219_RS08930 diphosphate 7-phosphate trafficking triphosphate [methylmalonyl- 5'-diphosphate phosphate EO219_RS10720 EO219_RS04775 cobA cyclohydrolase II: EO219_RS04585 fructose-6- D-glycero- ribonuclease urocanate hydratase: lyase: megL reductase CblC] -undecaprenyl E.