The Scientific World Journal Plant Biotechnology Guest Editors: Khalid Mahmood Khawar, Selma Onarici, Cigdem Alev Ozel, Muhammad Aasim, Allah Bakhsh, and Abdul Qayyum Rao Plant Biotechnology The Scientific World Journal Plant Biotechnology Guest Editors: Khalid Mahmood Khawar, Selma Onarici, Cigdem Alev Ozel, Muhammad Aasim, Allah Bakhsh, and Abdul Qayyum Rao Copyright © 2013 Hindawi Publishing Corporation. All rights reserved. This is a special issue published in “The Scientific World Journal.”All articles are open access articles distributed under the Creative Com- mons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Contents Plant Biotechnology, Khalid Mahmood Khawar, Selma Onarici, Cigdem Alev Ozel, Muhammad Aasim, Allah Bakhsh, and Abdul Qayyum Rao Volume 2013, Article ID 736731, 2 pages AComparisonofIceColdWaterPretreatmentand-Bromonaphthalene Cytogenetic Method for Identification of Papaver Species, Amir Rezaei Osalou, Sheida Daneshvar Rouyandezagh, Behrouz Alizadeh, Celal Er, and Cafer Sirri Sevimay Volume 2013, Article ID 608650, 6 pages Induction and Analysis of the Alkaloid Mitragynine Content of a Mitragyna speciosa Suspension Culture System upon Elicitation and Precursor Feeding, Nor Nahazima Mohamad Zuldin, Ikram Md. Said, Normah Mohd Noor, Zamri Zainal, Chew Jin Kiat, and Ismanizan Ismail Volume 2013, Article ID 209434, 11 pages Annotation of Differentially Expressed Genes in the Somatic Embryogenesis of Musa and Their Location in the Banana Genome, Josefina Ines Maldonado-Borges, Jose´ Roberto Ku-Cauich, and Rosa Maria Escobedo-GraciaMedrano Volume 2013, Article ID 535737, 7 pages Genetic Diversity Analysis of Sugarcane Parents in Chinese Breeding Programmes Using gSSR Markers, Qian You, Liping Xu, Yifeng Zheng, and Youxiong Que Volume 2013, Article ID 613062, 11 pages Genetic Transformation in Citrus, Dicle Donmez, Ozhan Simsek, Tolga Izgu, Yildiz Aka Kacar, and Yesim Yalcin Mendi Volume 2013, Article ID 491207, 8 pages Micropropagation of Origanum acutidens (HAND.-MAZZ.) IETSWAART Using Stem Node Explants, Mehmet Ugur Yildirim Volume 2013, Article ID 276464, 3 pages The Influence of 1-Triacontanol on the Growth, Flowering, and Quality of Potted Bougainvillea Plants (Bougainvillea glabra var. “Elizabeth Angus”) under Natural Conditions, Mohammad Moneruzzaman Khandaker, Golam Faruq, M. Motior Rahman, M. Sofian-Azirun, and Amru Nasrulhaq Boyce Volume 2013, Article ID 308651, 12 pages Use of Tissue Culture Techniques for Producing Virus-Free Plant in Garlic and Their Identification through Real-Time PCR, Hatıra Tas¸kın, Gokhan¨ Baktemur, Mehmet Kurul, and Saadet Buy¨ ukalaca¨ Volume 2013, Article ID 781282, 5 pages Adventitious Shoot Regeneration from Leaf Explant of Dwarf Hygro (Hygrophila polysperma (Roxb.) T. Anderson),MehmetKaratas¸, Muhammad Aasim, Ays¸egul¨ C¸ ınar, and Muhammet Dogan Volume 2013, Article ID 680425, 7 pages Protein Profiling and Histone Deacetylation Activities in Somaclonal Variants of Oil Palm (Elaeis guineensis Jacq.), Jamilah Syafawati Yaacob, Hwei-San Loh, and Rosna Mat Taha Volume 2013, Article ID 613635, 8 pages Effects of IAA, IBA, NAA, and GA3 on Rooting and Morphological Features of Melissa officinalis L. Stem Cuttings,HakanSevikandKerimGuney Volume 2013, Article ID 909507, 5 pages High Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. through Solid and Liquid Cultures, Jingli Yang, Songquan Wu, and Chenghao Li Volume 2013, Article ID 718754, 6 pages Comparison of Different Methods for Separation of Haploid Embryo Induced through Irradiated Pollen and Their Economic Analysis in Melon (Cucumis melo var. inodorus),Gokhan¨ Baktemur, Hatıra Tas¸kın, and Saadet Buy¨ ukalaca¨ Volume 2013, Article ID 529502, 7 pages Comparative Studies on Cellular Behaviour of Carnation (Dianthus caryophyllus Linn. cv. Grenadin) Grown In Vivo and In Vitro for Early Detection of Somaclonal Variation, Jamilah Syafawati Yaacob, Rosna Mat Taha, and Arash Khorasani Esmaeili Volume 2013, Article ID 686752, 8 pages Hindawi Publishing Corporation The Scientific World Journal Volume 2013, Article ID 736731, 2 pages http://dx.doi.org/10.1155/2013/736731 Editorial Plant Biotechnology Khalid Mahmood Khawar,1 Selma Onarici,2 Cigdem Alev Ozel,3 Muhammad Aasim,4 Allah Bakhsh,5 and Abdul Qayyum Rao6 1 DepartmentofFieldCrops,AnkaraUniversity,06110Ankara,Turkey 2 The Scientific and Technical Council of Turkey Genetic Engineering and Biotechnology Institute, Gebze, 41470 Kocaeli, Turkey 3 Department of Biology Education, Gazi University, 06500 Ankara, Turkey 4 Department of Biology, Karamanoglu Mehmetbey University, 70200 Karaman, Turkey 5 Department of Agricultural Sciences, Section Plant Molecular Virology, University of Bologna, 40127 Bologna, Italy 6 Centre of Excellence in Molecular Biology (CEMB), University of the Punjab, 87 West Canal Bank Road, Thokar Niaz Baig, Lahore 53700, Pakistan Correspondence should be addressed to Khalid Mahmood Khawar; [email protected] Received 25 August 2013; Accepted 25 August 2013 Copyright © 2013 Khalid Mahmood Khawar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. It is a privilege, pleasure, and honour to present this special One paper presents cytogenetic study of Papaver species issue to the international scientific community. The editorial that introduced a new concept by developing ice cold water boardisconfidentthatitisatrulygoodpublication;asthe instead of bromonaphthalene for chromosome studies. papers published in this issue cover a broad spectrum of The papers on plant cell and tissue culture include a topics on plant biotechnology, that could be of wide interest. review on genetic transformation in citrus; micropropagation This special issue received 20 papers, of which 2 papers of Origanum acutidens (Hand.-Mazz.) Ietswaart using stem were withdrawn and 4 were rejected (not for their quality but node explants; use of tissue culture techniques for producing for their relatedness to the special issue). virus-free plant in garlic; adventitious shoot regeneration The guest editorial board of this issue would like to from leaf explant of aquatic plant Hygrophila polysperma mention that the published papers in this special issue contain (Roxb.) T. Anderson); secondary somatic embryogenesis in one paper on molecular and genetic diversity from China that Hovenia dulcis Thunb.; effects of IAA, IBA, NAA, and GA3 studied 115 sugarcane genotypes based on five genomic simple on rooting and morphological features of Melissa officinalis sequence repeat marker (gSSR) loci and 88 polymorphic alle- L. stem cuttings; comparison of different methods for sepa- lesoflociasdetectedbycapillaryelectrophoresis.Theresults ration of haploid embryo induced through irradiated pollen indicated large intrapopulation genetic variation compared and their economic analysis in melon (Cucumis melo var. with interpopulation variation. The knowledge obtained in inodorus); and comparative studies on cellular behaviour this study should be useful for future breeding programs. of carnation (Dianthus caryophyllus Linn. Cv. Grenadin) One paper discusses the problem of mantled fruits grown in vivo and in vitro for early detection of somaclonal as a result of somaclonal variation in oil palm plantlets variations and the influence of 1-triacontanol on the growth, regenerated via tissue culture. The molecular aspects of the flowering, and quality of potted Bougainvillea glabra var. occurrence possibly due to gene repression such as DNA “Elizabeth Angus” under natural conditions. Still in another methylation, histone methylation, and histone deacetylation paper published in this issue, there is important information are discussed. This paper described the total protein poly- about “Annotation of differentially expressed genes in the morphism profiles of somaclonal variants of oil palm and the somatic embryogenesis of Musa and their location in the effects of histone deacetylation on this phenomenon. banana genome.” The important information about TDFs 2 The Scientific World Journal sequences opens new possibilities for an in-depth study on molecularandbiochemicalresearchpertainingtozygoticand somatic embryogenesis in Musa species. These highly esteemed papers are easily available in the form of open access papers at the website of the jour- nal (http://www.hindawi.com/journals/tswj/psi/biotechnol- ogy/). The journal is indexed in many important international databases including Science Citation Index Expanded, Zoo- logical Record, and BIOSIS Previews. Acknowledgments The editors thank the authors and the management of the journal for their continued interest, that helped the guest editors to maintain the highest ethical standards to ensure publication of high-quality nonplagiarised original scientific material in the journal. It has all been possible due to the rigorous labour, hard work, dedication, and team work at each end. Khalid Mahmood Khawar Selma Onarici Muhammad Aasim Abdul Qayyum Rao Cigdem Alev Ozel Allah Bakhsh Hindawi Publishing Corporation The Scientific World Journal Volume 2013, Article ID 608650, 6 pages http://dx.doi.org/10.1155/2013/608650 Research Article A Comparison of Ice Cold Water Pretreatment and -Bromonaphthalene Cytogenetic Method for Identification of Papaver Species Amir Rezaei Osalou,1 Sheida Daneshvar