Protistology 10 (1), 26–42 (2016) Protistology Culture collection of parasitic protists at the Zoological Institute RAS (CCPP ZIN RAS) Marina N. Malysheva1, Maria A. Mamkaeva2, Alexei Yu. Kostygov1,3, Alexander O. Frolov1 and Sergey A. Karpov1,2 1 Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russia 2 St. Petersburg State University, St. Petersburg, Russia 3 Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic Summary Establishment of the bank of living cultures of trypanosomatids at the Laboratory of Protozoology, Zoological Institute of the Russian Academy of Sciences, St. Petersbug, started in 1979. The first edition of the collection catalogue (Malysheva et al., 2001) included 33 strains of trypanosomatids. The present, second edition contains descriptions of 52 strains of trypanosomatids and 28 strains of aphelids and parasitic chytridiomycetes. The strains of aphelids and parasitic chytridiomycetes were isolated and initially cultivated at the Center for Culturing Collection of Microorganisms of the Research park of St. Petersburg State University. This collection is named the Culture Collection of Parasitic Protists of the Zoological Institute RAS (CCPP ZIN RAS). Key words: Aphelididae, bibliography, culture collection, GenBank, Gromochytri- aceae, living cultures, Mesochytriaceae, parasitic protists, Trypanosomatidae Introduction depositarium of parasitic protozoans was established at the Laboratory of Protozoology of the Zoological Study of biodiversity of parasitic protists is Institute RAS in 1979. The first edition of the one of the “hot spots” in modern biology. Many collection catalogue was published in 2001 and of these organisms cause dangerous infections of included 33 strains of trypanosomatids (Malysheva humans, animals and plants, and their genetic et al., 2001). The present, second edition contains peculiarities are the unique examples of evolutionary descriptions of 52 strains of trypanosomatids changes in eukaryotes. The establishment, support augmented with 28 strains of aphelids and parasitic and accession of such culture collections are chytridiomycetes. The structure of the catalogue is very important tasks for study of biodiversity, generally the same as in the first edition (Malysheva cell biology, and parasite-host interactions. A et al., 2001); however, it was modified in two aspects: © 2016 The Author(s) Protistology © 2016 Protozoological Society Affiliated with RAS Protistology · 27 1) the deposition GenBank Numbers were included, Host: Ornidia obesa (Fabricius, 1775) (Diptera). and 2) a new section “List of the lost strains” was GenBank: AF038023, AF038026, EU079130, added. EU095978, HM593012 Bibliography: Fiorini et al., 1989; Faria e Silva Cultivating methods et al., 1991; Hollar et al., 1998; Yurchenko et al., 2009, Teixeira et al., 2011. Axenic trypanosomatid cultures have been maintained in the liquid nutrient medium BHI Genus Crithidia Léger, 1902 (Difco) with addition of hemin (25 mg/ml) and C. brevicula Frolov et Malysheva, 1989 inoculated once a month. The cultures were placed Synonyms: Crithidia allae Frolov et Malysheva, in the fridge (5° C) after inoculation and subsequent 1989 (NBrA); Wallaceina brevicula Frolov et incubation during three days at 25° C. Malysheva, 1989 (NBr, NBrB); Wallaceina incons- For cultivation of the Blastocrithidia flagellates, tans Podlipaev, Frolov et Kolesnikov, 1990 (GK); a two-phase medium has been used: the blood agar as Wallaceina vicina Malysheva et Frolov, 2004 (WG); a dense phase, and a BHI medium with the addition Leptomonas peterhoffi Podlipaev, 1985 (in part) of serum (5-10%) as a liquid phase. For cultivation (101); Wallaceina podlipaevi Malysheva et Frolov, of the Trypanosoma flagellates, a slightly modified 2009 (101); Blastocrithidia gerricola Podlipaev, 1985 two-phase medium has been used: the blood agar as (in part) (KV1); Blastocrithidia miridarum Podlipaev a dense phase, and a physiological solution with the et Frolov, 1987 (in part) (BM33); Leptomonas addition of serum (5-10%) as a liquid phase. sp. (F2); Leptomonas sp. (F5); Leptomonas sp. Accumulating cultures of the aphelids and (F6); Leptomonas sp. (F7); Leptomonas sp. (F8); chytrids were maintained in culture on Tribonema Leptomonas sp. (CL8). gayanum Pasch. (strain 20 CALU) as the host 2 Strain NBr cultivated in the mineral medium: K2HPO4 (66.7 Depositor: A.O. Frolov mg/l); MgSO4 × 7H2O (33 mg/l); KNO3 (100 History: 1986, isolated by A.O. Frolov in the mg/l); microelements (1 mg/l) of stock solution: vicinity of the Lyady village, Pskov Province, Russia. ZnSO4 × 7H2O (0.22 g/l), MgSO4 (1.81 g/l), CuSO4 Host: Nabis (Nabis) brevis Scholtz, 1847 (Hete- × 5H2O (0.079 g/l), NaBO3 × 4H2O (2.63 mg/l), roptera: Nabidae). (NH4)6Mo7O2 × 4H2O (1 mg/l), FeSO4 × 7H2O (9.3 GenBank: AF153045, AF316620, AJ401357, mg/l), CaCl2 (1.2 mg/l), Co(NO3)2 × H2O (0.02 AJ401378, EU088277- EU088279, KJ443335- mg/l), EDTA (10 mg/l), pH 7.0-7.2 (Gromov and KJ443337, KJ443350, KJ474892- KJ474894, Titova, 1991) in the 12 ml glass tubes containing KJ474931, KJ474932, Z32854, 3-5 ml of the medium at room temperature in Bibliography: Frolov and Malysheva, 1989a, the presence of light (24 h). The inoculation was 1989b; 1990; Podlipaev et al., 1991; Malysheva and performed twice a month. Frolov, 1995; Podlipaev et al., 1998; Podlipaev and Frolov, 2000; Yurchenko et al., 2000; Merzlyak Catalogue of living cultures et al., 2001a, 2001b; Yurchenko et al., 2006; Yurchenko et al., 2009; Kostygov et al., 2014 Class Kinetoplastea Honigberg 1963 emend. 3 Strain NBrB Vickerman 1976 Depositor: A.O. Frolov Subclass Metakinetoplastina Vickerman 2004 History: Clonal culture. 1986, isolated from Order Trypanosomatida Kent 1880 stat. nov. the culture of C. brevicula (NBr) in the Laboratory Hollande 1952 of Protozoology ZIN RAS, St. Petersburg, Russia. Family Trypanosomatidae Doflein 1951 Host: Nabis (Nabis) brevis Scholtz, 1847 (Hete- Genus Angomonas Souza and Corte-Real, 1991 roptera: Nabidae). A. deanei (Carvalho, 1973) Bibliography: Frolov and Malysheva, 1989a, Synonyms: Crithidia deanei Carvalho, 1973; 1989b; Kolesnikov et al., 1990; Skarlato et al., 1990; Crithidia roitmani, Fiorini et al., 1989; Herpetomonas Podlipaev et al., 1991; Malysheva and Frolov, 1995. roitmani (Fiorini et al., 1989) 4 Strain WG 1 Strain TCC080E (=ATCC 50305) Depositor: M.N. Malysheva Depositor: S.A. Podlipaev History: 2000, isolated by M.N. Malysheva in History: 1990, received from E.P. Camargo, São Komarovo, Leningrad Province, Russia. Paulo University, Brazil. Isolated in Brazil. Host: Limnoporus rufoscutellatus (Latreille, 28 · Marina N. Malysheva et al. 1807) (Heteroptera: Gerridae). Host: Nabis (Nabicula) flavomarginatus Scholtz, GenBank: KJ443338-KJ443340, KJ443354, 1847 (Heteroptera: Nabidae). KJ474895-KJ474897, KJ474907-KJ474909 GenBank: AF375664, DQ140170, KJ443317- Bibliography: Kostygov et al., 2004; Malysheva KJ443319, KJ443344, KJ474874-KJ474876, and Frolov, 2004; Kostygov et al., 2014. KJ474913-KJ474915, 5 Strain 101 Bibliography: Podlipaev and Rokitskaya, 1999; Depositor: S.A. Podlipaev Kostygov et al., 2004; Yurchenko et al., 2006; History: 1980, isolated by S.A. Podlipaev in Kostygov et al., 2014. Peterhof, St. Petersburg, Russia. 9 Strain F5 Host: Nabis (Nabicula) flavomarginatus Scholtz, Depositor: S.A. Podlipaev 1847 (Heteroptera: Nabidae). History: 1986, isolated by S.A. Podlipaev at the GenBank: JN944133, AF153039, AF322390, Cape Kartesh (White Sea), Karelia, Russia. EU088283-EU088285, KJ443308-KJ443311, Host: Nabis (Nabicula) flavomarginatus Scholtz, KJ474867, KJ474935-KJ474937, KJ443306, 1847 (Heteroptera: Nabidae). KJ443307, KJ474866, KJ474933, KJ474934 GenBank: AY547460, KJ443320-KJ443322, Bibliography: Podlipaev, 1985; Kolesnikov et KJ443345, KJ474877-KJ474879, KJ474923, al., 1990; Marakhova et al., 1991; Podlipaev et al., KJ474924 1991; Podlipaev et al., 1998; Podlipaev and Frolov, Bibliography: Podlipaev et al., 1991; Podlipaev 2000; Merzlyak et al., 2001a; Kostygov et al., 2004; and Rokitskaya, 1999; Kostygov et al., 2004; Yurchenko et al., 2006; Malysheva and Frolov, Podlipaev et al., 2004; Kostygov et al., 2014. 2009; Yurchenko et al., 2009; Gerasimov et al., 10 Strain F7 2012a; Kostygov et al., 2014. Depositor: S.A. Podlipaev 6 Strain KV1 History: 1986, isolated by S.A. Podlipaev at the Synonyms: Blastocrithidia gerricola Podlipaev, Cape Kartesh (White Sea), Karelia, Russia. 1985 (in part). Host: Nabis (Nabicula) flavomarginatus Scholtz, Depositor: S.A. Podlipaev 1847 (Heteroptera: Nabidae). History: 1981, isolated by S.A. Podlipaev at the GenBank: KJ443326-KJ443328, KJ443347, Curonian Spit, Kaliningrad Province, Russia. KJ474883-KJ474885, KJ474910-KJ474912 Host: Gerris lacustris (Linnaeus, 1758) (Hete- Bibliography: Kostygov et al., 2004; Kostygov roptera). et al., 2014. GenBank: AF153036, AF322391, AJ401379, 11 Strain CL8 AY547459; KJ443332-KJ443334, KJ443351, Depositor: A.O. Frolov KJ474889-KJ474891, KJ474928-KJ474930. History: 1984, isolated by A.O. Frolov in Bibliography: Podlipaev, 1985; Kolesnikov et Peterhof, St. Petersburg, Russia. al., 1990; Podlipaev et al., 1991; Podlipaev et al., Host: Nabis (Dolichonabis) limbatus Dahlbom, 1998; Podlipaev and Frolov, 2000; Merzlyak et al., 1851 (Heteroptera: Nabidae). 2001a, 2001b; Podlipaev et al., 2004; Yurchenko et GenBank: KJ443314-KJ443316, KJ443349, al., 2006; Kostygov et al., 2014. KJ474871-KJ474873, KJ474925-KJ474927 7 Strain BM33 Bibliography: Podlipaev and Rokitskaya, 1999; Depositor: A.O.