10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Integrin beta 7 Polyclonal Antibody Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP54281 Specification Integrin beta 7 Polyclonal Antibody - Product Information Application IHC-P Primary Accession P26011 Reactivity Rat Host Rabbit Clonality Polyclonal Calculated MW 87411 Integrin beta 7 Polyclonal Antibody - Additional Information Gene ID 16421 Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and Other Names paraffin-embedded; Integrin beta-7, Integrin beta-P, M290 IEL Antigen retrieval: citrate buffer ( 0.01M, pH antigen, Itgb7 6.0 ), Boiling bathing for 15min; Block Format endogenous peroxidase by 3% Hydrogen 0.01M TBS(pH7.4) with 1% BSA, 0.09% peroxide for 30min; Blocking buffer (normal (W/V) sodium azide and 50% Glyce goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Integrin beta 7 Polyclonal Storage Antibody, Unconjugated(bs-1051R) 1:200, Store at -20 ℃ for one year. Avoid repeated overnight at 4°C, followed by conjugation to freeze/thaw cycles. When reconstituted in the secondary antibody(SP-0023) and sterile pH 7.4 0.01M PBS or diluent of DAB(C-0010) staining antibody the antibody is stable for at least two weeks at 2-4 ℃. Integrin beta 7 Polyclonal Antibody - Protein Information Name Itgb7 Function Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is involved in adhesive interactions of leukocytes. It is a receptor for fibronectin and recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1 and VCAM1. It recognizes the sequence L-D-T in MADCAM1. Integrin alpha-E/beta-7 is a Page 1/2 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 receptor for E- cadherin. Cellular Location Membrane; Single-pass type I membrane protein. Integrin beta 7 Polyclonal Antibody - Protocols Provided below are standard protocols that you may find useful for product applications. • Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture Page 2/2 Powered by TCPDF (www.tcpdf.org).