US007795.503B2 (12) United States Patent (10) Patent No.: US 7,795,503 B2 Apuya et al. (45) Date of Patent: Sep. 14, 2010 (54) MODULATING PLANT ALKALOIDS 6,013,863 A 1/2000 Lundquist et al. 6,087,558 A 7/2000 Howard et al. (75) Inventors: Nestor Apuya, Culver City, CA (US); 6,093,874 A 7/2000 Jofuku et al. Steven Craig Bobzin, Malibu, CA (US); 6,136,320 A 10/2000 Arntzen et al. Joon-Hyun Park, Oak Park, CA (US) 6,235,975 B1 5, 2001 Harada et al. 6.255,562 B1 7/2001 Heyer et al. (73) Assignee: Ceres, Inc., Thousand Oaks, CA (US) 6,271,016 B1 8/2001 Anderson et al. 6,294,717 B1 9, 2001 Xie (*) Notice: Subject to any disclaimer, the term of this 6,303,341. B1 102001 Hiatt et al. patent is extended or adjusted under 35 6,326,527 B1 12/2001 Kirihara et al. U.S.C. 154(b) by 824 days. 6,329,567 B1 12/2001 Jofuku et al. 6,329,571 B1 12/2001 Hiei (21) Appl. No.: 11/360,459 6,417,429 B1 7/2002 Hein et al. 6,423,885 B1 7/2002 Waterhouse et al. (22) Filed: Feb. 22, 2006 6,452,067 B1 9, 2002 Bedbrook et al. 6,518,066 B1 2/2003 Oulmassov et al. (65) Prior Publication Data 6,573,099 B2 6/2003 Graham 6,645,765 B1 1 1/2003 Anderson et al. US 2006/O195934 A1 Aug. 31, 2006 6,664,446 B2 12/2003 Heard et al. 6,706,470 B2 3/2004 Choo et al. Related U.S. Application Data 6,753,139 B1 6/2004 Baulcombe et al. (60) Provisional application No. 60/654,927, filed on Feb. 6,777,588 B2 8, 2004 Waterhouse et al. 6,835,540 B2 12/2004 Broun 22, 2005. 6,846,669 B1 1/2005 Jofuku et al. 6,906.244 B2 6/2005 Fischer et al. (51) Int. Cl. - WW CI2N 15/29 (2006.01) SA, SSEord et al. CI2N 5/82 (2006.01) 2003/OO37355 A1 2/2003 Barbas et al. AOIH 5/00 (2006.01) 2003/006 1637 A1 3/2003 Jiang et al. (52) U.S. Cl. ....................... 800/298; 800/287: 800/312: 2003/0131386 A1 7/2003 Samaha et al. 800/317; 800/321 2003. O135887 A1 7, 2003 Brandle et al. (58) Field of Classification Search ....................... None 2003. O140381 A1 7, 2003 Bate et al. 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(74) Attorney, Agent, or Firm Fish & Richardson P.C. 5,445,934 A 8, 1995 Fodor et al. 5,538,880 A 7, 1996 Lundquist et al. (57) ABSTRACT 5,723,766 A 3/1998 Theologis et al. 5,766,847 A 6, 1998 Jackle et al. 5,777,079 A 7, 1998 Tsien et al. Materials and methods are provided for identifying regula 5,824,779 A 10/1998 Koegel et al. tory region-regulatory protein associations and modulating 5,824.798. A 10, 1998 Tallberg et al. expression of a sequence of interest. 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