FISHERIES RESEARCH BOARD OF CANADA Translation Series No. 1942 Influence of sodium selenite, sodium tellurite and sodium sulfite on the retention and distribution of cadmium in mice by V. Eybl, J. Sykora and F. Mertl Original title: Einfllivon natriumselenit, natriumtellurit and natriumsulfit auf retention und verteilung von cadmium bei mausen From: Archiv fur toxikologie, (Archive of Toxicology), 26: 169-175, 1970 • Translated by the Translation Bureau(MV) Foreign Languages Division Department of the Secretary of State of Canada Fisheries Research Board of Canada Halifax Laboratory Halifax, N. S. 1971 11 pages typescript rdiva DEPARTMENT OF THE SECRETARY OF STATE SECRÉTARIAT D'ÉTAT V.7 TRANSLATION BUREAU BUREAU DES TRADUCTIONS FOREIGN LANGUAGES DIVISION DES LANGUES DIVISION CANADA ÉTRANGÈRES il TRANSLATED FROM - TRADUCTION DE INTO - EN German English AUTHOR - AUTEUR EYBL, V., et al. TITLE IN ENGLISH - TITRE ANGLAIS Influence of sodium selenite, sodium tellurite and sodium sulfite on the ret'ention and distribution of cadmium in mice Title in foreign language (traasliterate foreign -charaeters) Einflup von Natriumselenit, Natriumtellurit und Natriumsulfit auf Retention und Verteilung von Cadmium bei Mâusen RVF5RENCE IN FOREIGN I,ANGUnE (NAME OF BOOK OR PUBLICATION) IN FULL. TRANSLITERATE FOREIGN CHAFACTERS. • REFE.RENCE EN LANGUE ETRANGERE (NOM DU LIVRE OU PUBLICATION), AU COMPLET. TRANSCRIRE EN CARACTERES PHONETIQUES. .Archiv für Toxikologie REFEREN CE IN ENGLISH - RÉFÉRENCE EN ANGLAIS Archive of Toxicology PUBLISH ER - EDITEUR PAGE NUMBERS IN ORIGINAL DATE OF PUBLICATION NUMÉROS DES PAGES DANS not stated DATE DE PUBLICATION L'ORIGINAL YEAR ISSUE.NO . 169-175 . VOLUME ANNÉE NUMERO PLACE OF PUBLICATION . NUMBER OF TYPED PAGES LIEU DE PUBLICATION • NOMIRE DE PAG„ES DACTYLOGRAPHIEES Germany 1970 11 TRANSLATION BUREAU NO. REQUEÏTING DEPARTMENT Fisheries & Forestry 1133 MIN ISTERE-CLIENT NOTRE DOSSIER NO BRANCH OR DIVISION Fisheries Research Board TRANSLATOR (INITIALS) MV DIRECTION OU DIVISION TRADUCTEUR (INITIALES) PERSON ftEQUESTING DATE C,OMPLETED 3.12.71 'DEMANCIE PAR • Mr. H.C. Freeman, Halifax ACHEVE LE • Laboratory YOUR NUMBER 769-18"-14 VOTRE DOSSIER N° UNSDITÈD TRANSLAtet4 DATE OF REQUEST 8.11.71 DATE DE LA DEMANDE Pe infseutW *Ai TRADUCTION NON IkIVISEt Infonnition soultmeet $ 05.200-10-0 (REV. 2/ 138) ' DEPARTMENT OF THE SECRETARY OF STATE SECRÉTARIAT D'ÉTAT TRANSLATION BUREAU BUREAU DES TRADUCTIONS ' FOREIGN LANGUAGES DIVISION DIVISION DES LANGUES ÉTRANGÈRES CANADA CLIENTS NO. DEPARTMENT DIVISION/BRANCH CITY No DU CLIENT MINISTERE DIVISION/DIRECTION VILLE 769-18-14 Fisheries & Forestry Fisheries Research Board Halifax , N. s . BUREAU NO. LANGUAGE TRANSLATOR (INITIALS) DATE No DU BUREAU LANGUE TRADUCTEUR (INITIALES) 1133 German MV Archiv für Toxikologie, Volume 26, pp.169-175, 1970 Short Communication Influence of sodium selenite, sodium tellurite and 169 sodium sulfite on the retention and distribution of cadmium •in mice By V. EYBL, J. SÙORA and F. MERTL Pharmacological Institute and Institute of Medical Physics, Faculty of Medicine, Karls University, Pilsen, CSSR (Received October 17, 1969) Summary Summary. The retention and distribution of cadmium in the organism during a 4 week period after i.v. administration of 115 inedll (%sith earrier). as well as the influence of s.c. application of sodium selenite. sodiurn tellurite and sixlium sulfite on this retention and distribution were studied in experiments on mire. Sodium selenite was found to cause a prolonged retention of cadmium in the organism. All of these compounds alter corumiderably the distribution of cadmium in th.e organs. Key-11.°We Cadmium — Selenite — Sulfite — Tellurite Traee Elernents. UNEDITED TRANSLATION For Information only TRACIUCTION NON REVUE Ihfcletiièfien SOS-200-10-31 ".‘ 2 In previous publications (EYBL et al., 1968, 1969a) we reported that sodium selenite and sodium tellurite increase the retention of mercury in the body and alter the distribution of that element in several organs. Selenium acts as a protective agent in cadmium necrosis of the testes (GUNN et al., 1968; KAR et al., 1960, 1966; MASON et al., 1964), sodium selenite in /. cadmium and mercury poisoning (PedZEK et al., 1967, 1968; PARIZEK & OMV/£DALOVÂ, 1967). In these tests, too, changes in the distribution of the metals used were observed. On the other hand we know that cadmium and mercury increase the retention of selenium by the body 170 and affect the selenium metabolism (EYBL et al., 1969b; GANTHER & BAUMANN, 1962). The present study compares the influence of sodium selenite on the retention and distribution of cadmium in the body with that of the chemically related compounds of sodium tellurite and sodium sulfite, and examines the re-distribution of cadmium in relation to time. Methods Male mice (strain H, supplied by Velaz,.Prague) weighing 18-20 g were.used as test animals. They were divided into several .groups of 24 animals each. The first group received 3 x 10 -3 mM/kg ïee.Mià 3 115m of CdC1 2 (with carrier, specific activity 0.5 mCi/1 mg Cd; from The Radiochemical Centre, Amersham) intravenously, the other groups received the same dose, together with the subcutaneous ad- ministration of either sodium selenite, sodium tellurite or sodium sulfite. These substances (analytical grade, from Lachema) were dissolved in redistilled water and administered in doses of 3 x 10 -3 mM/kg (injection volume 0.1 m1/10 g). The 115mCdC.1 2 activity was approx. 3 uCi per Mouse. Cadmium retention was determined by total body measurements with a scintillation spectrometer immediately after administration, and 24 hours,as well as on the 7th, 14th and 21st day after admini- stration. The channel width was adjusted to •the total spectrum of the bremsstrahlung produced in the test animais by interaction with the high-energy (1.61 MeV) p-particles (LIDEN, 1958; MEHL, 1957). 24 hours and on the 7th and 28th day after administration, 8 mice from each group were sacrificed. They were decapitated, their blood was collected, and the following organs were removed: brain, heart, lungs, liver, digestive tract, kidneys, spleen, femora, and testes. As in the 'previous studies (EYBL et al., 1965), the organs, the blood and the remains of the mice were dried at 90 °C before the activity was measured with a Geiger-Müller counter. The results were corrected for the self-absorption of the p-particles. All results were expressed in percent of the anplied dose. 4 In the Figures the mean values are given with a 95% confidence interval. Results 1. Retention of cadmium by the body The influence of the various administered substances on the retention of cadmium in the body is compared in Fig.l. We see too ■ , ------- 90 •••■■._ ••■•• ••■■ Sg. -- • ....... ..... ; 14 21 28 7aavsw, - Fi .1. Cadmium retention in mice at various inter- vais after administration of the following doses: • 11 5mCdC12 iv. (control test) 115 mCdC1 2 iv. + Na2Se03 sc. 115mCdC1 2 iv. + Na 2Te03 sc. °-'•") 115mCdC12 iv. + Na 2 30 3 sc. expressed in percent of the total administered dose * significant difference from control value (P < 0.05) 5 that cadmium retention is greatest after the administration of sodium selenite, while sodium tellurite and sodium sulfite do not affect it significantly. The effect of sodium selenite is of long duration, for even on the 28th day 83% of the total administered dose of cadmium is retained in the body as a result of the influence of sodium selenite, while in a control test over the same time interval the retention is 75%. 2. Cadmium content in - organs 171 The cadmium contents found in the organs at different inter- vals after administration are shown in F.igs. 2 and 3. a) Influence of sodium selenite. On account of the influence of sodium selenite, 24 hours after administration the cadmium con- tent in the liver is higher than in the controls, but it subsequently rises in both groups and becomes the same. In the blood, too, the cadmium content is increased after 24 hours, but it decreases in the course of time. In the spleen the cadmium content is increased in the 1st week, in the testes and in the remains of the mice in the 4th week. In the kidneys the cadmium content is lower than in the controls during the first 24 hours, then increases sharply, and is higher than in the control group in the 4th week. The cadmium content in heart, lungs and femora decreases in the first 24 hours, in the brain during the first 24 hours and in the 1st week. 50 - 40 30 20 10 • • 40 30 20 10 o Fig.2. Influence of Na2Se0 3 , Na2Te0 3 and Na-SO 3 on the cadmium contents in the organs of mice at different intervals after administration (expressed in percent of the total administered dose; mean values with a 95% confidence interval). L - liver V - digestive tract N - kidneys 1:1 - remains h) Influence of sodium tellurite. - After 24 hours the cadmium content in the liver is higher than in the controlà, but . subsequently it drops to the content found in the control group. 1_72 7 24 9d hrs. actio2 CdCi2 • ` k125P0) to T Cd02 tk1 2 TPO CACl2 .01'12 1;0 3 Fig.3. Influence of Na 2 Se03 , Na 2 Te0 3 and Na2 50 3 on the.cadmium contents in the organs of mice at different intervals after administration (expressed in percent of the total administered dose: mean values with a 95% confidence interval). M - spleen H - heart L - lungs G - brain 0 - femora Ho - testes B - blood This increase during the first 24 hours is greater than after the administration of sodium selenite. The cadmium contents of kidneys and blood are decreased after , 24 hours. In the digestive tract the cadmium content is increased in the 4th week; in the brain it is decreased after 24 hours and in the 1st week, also in the remains of the mice and in the heart fter 24 hours and in the 4th week.