SAR1B Antibody Cat. No.: 55-163 SAR1B Antibody Flow cytometric analysis of 293 cells (right histogram) Western blot analysis in MCF-7,MDA-MB453 cell line compared to a negative control cell (left histogram).FITC- lysates (35ug/lane). conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Mouse, Pig, HOMOLOGY: Rat This SAR1B antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 89-116 amino acids from the Central region of human SAR1B. TESTED APPLICATIONS: Flow, IHC-P, WB September 26, 2021 1 https://www.prosci-inc.com/sar1b-antibody-55-163.html For IHC-P starting dilution is: 1:50~100 APPLICATIONS: For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 PREDICTED MOLECULAR 22 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: SAR1B ALTERNATE NAMES: GTP-binding protein SAR1b, GTP-binding protein B, GTBPB, SAR1B, SARA2, SARB ACCESSION NO.: Q9Y6B6 PROTEIN GI NO.: 14285769 GENE ID: 51128 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References The protein encoded by this gene is a small GTPase that acts as a homodimer. The encoded protein is activated by the guanine nucleotide exchange factor PREB and is involved in protein transport from the endoplasmic reticulum to the Golgi. This protein is BACKGROUND: part of the COPII coat complex. Defects in this gene are a cause of chylomicron retention disease (CMRD), also known as Anderson disease (ANDD). Two transcript variants encoding the same protein have been found for this gene. September 26, 2021 2 https://www.prosci-inc.com/sar1b-antibody-55-163.html REFERENCES: 1) Cefalu, A.B., et al. Metab. Clin. Exp. 59(4):463-467(2010) 2) Silvain, M., et al. Clin. Genet. 74(6):546-552(2008) 3) Charcosset, M., et al. Mol. Genet. Metab. 93(1):74-84(2008) 4) Jardim, D.L., et al. J. Biochem. Mol. Biol. 38(3):328-333(2005) ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 3 https://www.prosci-inc.com/sar1b-antibody-55-163.html.