Anti-OTUB1 monoclonal antibody, clone FQS24138(C) (DCABH-6470) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Product Overview Rabbit monoclonal to OTUB1 Antigen Description Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128- mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of Lys-48-linked polyubiquitin chains, but not Lys-63-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to Lys- 48-linked ubiquitin. Immunogen Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human OTUB1 aa 50-150 (Cysteine residue). The exact sequence is proprietary.Database link: Q96FW1 Isotype IgG Source/Host Rabbit Species Reactivity Mouse, Rat, Human Clone FQS24138(C) Purity Tissue culture supernatant Conjugate Unconjugated Applications WB Positive Control MCF7, HeLa, HepG2 and 293T cell lysates. Format Liquid Size 100 μl 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Preservative None Storage Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Ship Shipped at 4°C. GENE INFORMATION Gene Name OTUB1 OTU domain, ubiquitin aldehyde binding 1 [ Homo sapiens ] Official Symbol OTUB1 Synonyms OTUB1; OTU domain, ubiquitin aldehyde binding 1; ubiquitin thioesterase OTUB1; FLJ20113; FLJ40710; otubain-1; OTU-domain Ubal-binding 1; deubiquitinating enzyme OTUB1; ubiquitin- specific protease otubain 1; ubiquitin-specific-processing protease OTUB1; OT Entrez Gene ID 55611 Protein Refseq NP_060140 UniProt ID B3KUV5 Chromosome Location 11q13.1 Function NEDD8-specific protease activity; cysteine-type peptidase activity; omega peptidase activity; peptidase activity; protein binding; ubiquitin binding; ubiquitin-specific protease activity; 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.