US 20160286803A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2016/0286803 A1 HELLWEGE et al. (43) Pub. Date: Oct. 6, 2016 (54) ACTIVE COMPOUNDS COMBINATION Publication Classification CONTAINING FILUOPYRAM BACILLUS AND BOLOGICALLY CONTROL AGENT (51) Int. Cl. AOIN 43/40 (2006.01) (71) Applicants: BAYER INTELLECTUAL AOIN 63/04 (2006.01) PROPERTY GMBH, Monheim (DE); AOIN 63/00 (2006.01) BAYER CROPSCIENCE AG, (52) U.S. Cl. Monheim (DE) CPC ............... A0IN 43/40 (2013.01): A0IN 63/00 (2013.01); A0IN 63/04 (2013.01) (72) Inventors: Elke HELLWEGE, Langenfeld (DE): (57) ABSTRACT Heike HUNGENBERG, Langenfeld The present invention relates to active compound combina (DE) tions for reducing the damage of plants and plant parts as Assignees: BAYER INTELLECTUAL well as losses in harvested fruits or vegetables caused by (73) insects, nematodes or phytopathogens and which have fun PROPERTY GMBH; BAYER gicidal or nematicidal or insecticidal activity including any CROPSCIENCE AG combination of the three activities, in particular within a composition, which comprises (A) Fluopyram, (B) a spore (21) Appl. No.: 15/175,810 forming bacterium of the genera Bacillus, selected from Bacillus firmus, Bacillus cereus, Bacillus pumilis, Bacillus amyloliquefaciens, Bacillus subtilis strain GB03, Bacillus (22) Filed: Jun. 7, 2016 subtilis strain QST713, and (C) at least one biological control agent, in particular bacteria, fungi or yeasts, proto Zoa, viruses, entomopathogenic nematodes, inoculants, Related U.S. Application Data botanicals and products produced by microorganisms (62) Division of application No. 14/373.916, filed on Jul. including proteins or secondary metabolites. Moreover, the 23, 2014, filed as application No. PCT/EP2013/ invention relates to a method for curatively or preventively 051108 on Jan. 22, 2013. controlling insects, nematodes or phytopathogens on the plant, plant parts, harvested fruits or vegetables, to the use (30) Foreign Application Priority Data of a combination according to the invention for the treatment of seed, to a method for protecting a seed and not at least to Jan. 25, 2012 (EP) .................................. 12152488.8 the treated seed. US 2016/0286803 A1 Oct. 6, 2016 ACTIVE COMPOUNDS COMBINATION 0008 Fluopyram and its manufacturing process starting CONTAINING FLUOPYRAM BACILLUS AND from known and commercially available compounds is BIOLOGICALLY CONTROL AGENT described in EP-A 1531 673 and WO 2004/O16088. 0009. A general description of the nematicidal activity of CROSS REFERENCE TO RELATED pyridylethylbenzamide derivatives is found in WO-A 2008/ APPLICATIONS 126922. A description of nematicidal activity of Bacteria, in particular Bacillus firmus is found in WO-A 1996/32840, a 0001. This application is a divisional application of Ser. description of nematicidal activity of Bacillus chitinosporus No. 14/373,916 (filed Jul. 23, 2014), which is a S371 is found in WO-A 982 1966. National Stage Application of PCT/EP2013/051108 (filed 0010. The use of bacteria such as Bacillus sp. as biologi Jan. 22, 2013), which claims priority to EP 12152488.8 cal control agent in Synergistic compositions with fungicides (filed Jan. 25, 2012), the contents of each of which are e.g. Fluopyram for controlling phytopathogenic organisms incorporated herein by reference in their entirety. in agriculture is described e.g. in US 2011/0110906 A, US 2010/0249193 A, US 20100/209410 A, EP 2460407 A and BACKGROUND WO 2012/016989. In these documents only binary mixtures 0002 1. Field of the Invention of the biological control agent and Fluopyram are disclosed. 0003. The present invention relates to active compound WO 2012/038480 A discloses seeds of a plant comprising combinations and compostions for reducing the damage of (a) a gene preferably AXmi031, and AXn2 (producing pro plants and plant parts as well as losses in harvested fruits or teins of Bacillus thuringiensis), (b) a biological control vegetables caused by insects, nematodes or phytopathogens agent preferably Bacillus firmus CNCM I-1582, (c) one or and which have fungicidal or nematicidal or insecticidal more insecticides (including biological control agents pref activity including any combination of the three activities, in erably Pasteuria and Verticillum and (d) one or more fun particular within a composition, which comprises (A) Flu gicides preferably Fluopyram. In this document Pasteuria opyram, (B) a spore-forming bacterium of the genera Bacil and Verticillium are not further specified. lus, selected from Bacillus firmus, Bacillus cereus, Bacillus 0011 Since the environmental and economic require pumilis, Bacillus amyloliquefaciens, Bacillus subtilis strain ments imposed on modern-day crop protection compositions GB03, Bacillus subtilis strain QST713, and (C) at least one are continually increasing, with regard, for example, to the biological control agent, in particular bacteria, fungi or spectrum of action, toxicity, selectivity, application rate, yeasts, protozoa, Viruses, entomopathogenic nematodes, formation of residues, and favourable preparation ability, inoculants, botanicals and products produced by microor and since, furthermore, there may be problems, for example, ganisms including proteins or secondary metabolites. More with resistances, a constant task is to develop new compo over, the invention relates to a method for curatively or sitions, in particular fungicidal or nematicidal agents, which preventively controlling insects, nematodes or phytopatho in some areas at least help to fulfil the abovementioned gens on the plant, plant parts, harvested fruits or vegetables, requirements. The present invention provides active com to the use of a combination according to the invention for the pound combinations/compositions which in Some aspects at treatment of seed, to a method for protecting a seed and not least achieve the stated objective. at least to the treated seed. 0004 2. Description of Related Art SUMMARY 0005 Fluopyram is defined to be the compound of the 0012. It has now been found, surprisingly, that the com formula (I) binations according to the invention not only bring about the additive enhancement of the spectrum of action with respect to the insects, nematodes or phytopathogens to be controlled (I) that was in principle to be expected but achieves a syner CF s1 C. gistic effect which extends the range of action of the component (A) and of the component (B) and of the com 2 ponent (C) in two ways. Firstly, the rates of application of N the component (A) and of the component (B) and of the component (C) are lowered whilst the action remains H equally good. Secondly, the combination still achieves a high degree of control of insects, nematodes or phytopatho 0006 as well as the N-oxides of the compound thereof. gens even where the two individual compounds have 0007 Fluopyram is a broad spectrum fungicide of the become totally ineffective in such a low application rate chemical class of pyridylethylbenzamide derivatives with range. This allows, on the one hand, a substantial broadening penetrant and translaminar properties for foliar, drip, drench of the spectrum of phytopathogenic fungi and nematodes and seed treatment applications on a wide range of different that can be controlled and, on the other hand, increased crops against many economically important plant diseases. safety in use. It is very effective in preventative applications against 0013. In addition to the fungicidal or nematicidal or powdery mildew species, grey mould and white mould insecticidal Synergistic activity, the active compound com species. It has an efficacy against many other plant diseases. binations according to the invention have further Surprising Fluopyram has shown activity in spore germination, germ properties which, in a wider sense, may also be called tube elongation and mycelium growth tests. At the bio synergistic, Such as, for example: broadening of the activity chemical level, fluopyram inhibits mitochondrial respiration spectrum to other insects, nematodes or phytopathogens, for by blocking the electron transport in the respiratory chain of example to resistant strains of plant diseases; lower appli Succinate Dehydrogenase (complex II-SDH inhibitor). cation rates of the active compound combination; Sufficient US 2016/0286803 A1 Oct. 6, 2016 control of pests with the aid of the active compound com pumilis, Bacillus amyloliquefaciens, Bacillus subtilis strain binations according to the invention even at application rates GB03, Bacillus subtilis strain QST713, and where the individual compounds show no or virtually no 0031 (C) at least one biological control agent selected activity; advantageous behaviour during formulation or dur from the group consisting of ing use, for example during grinding, sieving, emulsifying, 0032 (C1) bacteria, dissolving or dispensing; improved storage stability and 0033) (C2) fungi or yeasts, light stability; advantageous residue formation; improved 0034 (C3) protozoas, toxicological or ecotoxicological behaviour; improved prop 0035 (C4) viruses, erties of the plant so called plant physiology effects, for 0036 (C5) entomopathogenic nematodes, example better growth, increased harvest yields, a better 0037 (C6) inoculants, developed root system, a larger leaf area, greener leaves, 0038 (C7) botanicals, and (C8.1) Halpin (produced by stronger shoots, less seed required, lower phytotoxicity, Erwinia amylovora) for reducing overall damage of plants mobilization of the defence system of the plant, good and