A: Picornavirales 0.5 AA subs Thika virus AKH40285 Drosophila uncharacterized virus AKP18620 95|97 Kilifi Virus YP_009140560 Machany Virus (Dobs) KU754504 Unclassified Rosy apple aphid virus ABB89048 Picornavirales 95|86 Acyrthosiphon pisum virus NP_620557 TSA Clavigralla tomentosicollis GAJX01000318 TSA Euschistus heros GBER01001913 B: Dicistroviruses TSA Bactrocera dorsalis GAKP01021200 TSA Ceratitis capitata GAMC01006902 0.5 AA subs TSA Bactrocera latifrons GDHF01000396 TSA Bactrocera cucurbitae GBXI01004087 TSA Fopius arisanus GBYB01002971 98|94 Goose dicistrovirus ALV83314 Empeyrat Virus (Sdef) KU754505 TSA Teleopsis dalmanni GBBP01093735 Sequence from Drosophila kikkawai [SRR346732] Cricket paralysis virus AKA63263 42|37 Nilaparvata lugens C virus AIY53985 Drosophila C Virus NP 044945 TSA Pontastacus leptodactylus GAFS01001458 44|28 Aphid lethal paralysis virus AEH26191 99|97 TSA Medicago sativa GAFF01020061 Rhopalosiphum padi virus ABX74939 TSA Nilaparvata lugens GAYF01148415 Cripavirus TSA Agave tequilana GAHU01086141 Himetobi P virus BAA32553 99|99 TSA Phaseolus vulgaris GAMK01054259 20|54 Black queen cell virus ABS82427 99|100 Triatoma virus AAF00472 Plautia stali intestine virus BAA21898 0.99 Homalodisca coagulata virus-1 ABC55703 92|87 Ancient Northwest Territories cripavirus AIM55450 100|99 TSA Colobanthus quitensis GCIB01076644 Antarctic picorna-like virus 1 AKG93960 30|50 Formica exsecta virus 1 AHB62420 87|37 100|94 Israeli acute paralysis virus AEL12438 Kashmir bee virus AAP32283 Acute bee paralysis virus AF150629 TSA Linepithema humile EZ933808 Solenopsis invicta virus-1 AAU85375 99|99 TSA Conwentzia psociformis GAYH01015373 Aparavirus TSA Menopon gallinae GAWR01012511 TSA Zorotypus caudelli GAYA01006867 65|35 10|57 Taura syndrome virus AFO53688 Macrobrachium rosenbergii Taihu virus AEE02024 94|58 Mud crab dicistrovirus ADR72923 98|47 TSA Procotyla fluviatilis GAKZ01048307 91|57 Laverivirus AHA86926 TSA Schmidtea mediterranea GBEE01003387 TSA Galloisiana yuasai GAWN01024221 96|96 TSA Stigmatomma oregonense GAXR01027179 TSA Metallyticus splendidus GATB01027408 TSA Camptotheca acuminata GACF01095404 98|44 TSA Silene latifolia JO773273 TSA Euroleon nostras GAXW01089633 C: Nodaviruses Drosophila melanogaster American nodavirus ACU32794 100|87 Flock house virus AEQ39075 0.5 AA subs 4|24 99|67 TSA Stigmatomma oregonense GAXR01015452 Black beetle virus CAA26238 Alphanodavirus 0|25 Boolarra virus AF329080 96|76 99|100 Newington Virus (Dimm) KU754529 99|66 Bat nodavirus AGN73379 TSA Bactrocera cucurbitae GBXI01012869 96|100 97|100 TSA Ceratitis capitata GAMC01011587 Covert mortality nodavirus AIL48199 Macrobrachium rosenbergii nodavirus AAO60068 Penaeus vannamei nodavirus ADV74764 98|96 Lutzomyia nodavirus AKP18615 Dicentrarchus labrax betanodavirus AET07013 99|98 Senegalese sole Iberian betanodavirus ACX71271 87|64 75|19 0|13 Epinephelus tauvina nervous necrosis virus AF319555 43|28 Asian seabass Nervous Necrosis Virus AEK48160 86|91 Sevenband grouper nervous necrosis virus AKE78836 Betanodavirus 100|94 Japanese flounder nervous necrosis virus ACN58225 Golden pompano nervous necrosis virus AEK48150 90|90 Barfin flounder virus ACF36168 100|99 Tiger puffer nervous necrosis virus ABY87293 Striped Jack nervous necrosis virus BAB64329 TSA Nilaparvata lugens GANM01015802 81|49 Pariacoto virus AF171942 Alphanodavirus 97|73 Mosinovirus AIO11151 Lunovirus AKA58518 52|50 Fesavirus 4 AII82234 97|77 Tetnovirus 2 ADK97710 69|22 TSA Opisthorchis felineus GBJA01011878 Tetnovirus 1 ADK97708 9|24 Craigies Hill virus (Dmel) AKH40302 62|62 Craigmillar Park Virus (Dsus) KU754525 Bat guano associated nodavirus ADI48250 77|41 Alphanodavirus ADF97523 Alphanodavirus 47|17 Santeuil nodavirus ADW54431 97|99 Orsay virus ADW54428 91|41 Le Blanc nodavirus AFU90716 TSA Panicum hallii JR063831 TSA Aleochara curtula GATW01051453 54|21 Plasmopara halstedii virus A ADK55578 D: Birnaviruses Paralichthys olivaceus birnavirus ABW07822 75|70 Marine birnavirus AAN04574 95|95 Yellowtail ascites virus AAN04570 55|51 Infectious pancreatic necrosis virus ACD14075 Aquabirnavirus 0.5 AA subs 87|68 Victorian trout aquabirnavirus ALF95259 57|60 Tasmanian aquabirnavirus ALF95254 Infectious bursal disease virus ABG91145 Avibirnavirus 100|99 Blotched snakehead virus CAD30691 Blosnavirus Rotifer birnavirus strain Palavas CAX33878 94|83 Tellina virus 1 CAI74982 Espirito Santo virus AEW87520 88|98 Culex Y virus AFR34027 70|64 98|100 Mosquitoe x virus AFU34334 96|98 Culicine-associated Z virus AGW51781 Eridge Virus (Dimm) KU754528 93|88 Entomo- Drosophila X Virus AF196645 birnavirus TSA Eurytemora affinis GBGO01030594 TSA Panonychus ulmi GCAC01001531 Drosophila melanogaster birnavirus ACU32792 E: Rhabdoviruses TSA Oropsylla silantiewi GAWY01012238 93|87 Sanxia Water Strider Virus 5 AJG39120 0.5 AA subs 71|50 Jingshan Fly Virus 2 AJG39108 20|43 Shuangao Bedbug Virus 2 AJG39134 89|87 Drosophila busckii Rhabdovirus KR822813 Lye Green Virus (Dobs) KU754522 Wuhan Mosquito Virus 9 AJG39218 Spodoptera frugiperda rhabdovirus KF947078 56|33 TSA Triodia sylvina GAVB01022634 96|96 Wuhan Ant Virus AJG39155 14|45 TSA Frankliniella occidentalis GAXD01021508 Taastrup virus AY423355 42|37 Di. longicaudata rhabdovirus ALU09129 Unclassified 95|75 Withyham Virus (Dobs) KU754523 Rhabdoviridae Drosophila subobscura Rhabdovirus KR822817 Drosophila sturtvanti Rhabdovirus KR822823 52|63 Shayang Fly Virus 3 AJG39131 Wuhan Fly Virus 3 AJG39162 TSA Musca domestica GBAB01012379 Wuhan House Fly Virus 2 AJG39173 Shuangao Insect Virus 6 AJG39136 99|98 Soybean cyst nematode virus AEF56733 Cherry Gardens Virus (Dsub) KU754524 97|75 Farmington virus YP 009091823 34|52 TSA Humulus lupulus LA363981 Tacheng Tick Virus 7 AJG39148 F: Reoviruses Soudat Virus (Sdef) KU754531 Torrey Pines virus (Dmel) KP714078 18|55 Bombyx mori cypovirus 1 AAK20302 100|99 Lutzomyia reovirus 2 AKP18622 0.5 AA subs 65|75 TSA Helicoverpa armigera GBDM01000458 75|69 TSA Helicoverpa assulta GBTA01063990/91 83|89 Heliothis armigera cypovirus 5 ABV04399 Cypovirus 76|83 Thaumetopoea pityocampa cypovirus 5 AJC97790 Aedes pseudoscutellaris reovirus AAZ94069 53|76 Fako virus AIW39871 Dinovernavirus Antheraea mylitta cypovirus 4 ADH10220 Dendrolimus punctatus cypovirus 22 AIY60598 Inachis io cypovirus 2 AHJ14791 Grape Cabernet Sauvignon reovirus AKB94056 Raspberry latent virus ADO27688 Rice ragged stunt virus AAC36456 Oryzavirus 95|96 Acinopterus angulatus reovirus AFA28393 Spissistilus festinus reovirus AEC32488 Colorado tick fever virus AF133428 Coltireovirus Eyach virus AAM18342 Cryphonectria parasitica mycoreovirus-1 BAS02068 Mycoreovirus Mycoreovirus 3 BAC98431 Bloomfield virus (Dmel) AKH40310 Drosophila reovirus AKP18621 92|68 Grange Virus (Dsub) 7|33 42|54 TSA Delia antiqua GAWI01008154 GAWI01008660 TSA Teleopsis dalmanni GBBP01003533/34 TSA Hypothenemus hampei GDLS01017018 Mal de Rio Cuarto virus AAO73182 98|100 rice black-streaked dwarf virus AEI59023 Fiji disease virus AAK40249 Fijivirus 21|28 Diaphorina citri reovirus BAH28860 93|92 Nilaparvata lugens reovirus BAA08542 Cimodo virus AHF20703 Operophtera brumata reovirus ABB17205 G: Picornavirales 0.5 AA subs Fisavirus 1 AIY32625 94|83 Pow Burn Virus (Dsub) KU754519 TSA Anopheles sinensis GAFE01004028 Unclassified Husavirus ALE31139 97|92 Picornavirales 99|96 Posavirus 1 AKJ83380 Posavirus 3 AKJ83381 Posavirus 2 AER30034 H: Basally-branching Flaviviruses 0.5 AA subs TSA Sitobion avenae aphid GAPL01011202 Macrosiphum euphorbiae virus 1 ALK01984 Shuangao lacewing virus 2 ALL52886 97|91 Shayang fly virus 4 ALL52888 84|41 Eriocheir sinensis Cholera-like Virus AKM16625 38|43 TSA Eriocheir sinensis KA661626 Unclassified Xinzhou spider virus 2 ALL52885 Flaviviridae Soybean cyst nematode virus 5 YP_009028573 73|44 Tacheng tick virus 8 ALL52879 Hermitage Virus (Dimm) KU754511 82|76 TSA Conwentzia psociformis GAYH01026052 Takaungu Virus KU754513 Gentian Kobu-sho-associated virus BAM78286 I: Unclassified viruses cf. Poleroviruses and Sobemoviruses 0.5 AA subs Grom Virus (Dobs) KU754506 93|96 Prestney Burn Virus (Dsub) KU754507 26|31 Motts Mill virus KP714076 Ixodes scapularis associated virus 1 KM048318 95|84 80|54 Ixodes scapularis associated virus 2 KM048319 TSA Exoneura robusta HP930702 99|100 94|81 TSA Osmia cornuta GAGH01013362 95|87 TSA Frieseomelitta varia HP890469 Unclassified, TSA Euprymna scolopes GBHH01001052 cf. Polerovirus TSA Lygus hesperus GBHO01033620 89|83 Sobemovirus, TSA Anthonomus grandis GABY01018235 TSA Xanthostigma xanthostigma GAUI01015619 TSA Nilaparvata lugens GAYF01147345 99|100 81|72 Humaita-Tubiacanga virus KR003801 99|100 TSA Caligus rogercresseyi GAZX01036530 Braid Burn Virus (Dsus) KU754508 La Tartoire Virus (Sdef) KU754509 J: Iflaviruses Thaumetopoea pityocampa iflavirus 1 AJC98140 30|28 Lymantria dispar iflavirus 1 AIF75200 0.5 AA subs 14|31 Bombyx mori iflavirus BAS18834 Antheraea pernyi iflavirus AHI87751 99|97 Heliconius erato iflavirus AHW98099 TSA Spodoptera exigua GARL01030594 Kakugo virus BAD06930 5|37 99|99 Deformed wing virus AHL46850 Iflavirus Varroa destructor virus-1 AAP51418 100|99 Formica exsecta virus 2 AHB62422 99|100 62|66 98|94 TSA Stigmatomma oregonense GAXR01027015 TSA Tetramorium bicarinatum GASM01001722 TSA Mischocyttarus flavitarsis GAXM01039151 Slow bee paralysis virus ABS84820 87|64 Graminella nigrifrons virus 1 AJT58559 Nilaparvata lugens honeydew virus 1 BAN19725 TSA Nilaparvata lugens GANM01013490 Laodelphax striatella honeydew virus 1 AHK05791 Laodelphax striatellus picorna-like virus 2 AIX94679 97|72 Nilaparvata lugens