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ArticleTitle Cadophora margaritata sp. nov., and other fungi associated with the longhorn beetles Anoplophora glabripennis and Saperda carcharias in Finland Article Sub-Title Article CopyRight Springer International Publishing AG, part of Springer Nature (This will be the copyright line in the final PDF) Journal Name Antonie van Leeuwenhoek Corresponding Author Family Name Linnakoski Particle Given Name Riikka Suffix Division Department of Forest Sciences Organization University of Helsinki Address Helsinki, Finland Division Organization Natural Resources Institute Finland (Luke) Address Helsinki, Finland Division Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI) Organization University of Pretoria Address Pretoria, South Africa Phone +358 29 532 2289 Fax Email [email protected] URL ORCID http://orcid.org/0000-0002-3294-8088 Author Family Name Kasanen Particle Given Name Risto Suffix Division Department of Forest Sciences Organization University of Helsinki Address Helsinki, Finland Phone Fax Email URL ORCID http://orcid.org/0000-0002-9948-2200 Author Family Name Lasarov Particle Given Name Ilmeini Suffix Division School of Forest Sciences Organization University of Eastern Finland Address Joensuu, Finland Phone Fax Email URL ORCID Author Family Name Marttinen Particle Given Name Tiia Suffix Division Department of Forest Sciences Organization University of Helsinki Address Helsinki, Finland Phone Fax Email URL ORCID Author Family Name Oghenekaro Particle Given Name Abbot O. Suffix Division Department of Forest Sciences Organization University of Helsinki Address Helsinki, Finland Division Department of Plant Biology and Biotechnology Organization University of Benin Address Benin City, Nigeria Phone Fax Email URL ORCID Author Family Name Sun Particle Given Name Hui Suffix Division Department of Forest Sciences Organization University of Helsinki Address Helsinki, Finland Phone Fax Email URL ORCID Author Family Name Asiegbu Particle Given Name Fred O. Suffix Division Department of Forest Sciences Organization University of Helsinki Address Helsinki, Finland Phone Fax Email URL ORCID http://orcid.org/0000-0003-0223-7194 Author Family Name Wingfield Particle Given Name Michael J. Suffix Division Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI) Organization University of Pretoria Address Pretoria, South Africa Phone Fax Email URL ORCID http://orcid.org/0000-0001-9346-2009 Author Family Name Hantula Particle Given Name Jarkko Suffix Division Organization Natural Resources Institute Finland (Luke) Address Helsinki, Finland Phone Fax Email URL ORCID http://orcid.org/0000-0002-1016-0636 Author Family Name Heliövaara Particle Given Name Kari Suffix Division Department of Forest Sciences Organization University of Helsinki Address Helsinki, Finland Phone Fax Email URL ORCID http://orcid.org/0000-0003-0657-8885 Received 25 January 2018 Schedule Revised Accepted 7 June 2018 Abstract Symbiosis with microbes is crucial for survival and development of wood-inhabiting longhorn beetles (Coleoptera: Cerambycidae). Thus, knowledge of the endemic fungal associates of insects would facilitate risk assessment in cases where a new invasive pest occupies the same ecological niche. However, the diversity of fungi associated with insects remains poorly understood. The aim of this study was to investigate fungi associated with the native large poplar longhorn beetle (Saperda carcharias) and the recently introduced Asian longhorn beetle (Anoplophora glabripennis) infesting hardwood trees in Finland. We studied the cultivable fungal associates obtained from Populus tremula colonised by S. carcharias, and Betula pendula and Salix caprea infested by A. glabripennis, and compared these to the samples collected from intact wood material. This study detected a number of plant pathogenic and saprotrophic fungi, and species with known potential for enzymatic degradation of wood components. Phylogenetic analyses of the most commonly encountered fungi isolated from the longhorn beetles revealed an association with fungi residing in the Cadophora–Mollisia species complex. A commonly encountered fungus was Cadophora spadicis, a recently described fungus associated with wood-decay. In addition, a novel species of Cadophora, for which the name Cadophora margaritata sp. nov. is provided, was isolated from the colonised wood. Keywords (separated by '-') 1 New taxon - Alien invasive species - Cadophora sp. - Introduced species - Insect–fungus symbiosis - Longhorn beetles - Vectored pathogen Footnote Information Electronic supplementary material The online version of this article (https://doi.org/10.1007/ s10482-018-1112-y) contains supplementary material, which is available to authorized users. Antonie van Leeuwenhoek https://doi.org/10.1007/s10482-018-1112-y 1 ORIGINAL PAPER 2 Cadophora margaritata sp. nov., and other fungi associated 3 with the longhorn beetles Anoplophora glabripennis 4 and Saperda carcharias in Finland 5 Riikka Linnakoski . Risto Kasanen . Ilmeini Lasarov . Tiia Marttinen . 6 Abbot O. Oghenekaro . Hui Sun . Fred O. Asiegbu . Michael J. Wingfield . 7 Jarkko Hantula . Kari Helio¨vaara Author Proof 8 Received: 25 January 2018 / Accepted: 7 June 2018 9 Ó Springer International Publishing AG, part of Springer Nature 2018 10AQ1 Abstract Symbiosis with microbes is crucial for obtained fromPROOFPROOFPopulus tremula colonised by S. 23 11 survival and development of wood-inhabiting long- carcharias, and Betula pendula and Salix caprea 24 12 horn beetles (Coleoptera: Cerambycidae). Thus, infested by A. glabripennis, and compared these to the 25 13 knowledge of the endemic fungal associates of insects samples collected from intact wood material. This 26 14 would facilitate risk assessment in cases where a new study detected a number of plant pathogenic and 27 15 invasive pest occupies the same ecological niche. saprotrophic fungi, and species with known potential 28 16 However, the diversity of fungi associated with insects for enzymatic degradation of wood components. 29 17 remains poorly understood. The aim of this study was Phylogenetic analyses of the most commonly encoun- 30 18 to investigate fungi associated with the native large tered fungi isolated from the longhorn beetles revealed 31 19 poplar longhorn beetle (Saperda carcharias) and the an association with fungi residing in the Cadophora– 32 20 recently introduced Asian longhorn beetle (Ano- Mollisia species complex. A commonly encountered 33 21 plophora glabripennis) infesting hardwood trees in fungus was Cadophora spadicis, a recently described 34 22 Finland. We studied the cultivable fungal associates fungus associated