A PUBLICATION JUL/AUG 2020 I www.terpenesandtesting.com TERPENES HORTICULTURE CHEMISTRY & ANALYTICS Certified Stats 101 Reference Understanding Accuracy, Precision, Error, and Uncertainty MATERIALS FOR THE Cannabis Industry Who Makes Your Standards? Solving qPCR’s LIVE-DEAD Problem Welcoming the Grim Reefer The Nuances OF TESTING Cannabis Mastering the Matrix CULTIVATING WITH Cannabaceae Cannabis, Hops, and a Hazy, Happy Family Reunion Developing Better Products through Natural Chemistry Penta Manufacturing Company is Family Owned and Operated for Over 40 Years. Here is a partial listing of our Family of Antioxidant ingredients utilized in flavor & fragrance formulations. 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Call 949.288.5337, or email [email protected], for more information. think20labs.com | Product intended for use only by qualified patients ages 18 and up Contents 05 The Fall is Coming Publisher Stats 101: Understanding Accuracy, MACE Media Group 06 Precision, Error, and Uncertainty CEO ISO Accreditation and the Nuances of Testing Celeste Miranda 12 Cannabis Matrices and Formulations Editor-in-Chief Jason S. Lupoi, Ph.D. Certified Reference Materials for the Cannabis Authors: 14 Industry: Solving the Production Puzzle Jason S. Lupoi, Ph.D. Cultivating with Cannabaceae: Patricia Atkins Alisia Ratliff 18 Cannabis and Hops Roxanne Franckowski Solving qPCR’s Bryant Jones Joshua Havill 22 Live-Dead Problem Ben Amirault Tips to Maximize Cultivation Yields in Robbie Batts Peter Saavedra 26 Your Commercial Grow Room Dr. Robert Bruck Terpenes Make Designer 30 Everything Better Marko Nedeljkovic Advertising: The Word According to Markus Julian Azevedo 32 Bradford Burgess Commodities Management Elite Genetics & Inputs Oso Vega Contact Us 600 CongressAvenue Logistics Austin, TX 78701 512-826-7781 [email protected] www.osovega.com TERPENES & TESTING 5 The Fall is Coming By Jason S. Lupoi, Ph.D. I once thought that 2020 would be the Year of Perfect Vision. I was a Schedule I drug on the federal level, devoid of proper medical wrong. Instead, humankind grapples with how to react, what to acknowledgement. Despite being discovered nearly 30 years ago, think, and where to find sense. Our world is anything but clear or doctors are just starting to learn about the endocannabinoid system. comforting. We’re facing a global pandemic and its aftershocks, Couple that notion to the fact that cannabis is the main plant that we a situation perhaps obscured or outright muddied by reports of know of that provides supplemental cannabinoids to help keep us misinformation and altered news. Ominous signs along roadsides balanced, and it’s no wonder there can be conspiratorial accusations. advise us to “stay home.” Many businesses had to close, at least Nevertheless, the movement of people recognizing the merit of temporarily, lengthening unemployment lines. cannabinoids and terpenes resembles a giant afghan that’s growing larger every day, snugly enveloping us. In Pittsburgh and many other cities across the country, these closures resulted in protests of armed business owners who didn’t Beyond the medicinal aspects, cannabis can provide infrastructure. agree with their Governor’s game plan. Wearing masks inside Wonderfully, despite cannabis still being that Schedule I drug, businesses seems to be a new norm. Large congregations of people industry businesses made the cut as ‘life-sustaining’ entities during aren’t yet allowed. And just when many businesses re-opened their the COVID-19 shutdown. With unemployment numbers soaring, doors as the virus supposedly subsided, protests erupted across the adopting cannabis legalization legislation will create jobs across United States over systemic racism and police brutality. As a global diverse disciplines, from constructing growhouses or designing tribe, Earthlings are recoiling, and it doesn’t feel like that’s about to compliant extraction facilities, to staffing and certifying analytical change. labs and ensuring there’s knowledgeable dispensary staff available to help educate the increasing throng of cannabis consumers. The emotional fallout of these events, such as fear, post-traumatic stress disorder, anxiety, depression, or paranoia, add to an already How many more states (and countries) need to free their constituents mentally-emaciated humankind. Perhaps on the back burner of all from the shackles of Reefer Madness before all the others recognize of these stories is the fact that 2020 is an election year. Our current that they, too, can make this work? That cannabis isn’t a plant that President has reported that the 2020 election will be the “scandal incites drug-crazed, reckless abandon? We’re smarter than that, of our times,” a foreboding nod to even more chaos yet to come, wouldn’t you say? perhaps capping off a conspiracy-riddled presidency. We can’t continue like this. So, when the fall comes, Iet’s add at least three states to the medical cannabis list and at least one state saying ‘enough’s enough – we’re I can’t predict what will happen come November, but I know that going rec.’ (Looking at you Pennsylvania.) And while we’re at it, let’s cannabis legalization is a mandatory issue. We’ve got enough work on our state lawmakers to allow reciprocity for those patients madness on our plates to digest these days that it’s critical to finally visiting from other medical cannabis states. This way, we don’t have put “Reefer Madness” in its grave through universal truths like logic, to risk federal punishment for taking the medicine that a real doctor common sense, and science. All one really need to do is open their recommended. eyes and ears. Cannabis does the rest. When the fall comes, let’s make the most of it, and press to There are few other available options that can assist our current accelerate the speed of change. While global harmony might seem global situation in divergent ways like cannabis. The plant, of far too utopic for the current chapter, we can help write (right) the course, provides medicine to millions of people, and yet, it is still script. 6 TERPENES & TESTING Stats 101: Understanding Accuracy, Precision, Error, and Uncertainty By Patricia Atkins, SPEX CertiPrep Analytical