Adipose Stem Cells from Buccal Fat Pad and Abdominal Adipose Tissue for Bone tissue Engineering Elisabet Farré Guasch Dipòsit Legal: B-25086-2011 ISBN: 978-90-5335-394-3 http://hdl.handle.net/10803/31987 ADVERTIMENT. La consulta d’aquesta tesi queda condicionada a l’acceptació de les següents condicions d'ús: La difusió d’aquesta tesi per mitjà del servei TDX (www.tesisenxarxa.net) ha estat autoritzada pels titulars dels drets de propietat intel·lectual únicament per a usos privats emmarcats en activitats d’investigació i docència. No s’autoritza la seva reproducció amb finalitats de lucre ni la seva difusió i posada a disposició des d’un lloc aliè al servei TDX. No s’autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant al resum de presentació de la tesi com als seus continguts. En la utilització o cita de parts de la tesi és obligat indicar el nom de la persona autora. ADVERTENCIA. 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On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reproduction with lucrative aims is not authorized neither its spreading and availability from a site foreign to the TDX service. Introducing its content in a window or frame foreign to the TDX service is not authorized (framing). This rights affect to the presentation summary of the thesis as well as to its contents. In the using or citation of parts of the thesis it’s obliged to indicate the name of the author. AUTORITZACIÓ DELS DIRECTORS DE TESI PER A LA SEVA PRESENTACIÓ La Dra. Núria Casals Farré, el Dr. Carles Martí Pagès i la Prof. Dra. Jenneke Klein-Nulend, com a co-directors de la tesi doctoral: “Adipose Stem Cells from Buccal Fat Pad and Abdominal Adipose Tissue for Bone tissue Engineering” realitzada a la Facultat d’Odontologia pel doctorant Sra. Elisabet Farré Guasch, autoritzen la presentació de l’esmentada tesi doctoral opció a Menció Europea, atès que reuneix les condicions necessàries per a la seva defensa. Sant Cugat, 09 de Decembre del 2010 ELS CO-DIRECTORS DE LA TESI Dra. Núria Casals Farré Dr. Carles Martí Pagès Prof. Dra. Jenneke Klein-Nulend The research presented in this thesis was carried out at: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitat Internacional de Catalunya (UIC), Barcelona, Spain. Department of Basic Sciences, Faculty of Medicine and Health Sciences, Universitat Internacional de Catalunya (UIC), Barcelona, Spain. Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA)- University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands. In kind cooperation with: The Flow Cytometry Research Core from Institut de Biotecnologia i Biomedicina UAB, Barcelona, Spain. Department of Orthopaedics, VU University Medical Center, Research Institute MOVE, Amsterdam, The Netherlands. Publication of this thesis was partially financially supported by: STEGA MOVE/Skeletal Tissue Engineering Group Amsterdam (STEGA), Amsterdam, The Netherlands. Institut Straumann AG, Basel, Switzerland. Roche Diagnostics Nederland BV, Almere, The Netherlands. ISBN/EAN: 978-90-5335-394-3 2011© Elisabet Farré Guasch, Barcelona, Spain. All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any mean, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the copyright owner. Printed by Ridderprint. Layout and cover design by E. Farré Guasch & N. Vermeulen. Universitat Internacional de Catalunya Oral & Maxillofacial Department, Faculty of Dentistry Basic Sciences Department, Faculty of Medicine and Health Sciences Adipose Stem Cells from Buccal Fat Pad and Abdominal Adipose Tissue for Bone Tissue Engineering PhD dissertation European Mention Elisabet Farré Guasch born in Barcelona Director: Dra. Núria Casals Farré Co-Director: Dr. Carles Martí Pagès Co-Director: Prof. Dra. Jenneke Klein-Nulend Barcelona, 2011 Contemplant el parpelleig de les estrelles, Trepanant la imponent volta de la nit Com s´enfila més encara l´esperit Fins a Vós, Senyor de tantes meravelles I et condolen els que acluquen les parpelles Al misteri fecundant de l´infinit Encallats al seu món físic… Tan petit Que no hi ha cap l´afany d´haver les coses belles Josep Farré i Gual To my family, with all my love “Those who have a 'why' to live, can bear any 'how'”. Adaptation of Viktor Frankl “Life would be less fun without fat” Acknowledgments AGRAÏMENTS I can not end this chapter without mentioning all the people who has contributed in some way or another to the configuration of this book. What starts has to end, and the ending is not less important than the start, although the ending remains more in the memories, a good end is always better than a good start. I remember my start, in 2005, doubting if to begin this path, although I had always clear where to start, in my home, at the University where I learnt and I became a professional, in the International University of Catalonia, Barcelona, where brought me my best memories and my best experiences decisive to consolidate my life. Although I was in that moment far away, if it can be nowadays considered far with the changes of distances thanks to the easiness of flying, I had clear that I had to begin with it here, and left everything there, the work, the patients, the friends, and a project of life, and came here to start this project. A start is always more difficult than a continuation, but I can tell that this start has taught me a lot, and I had the satisfaction to be able to decide where to lead the path. I was very lucky to meet Dr Giner, an excellent professional and person, and he opened the door to begin with it. Esme, you are not here now, but I hope you can read this, thank you for all your support and what you did while I was here. Afterwards I contact another excellent and professional person, Dr Martí, although in that moment it was a bit difficult to start contact with him personally, because his spirit of service and professionality lead him to work at that moment in the southeast of Africa, I finally met him in person and he accepted to guide my work. His curiosity and research interest lead him to work in that moment with stem cells, and that let me be able to start the idea of project I had, which was very new here in that moment, and that was to use stem cells for regeneration of maxillars. Afterwards, and when I was still in The Netherlands, I contacted Dr Clotet, a bright mind and intriging professor, who I was lucky to have met in my first years of study at the University and one of the people who encouraged my science interest, what I guess has always been there, but like what does the wind to a fire, it can grow or extinguish. Unfortunately, he was working with bacterias, and that was incompatible with my project, specially wished to avoid as much as possible working with cells. That brought me to meet Dra Casals, another brilliant professor and researcher, and a very social person, who has helped me a lot to improve and develop some other skills. Although in that moment I was a new person here in the lab, joining to the few team we were 6 Elisabet Farré Guasch Acknowledgments in the beginnings, and although I was a dentist, not a biology or biochemistry person, she decided to accept me in her team and guide me, and not less important, sustain also my research, morally and financially, and because all this, I am and I will always be thankful to her for being so brave. Afterwards I joined to the lab team, very tiny in that moment, as I said, and I met Ester, Patri, Anna and Diana. Ester, you are a great friend and collegue, and thank you a lot to motivate so much and be so hard with me, especially in the beginning, to make me learn a lot. You are really a practical person, and with a great interest to teach people. Thank you. Patri, I have also learnt a lot from you, and with you I had a lot of enjoyment with the performing of the first experiments, and you are always willing to give a hand to the next. That is really appreciated in the lab. Anna, you are not here now, but I also had a lot of fun with you, at the lunch in the bar, and I still remember your fibroblasts, which do look like stem cells, as you said. Adriana, you are far away now, in Colombia, but I will also remember you as a kind person, who always gave nice advice and you were the first to present the thesis in the department, and your funny comments about some Spanish words that were strange for you to use.