SEQUENTIAL CULTURE MEDIA SYSTEM Contents Gamete solutions ................................................................................ 7 Fertilization solutions ........................................................................15 Cleavage solutions ............................................................................21 Blastocyst solutions...........................................................................25 Complete list of order numbers ....................................................30 Nurture gametes and embryos with the nutrients they need. Our culture system mimics the delicate and dynamic environment of the female reproductive tract by providing nutrients to meet the shifting metabolic requirements of gametes and embryos. SOLUTIONS GAMETE Gamete collection and preparation might be the most stressful process in IVF, and the environmental disruption that occurs can significantly impair gamete viability. These gamete solutions meet the metabolic requirements of the oocyte and help to maintain oocyte homeostasis so that subsequent steps in IVF can succeed. 7 GAMETE FERTILIZATION CLEAVAGE BLASTOCYST SOLUTIONS SOLUTIONS SOLUTIONS SOLUTIONS 8 SYDNEY IVF GAMETE BUFFER Sydney IVF Gamete Buffer is used to physically wash and CONSTITUENTS store gametes in preparation for the fertilization step in the Calcium lactate L-proline IVF process. D-glucose L-serine • Designed to be used in atmospheric air conditions, not Gentamicin Magnesium sulphate Glutamine, stabilized Potassium chloride SOLUTIONS GAMETE an enriched CO2 environment Glycine Potassium phosphate • HEPES buffered HEPES Purified water • Designed to prepare oocytes and maintain a stable envi- Human serum albumin (HSA)* Sodium bicarbonate ronment during washing of cumulus-enclosed oocytes. L-alanine Sodium chloride • Designed to prepare sperm for swim-up, density L-asparagine Sodium pyruvate gradient, and diluent procedures L-aspartic acid Taurine L-glutamic acid • Can be used during ICSI SOLUTIONS FERTILIZATION USAGE RELEASE SPECIFICATIONS Sydney IVF Gamete Buffer is designed for gamete washing, pH (in air): 7.3-7.5 swim-up procedures, and use as a diluent in density Osmolality: 285-295 mOsm/kg gradient and sperm-thawing procedures. It can also be used 2-cell MEA: ≥ 80% of control expanded for ICSI when a HEPES-buffered environment is required. to blastocysts at 72 hours After ovum aspiration, Sydney IVF Gamete Buffer is used to Endotoxins: < 0.4 EU/mL wash the cumulus-oocyte complex before it is transferred Shelf life: 20 weeks from date of manufacture into Sydney IVF Fertilization Medium. The nonessential Sterile: Aseptically filtered SOLUTIONS CLEAVAGE amino acids prevent the depletion of the oocyte’s internal HSA assay: 5.00–12.50 mg/mL pool of amino acids. HSSA: ≥ 90% of control at 24 hours Order Reference Volume Number Part Number mL G46320 K-SIGB-20-AA 20 G46318 K-SIGB-50-AA 50 G46317 K-SIGB-100-AA 100 SOLUTIONS BLASTOCYST RELATED PRODUCTS Sydney IVF Follicle Flush Buffer Sydney IVF Spermient® Sydney IVF Sperm Gradient Kit Cook® Flexipet® Pipettes We strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure. *We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donations and plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinal blood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have been linked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure. 9 SYDNEY IVF SPERM MEDIUM Sydney IVF Sperm Medium is intended for use during in CONSTITUENTS vitro fertilization procedures to process sperm. Calcium lactate Potassium chloride • Used for sperm preparation and storage D-glucose Potassium phosphate Gentamicin Purified water • Recommended for swim-up and sperm washing in SOLUTIONS GAMETE Glutamine, stabilized Sodium bicarbonate 6% CO 2 Glycine Sodium chloride USAGE Human serum albumin (HSA)* Sodium pyruvate Sydney IVF Sperm Medium is designed for use with the Magnesium sulphate Taurine swim-up technique to separate motile populations of sperm. Intrauterine insemination can be performed in this medium, RELEASE SPECIFICATIONS or sperm can be resuspended in Sydney IVF Fertilization pH (in air): 7.5-7.8 Medium prior to the insemination of oocytes in vitro. The Osmolality: 285-295 mOsm/kg SOLUTIONS FERTILIZATION product should be equilibrated in 6% CO2 before use. 2-cell MEA: ≥ 80% of control expanded to blastocysts at 72 hours Order Reference Volume Number Part Number mL Endotoxins: < 0.4 EU/mL G46316 K-SISM-20-AA 20 Shelf life: 20 weeks from date of manufacture G46315 K-SISM-50-AA 50 Sterile: Aseptically filtered G46314 K-SISM-100-AA 100 HSA assay: 5.00–12.50 mg/mL RELATED PRODUCTS SOLUTIONS CLEAVAGE Sydney IVF Gamete Buffer Sydney IVF Sperm Gradient Kit Sydney IVF Fertilization Medium IUI catheters SOLUTIONS BLASTOCYST We strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure. *We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donations and plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinal blood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have been linked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure. 10 SYDNEY IVF SPERM GRADIENT KIT Sydney IVF Sperm Gradient is intended for use during in CONSTITUENTS vitro fertilization procedures to separate motile sperm from Calcium lactate Potassium phosphate seminal plasma. D-glucose Purified water • Sold as a convenient two-part kit for separating motile Gentamicin Silane-coated silica sperm Glutamine, stabilized particles SOLUTIONS GAMETE Glycine Sodium bicarbonate • Contains silane-coated silica HEPES Sodium chloride • Includes one vial of 40% solution and one vial of 80% Human serum albumin (HSA)* Sodium pyruvate solution Magnesium sulphate Taurine Potassium chloride USAGE Sydney IVF Sperm Gradient was designed for use in sperm RELEASE SPECIFICATIONS preparation via density-gradient separation. The gradients pH (in air): 7.3-7.5 SOLUTIONS FERTILIZATION are prepared by adding 1.5 mL of 40% solution underlaid Osmolality: 285-295 mOsm/kg with 1.5 mL of 80% solution in a conical-bottom test tube. 2-cell MEA: ≥ 80% of control expanded The liquefied semen is then overlaid onto the gradients. The to blastocysts at 72 hours test tube is centrifuged, and the resultant pellet is aspirated Endotoxins: < 0.4 EU/mL and washed in Sydney IVF Gamete Buffer. The final pellet is Shelf life: 20 weeks from date of manufacture resuspended in either Sydney IVF Sperm Medium or Sydney Sterile: Aseptically filtered IVF Fertilization Medium. HSA assay: 5.00–12.50 mg/mL Order Reference Volume Number Part Number mL SOLUTIONS CLEAVAGE G46313 K-SISG-20-AA 20 (x2) G46312 K-SISG-50-AA 50 (x2) RELATED PRODUCTS Sydney IVF Gamete Buffer Sydney IVF Sperm Medium Sydney IVF Fertilization Medium SOLUTIONS BLASTOCYST We strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure. *We take standard measures to prevent our medicinal human-blood and plasma products from causing infections. These steps include screening individual donations and plasma pools for specific markers of infection and inactivating and removing viruses during manufacture. Despite these safeguards, a possibility exists that medicinal blood or plasma products will transmit infective agents, including unknown or emerging agents or other pathogens. No reports of proven virus transmission have been linked to albumin that was manufactured by established processes to European Pharmacopoeia specifications. We strongly recommend that you record the patient name and lot number every time you use Cook Medical IVF media in a clinical procedure. 11 SYDNEY IVF SPERMIENT® Sydney IVF Spermient is intended for use during in vitro CONSTITUENTS fertilization procedures to separate motile sperm from Calcium lactate Potassium chloride seminal plasma. D-glucose Potassium phosphate • Sold at a 100% concentration of silane-coated-silica– Gentamicin Purified water SOLUTIONS GAMETE based stock solution to enable customization of sperm Glutamine, stabilized Silane-coated silica density separation Glycine particles HEPES Sodium bicarbonate • Can be diluted with Sydney IVF Gamete Buffer to any Human serum albumin Sodium chloride required concentration (HSA)** Sodium pyruvate USAGE Magnesium sulphate Taurine Sydney IVF Spermient is a 100% stock solution that should be diluted with Sydney IVF Gamete Buffer to the density RELEASE SPECIFICATIONS SOLUTIONS FERTILIZATION required for use (for example,