Catalogue 2017 Ion Channels and GPCRs February 2017 Glossary ION CHANNEL .................................................................................... 3 PROTEINASE ACTIVATED ...................................................... 33 GABA ................................................................................... 3 PURINERGIC ...................................................................... 33 3 GLYCINE ............................................................................... RELAXIN ......................................................................... 34 4 POTASSIUM CHANNEL ............................................................. RETINOID ACIDE INDUCED GENE ........................................... 35 PURINOCEPTOR ...................................................................... 5 RHODOPSIN ...................................................................... 35 SEROTONIN ........................................................................... 6 SECRETIN ......................................................................... 35 ZINC ACTIVATED ION CHANNEL ................................................. 7 SEROTONIN ...................................................................... 35 GPCR .................................................................................................... 8 SOMATOSTATIN ................................................................. 37 ADENOSINE ........................................................................... 8 SPHINGOSINE 1-PHOSPHATE ................................................ 37 ADRENERGIC ......................................................................... 8 TACHYKININ ...................................................................... 38 ANGIOTENSIN ........................................................................ 9 THYROTROPIN .................................................................... 39 BOMBESIN ............................................................................ 10 TRACE AMINE .................................................................... 39 BRADYKININ .......................................................................... 10 VASOPRESSIN .................................................................... 39 CALCITONIN .......................................................................... 10 VIP ................................................................................. 40 CALCIUM SENSING .................................................................. 10 CANNABINOID ....................................................................... 11 CHEMOKINE .......................................................................... 11 CHOLECYSTOKININ .................................................................. 13 COMPLEMENT PEPTIDE ............................................................ 14 CORTICOTROPIN -RELEASING FACTOR .......................................... 14 DOPAMINE ............................................................................ 14 ENDOTHELIN.......................................................................... 15 ESTROGEN ............................................................................ 15 FOLLICLE STIMULATING HORMONE ............................................. 16 FORMYLPEPTIDE ..................................................................... 16 FREE FATTY ACID R .................................................................. 16 FRIZZLED ............................................................................... 17 GALANIN .............................................................................. 17 GASTRIN /CHOLECYSTOKININ ..................................................... 17 GLUCAGON ........................................................................... 17 GLUCAGON LIKE PEPTIDE .......................................................... 18 GLUCOSE-DEPENDENT INSULINOTROPIC ...................................... 18 GONADOTROPIN -RELEASING HORMONE ...................................... 18 HISTAMINE ............................................................................ 18 HYDROXYCARBOXYLIC ACID ....................................................... 19 LEUKOTRIENE ......................................................................... 19 LUTROPIN -CHORIOGONADOTROPIC HORMONE ............................. 19 LYSOPHOSPHATIDIC ACID .......................................................... 19 MELANIN -CONCENTRATING HORMONE ....................................... 20 MELANOCORTIN ..................................................................... 20 MELATONIN .......................................................................... 21 METABOTROPIC GLUTAMATE .................................................... 21 METASTIN ............................................................................. 22 MUSCARINIC ACETYLCHOLINE .................................................... 22 NEUROMEDIN U..................................................................... 23 NEUROPEPTIDE Y.................................................................... 23 N-FORMYL PEPTIDE ................................................................ 24 OLFACTORY ........................................................................... 24 OPIOID ................................................................................. 24 OPSIN .................................................................................. 25 OREXIN ................................................................................ 25 ORPHAN ............................................................................... 25 PROKINETICIN ........................................................................ 32 PROLACTIN -RELEASING PEPTIDE ................................................. 32 PROSTANOID ......................................................................... 32 Edito Now that the purifica on of membrane receptors has become a more realis c objec ve due to the progresses made the recent years in new genera on of detergents and addi ves, a robust and compe ve offer was s missing for highly expressing stable cell lines. Theranyx is proud to offer the first catalogue dedicated to receptors over expressing cell lines perfectly suited for structural and biophysical characterisa on applica ons. Our ambi on is to offer you the highest standard of expression level for a broad range of func onal receptors in form of stable cell lines and membrane prepara ons suitable for purifica on and related applica ons, including ligand binding assays, SPR, an ody selec on, RMN and Xray crystallography. CHO/HEK tetracycline inducible cell lines All our cell line references are tetracycline inducible. This offers many advantages over classical CMV promoter based cell line including lower toxicity induced by the receptor expression during the cell growth phase, a more stable expression over the me and a higher level of expression for most of our products (over million receptors per cell). Inducible cell lines are also well suited for 13C 15N labelling on a short period of me with a limited background labelling. Our receptor constructs are carrying a double twin-strep-tag at the N-Terminus and a 10 his dine tag at the C-Terminus thus permi ng affinity purifica on on streptac and/or nickel/cobalt column but also immobilisa on of the receptors on beads or coated surface. Two TEV cleavage sites inserted between the tags and the receptor for tags removal. Moreover, customized constructs cell lines with alterna e tags can be developed at very a rac ve prices within 6 weeks for any reference of our catalogue. Cell line for receptors not available in our catalogue offer can be developed for the same price within 2 months. Scale-up : Our CHO and HEK cell lines are well suited for scale-up expression in roller bo les allowing to collect 1 to 2 g of cell pellet/bo le (detailed protocols are delivered with the cell lines). Bulk orders can be places for cell pellet or membrane prepara ons at compe ve prices. Consignment and prices Our stable cell lines are delivered either in frozen vials (worldwide) or in culture flasks at room temperature (Europe only). In both cases we also supply 100 ml of serum which has been validated for our cell lines, we leave to our customers to validate various serum lots from their usual suppliers. The prices of our cell line are fixed according to the following criteria: - 10 000 € for the cell lines produced upon order (delivery me 6 to 8 weeks) - 8 000 € for the cell lines immediately available. Notice! All our stable cell lines are characterised by radio ligand binding as soon as a radio ligand is commercially available. This policy has the following consequences: - You will pay a non characterised cell line for a lower price than the characterised ones. - If the characterisa on by radio ligand does not lead to significant specific binding (more than 1000 specific DPM for 10 µg of membrane prepara on issued from the cell line), the cell line will be reimbursed. -1- Applications Radio binding Western-blot Membrane preparation/solubilization/purification Phage display selection (antibody or peptide) Functional assay Flow cytometry Immuno staining‏ NMR SPR Receptor enriched membrane preparations Our receptor enriched membrane preparations are produced from highly expressing cell lines and thus guaranty a very good specific binding ratio. All our membrane preparations are delivered in consignments of 1000 units. For all membrane preparations for