Catalog # Aliquot Size P25-911-05 3 x 5 nmol P25-911-20 3 x 20 nmol P25-911-50 3 x 50 nmol PIK3C2G siRNA Set I siRNA duplexes targeted against three exon regions Catalog # P25-911 Lot # Z2073-49 Specificity Formulation PIK3C2G siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PIK3C2G expression. shipped at room temperature. Product Description Reconstitution Protocol PIK3C2G siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human PIK3C2G collect lyophilized siRNA at the bottom of the tube. mRNA expression. Each siRNA is 19-25 bases in length. The Resuspend the siRNA in 50 µl of DEPC-treated water gene accession number is BC130277. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot MGC163149; PI3K-C2GAMMA 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or PIK3C2A, Active P23-11G below -20oC. After resuspension, siRNA stock solutions ≥2 PIK3C2G, Active P25-11G µM can undergo up to 50 freeze-thaw cycles without PIK3C3 (Vps34), Active P26-10G significant degradation. For long-term storage, it is PIK3C2B Protein P24-31BG recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background PIK3C2G belongs to the phosphoinositide 3-kinase (PI3K) PIK3C2G siRNA Set I family which plays main roles in signaling pathways and siRNA duplexes targeted against three exon regions involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein Catalog # P25-911 trafficking (1). PIK3C2G contains a lipid kinase catalytic Lot # Z2073-49 domain as well as a C-terminal C2 domain, a Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol characteristic of class II PI3-kinases that act as calcium- Format Lyophilized powder dependent phospholipid binding motifs which mediate Stability 1yr at -70oC from date of shipment translocation of proteins to membranes, and may also Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is mediate protein-protein interactions and the N-terminal recommended that the lyophilized region of PIK3C2G and the C-terminal C2 domain are and resuspended siRNAs are stored at required for enzymatic activity (2). or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles References without significant degradation. For 1. Rozycka, M.et.al: cDNA cloning of a third human C2- long-term storage, it is recommended domain-containing class II phosphoinositide 3-kinase, that the siRNA is stored at -70oC. For most favorable performance, avoid PI3K-C2-gamma, and chromosomal assignment of repeated handling and multiple this gene (PIK3C2G) to 12p12. Genomics 54: 569-574, freeze/thaw cycles. 1998. 2. Misawa, H. et.al: Cloning and characterization of a novel class II phosphoinositide 3-kinase containing C2 domain. Biochem. Biophys. Res. Commun. 244: 531- 539, 1998. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .