TMEM184B Antibody Cat. No.: 5683 TMEM184B Antibody Immunohistochemistry of TMEM184B in human lung Immunofluorescence of TMEM184B in human lung tissue tissue with TMEM184B antibody at 5 μg/mL. with TMEM184B antibody at 20 μg/mL. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%) TMEM184B antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TMEM184B. IMMUNOGEN: The immunogen is located within the last 50 amino acids of TMEM184B. TESTED APPLICATIONS: ELISA, IF, IHC-P, WB September 23, 2021 1 https://www.prosci-inc.com/tmem184b-antibody-5683.html TMEM184B antibody can be used for detection of TMEM184B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. POSITIVE CONTROL: 1) Cat. No. 1462 - Rat Lung Tissue Lysate 2) Cat. No. 10-101 - Human Lung Tissue Slide Properties PURIFICATION: TMEM184B Antibody is affinity chromatography purified via peptide column. CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: TMEM184B Antibody is supplied in PBS containing 0.02% sodium azide. CONCENTRATION: 1 mg/mL TMEM184B antibody can be stored at 4˚C for three months and -20˚C, stable for up to STORAGE CONDITIONS: one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: TMEM184B TMEM184B Antibody: FM08, HS5O6A, C22orf5, PSEC0108, Transmembrane protein 184B, ALTERNATE NAMES: Putative MAPK-activating protein FM08 ACCESSION NO.: NP_036396 PROTEIN GI NO.: 63259329 GENE ID: 25829 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References September 23, 2021 2 https://www.prosci-inc.com/tmem184b-antibody-5683.html TMEM184B Antibody: TMEM184B, also known as C22orf5, is a 407 amino acid multi-pass membrane protein and represents a novel gene in the activation of the MAPK signaling pathway. The gene encoding TMEM184B maps to human chromosome 22; mutations in BACKGROUND: several of the genes in chromosome 22 are involved in the development of autism, schizophrenia, Phelan-McDermid syndrome and Neurofibromatosis type 2, suggesting that TMEM184B may play a role in these syndromes. 1) Matsuda A, Suzuki Y, Honda G, et al. Large-scale identification and characterization of REFERENCES: human genes that activate NF-kappaB and MAPK signaling pathways. Oncogene2003; 22:3307-18. 2) Dunham I, Shimizu N, Roe BA, et al. The DNA sequence of human chromosome 2. Nature1999; 402:489-495. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 23, 2021 3 https://www.prosci-inc.com/tmem184b-antibody-5683.html.