PRODUCT DATA SHEET Bioworld Technology,Inc. DDX50 polyclonal antibody Catalog: BS71842 Host: Rabbit Reactivity: Human,Mouse,Rat BackGround: by affinity-chromatography using epitope-specific im- DEAD box proteins, characterized by the conserved motif munogen and the purity is > 95% (by SDS-PAGE). Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. Applications: They are implicated in a number of cellular processes in- WB 1:200 - 1:2000 volving alteration of RNA secondary structure such as IHC 1:50 - 1:200 translation initiation, nuclear and mitochondrial splicing, IF1:50 - 1:200 and ribosome and spliceosome assembly. Based on their Storage&Stability: distribution patterns, some members of this DEAD box Store at 4°C short term. Aliquot and store at -20°C long protein family are believed to be involved in embryogen- term. Avoid freeze-thaw cycles. esis, spermatogenesis, and cellular growth and division. Specificity: This gene encodes a DEAD box enzyme that may be in- DDX50 polyclonal antibody detects endogenous levels of volved in ribosomal RNA synthesis or processing. This DDX50 protein. gene and DDX21, also called RH-II/GuA, have similar DATA: genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined. Product: Western blot analysis of extracts of various cell lines, using DDX50 an- Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, tibody at 1:3000 dilution. 50% glycerol, pH7.2 Note: Molecular Weight: For research use only, not for use in diagnostic procedure. 105kDa Swiss-Prot: Q9BQ39 Purification&Purity: The antibody was affinity-purified from rabbit antiserum Bioworld Technology, Inc. Bioworld technology, co. Ltd. Add： 1660 South Highway 100, Suite 500 St. Louis Park, Add： No 9, weidi road Qixia District Nanjing, 210046, MN 55416,USA. P. R. China. Email: [email protected] Email: [email protected] Tel: 6123263284 Tel: 0086-025-68037686 Fax: 6122933841 Fax: 0086-025-68035151 .