Propagation of Ornamental Plants Vol. 20, № 4, 2020: 111-117 MICROPROPAGATION OF ASTROPHYTUM MYRIOSTIGMA LEM. THROUGH AREOLES ACTIVATION Kihwan Song1, Hyeonjeong Kang2, Doo Hwan Kim3, and Iyyakkannu Sivanesan3* 1Department of Bioindustry and Bioresource Engineering, Sejong University, 209 Neungdong-ro, Gwangjin-gu, 05006 Seoul, Republic of Korea 2 Babo Orchid Farm, Gyeonggi-do, 472-831 Namyangju-si, Republic of Korea 3Department of Bioresources and Food Science, Institute of Natural Science and Agriculture, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, 05029 Seoul, Republic of Korea *Fax: + 8224503310, *E-mail: [email protected] REFERENCES Castro-Gallo I. A., Meza-Rangel E., Pérez-Reyes M. E., Pérez-Molphe-Balch E. (2002). In vitro propagation of 10 Mexican cacti species. Scientiae Naturae, 4: 5-24 (in Spanish). Clayton P. W., Hubstenberger J. F., Phillips G. C. (1990). Micropropagation of members of the Cactaceae Subtribe Cactinae. Journal of the American Society of Horticultural Science, 115: 337-343. Das A. B. (2008). Assessment of genetic diversity and phylogenetic analysis of ‘Star Cactus’ (Astrophytum) through chromosome and RAPD markers. Cytologia, 73: 179-88. Erwin J., Altman K., Esqueda F. (2017). Temperature impacts cactus and succulent development rate. HortTechnology, 27: 65-68. García-Rubio O., Malda-Barrera G. (2010). Micropropagation and reintroduction of the endemic Mammillaria mathildae (Cac- taceae) to its natural habitat. HortScience, 45: 934-938. Giusti P., Vitti D., Fiocchetti F., Colla G., Saccardo F., Tucci M. (2002). In vitro propagation of three endangered cactus spe- cies. Scientia Horticulturae, 95: 319-332. Infante R. (1992). In vitro axillary shoot proliferation and somatic embryogenesis of yellow pitaya Mediocactus coccineus (Slam-Dy- ck). Plant Cell, Tissue and Organ Culture, 31: 155-159. Kim D. H., Enkhtaivan G., Saini R. K., Keum Y-S., Kang K. W., Sivanesan I. (2019). Production of bioactive compounds in cla- dode culture of Turbinicarpus valdezianus (H. Moeller) Glass & R. C. Foster. Industrial Crops & Products, 138: Article 111491. Lázaro-Castellanos J.O., Mata-Rosas M., González D., Arias S., Reverchon F. (2018). In vitro propagation of endangered Mammillaria genus (Cactaceae) species and genetic stability assessment using SSR markers. In Vitro Cellular & Developmental Biology - Plant, 54: 518-529. Lema-Rumińska J., Kulus D. (2014). Micropropagation of cacti: a review. Haseltonia, 19: 46-63. Machado M. F. P. S., Prioli A. J. (1996). Micropropagation of Cereus peruvianus Mill. (Cactaceae) by areole activation. In Vitro Cellular & Developmental Biology-Plant, 32: 199-203. Martínez-Vázquez O., Rubluo A. (1989). In vitro mass propagation of the near-extinct Mammillaria san-angelensis Sánchez- Mejorada. Journal of Horticultural Science, 64: 99-105. Mohamed-Yasseen Y. (2002). Micropropagation of pitaya (Hylocereus undatus Britton et rose). In Vitro Cellular & Developmental Biology-Plant, 38: 427-429. Murashige T., Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plan- tarum, 15: 473-497. Papafotiou M., Balotis G., Louka P., Chronopoulos J. (2001). In vitro plant regeneration of Mammillaria elongata normal and cristate forms. Plant Cell, Tissue and Organ Culture, 65: 163-167. Pérez-Molphe-Balch E., Pérez Reyes M. E., Villalobos Amador E., Meza Rangel E., Morones Ruiz L. R., Lizalde Viramon- tes H. J. (1998). Micropropagation of 21 species of Mexican cacti by axillary proliferation. In Vitro Cellular & Developmental Biology - Plant, 34: 131-135. Pérez-Molphe-Balch E., Santos-Díaz M., Ramírez-Malagón R., Ochoa-Alejo N. (2015). Tissue culture of ornamental cacti. Scientia Agricola, 72: 540-561. Ramirez-Malagon R., Aguilar-Ramirez I., Borodanenko A., Perez-Moreno L., Barrera-Guerra J., Nuñez-Palenius H. G., Ochoa-Alejo N. (2007). In vitro propagation of ten threatened species of Mammillaria (Cactaceae). In Vitro Cellular & Devel- opmental Biology-Plant, 43: 660-665. Rowley G. (2003). What is an areole? British Cactus & Succulent Journal, 21: 4-11. Vázquez-Lobo A., Morales G. A., Arias S., Golubov J., Hernández-Hernández T., Mandujano M. C. (2015). Phylogeny and biogeographic history of Astrophytum (Cactaceae). Systematic Botany, 40: 1022-1030. Vyscot J., Jára Z. (1984). Clonal propagation of cacti through axillary buds in vitro. Journal of Horticultural Sciences, 59: 449-452..