Figure S1 GFAPInd NB +bFGF/+EGF NB -bFGF/-EGF nestin GFAP DAPI (a) Figure S1 GFAPConst NB +bFGF+/+EGF NB -bFGF/-EGF nestin GFAP DAPI (b) Figure S2 +bFGF/+EGF (a) Figure S2 -bFGF/-EGF (b) Figure S3 #10 #1095 #1051 #1063 #1043 #1083 ~20 weeks GSCs GSC_IRs compare tumor-propagating capacity proliferation gene expression Supplemental Table S1. Expression patterns of nestin and GFAP in GSCs self-renewing in vitro. GSC line nestin (%) GFAP (%) #10 90 ± 1,9 26 ± 5,8 #1095 92 ± 5,4 1,45 ± 0,1 #1063 74,4 ± 27,9 3,14 ± 1,8 #1051 92 ± 1,3 < 1 #1043 96,7 ± 1,4 96,7 ± 1,4 #1080 99 ± 0,6 99 ± 0,6 #1083 64,5 ± 14,1 64,5 ± 14,1 G112-NB 99 ± 1,6 99 ± 1,6 Immunofluorescence staining of GSCs cultured under self-renewal promoting condition Supplemental Table S2. Gene expression analysis by Gene Ontology terms.