Upregulated Glucose Metabolism Correlates Inversely with CD8 T Cell

Upregulated Glucose Metabolism Correlates Inversely with CD8 T Cell

Author Manuscript Published OnlineFirst on May 20, 2016; DOI: 10.1158/0008-5472.CAN-15-3121 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. V rtyhrq typr rhiyv p ryhr vr ry vu 89' U pryy vsvy hv hq vhy v h pryy ph pvh 8u vvh C Prrvr Fhr G Qr @yrh G Ch qr Ehh Thhxh Wr vxh Errv Eh Ayrvt Pyvr Xq Ertv X! 8u vur C Xryx! Bh ru E Uuh " Trur H Uuv qi tu " ! Svt vyr ! Fr q" #$ %$ !& Avhpvhy ' & ( )*+,-./,/0 8 rqvt hu & 1 " 1234+ ( 56678 ) *9++04:34;/<4: & 1= > Avt r hq hiyr< 1 * / 0?: & :! Downloaded from cancerres.aacrjournals.org on September 30, 2021. © 2016 American Association for Cancer Research. Author Manuscript Published OnlineFirst on May 20, 2016; DOI: 10.1158/0008-5472.CAN-15-3121 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. 6i hp . & &> 1 > 1 @' > &> 1 ' 1 & 1 &A @ ' 1 ! 1 * #$0 B * 0 1 & 1 1 & & ' 1 C > #$& 1 1 &%( @#&' 1 & ' 4 Downloaded from cancerres.aacrjournals.org on September 30, 2021. © 2016 American Association for Cancer Research. Author Manuscript Published OnlineFirst on May 20, 2016; DOI: 10.1158/0008-5472.CAN-15-3121 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. D qpv & 1 $.!+ 28!-28!$ 1 *0 .& > 1 ! & ! 1 &> & 1 *0 & > &> 1 & &' 1 >& & 1 128!' D & 1 #$ 28!$*4:0 1 1 > &> > ! 1 ! &A E #$ B *+0 ' ' @2F! > B *@E 0 */0 . 3/G @2F! @E 1 ! 11 1 1 & > @2F! @E ' 1 #$ & & >> ! > 1 *60 *<0 :9 1 ! ! *3,0 & 1& ' 1 & #$ B' & 1 % . * %.0 1 ' 1 1 : Downloaded from cancerres.aacrjournals.org on September 30, 2021. © 2016 American Association for Cancer Research. Author Manuscript Published OnlineFirst on May 20, 2016; DOI: 10.1158/0008-5472.CAN-15-3121 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. ' > *C%E.0 *;0 E' > & ! ' ' 1 ! ' 1 1 1 &' ' C%E. 1 & 11 ' > '1 ' & 1 &' 1 ' ' >*0 1 #$ ' 1 1 1 1 1' & D @# 1 + Downloaded from cancerres.aacrjournals.org on September 30, 2021. © 2016 American Association for Cancer Research. Author Manuscript Published OnlineFirst on May 20, 2016; DOI: 10.1158/0008-5472.CAN-15-3121 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Hhr vhy hq Hruq WGCNA (' 1 1 > 1 1 ' %. 1(*40 D 1 !1 *:05 &A ' 1 ' > & *+0C%E.' ! 1C%E.>1 (*; /0 E' > ' 1 & 1 1 ' 1 % *--1 1-0 1 . 4 1 ' & 1 *% .0 1 ! > *--'''& 1-0 Survival analyses &' 1 ' D 1 2@ ' D 1 1 ' 1 1 ' 1! > * ( 0)! 2@ ' 1 &''1 1 ( >1 Immunohistochemistry ' 1 ! & *. 4 10 . 1 *F H 0 ' & 1 1 .& 1 8: 83 *$ 0 %( *8>0 / Downloaded from cancerres.aacrjournals.org on September 30, 2021. © 2016 American Association for Cancer Research. Author Manuscript Published OnlineFirst on May 20, 2016; DOI: 10.1158/0008-5472.CAN-15-3121 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. %$ ! @#! *.&0 #$ ' B 1 J . (/ J . ' *+9399;0. 1 ! #$ - 1!' ' C ' . 1 #$ ' . 1 ' '! #$ */0 Cell culture and reagents ! 1 /<"$-6 2 ' & . 14;!4;4 " ' (2#6+; ' ;G 4$!1 ;; -$ ;;)1-$ * 1!. 0 ' 1 Mycoplasma Retroviral transduction !18E. %$ !' &( !2( F! *0 ( ' 1 2 >1 1 ' ' 431- * 1! . 0 %$ ! ' ' B ' !%$ !* 2+,3?.&0 # !A1 1 ! #1%*N> #( $ 0 Analysis of cellular glycolysis 6 Downloaded from cancerres.aacrjournals.org on September 30, 2021. © 2016 American Association for Cancer Research. Author Manuscript Published OnlineFirst on May 20, 2016; DOI: 10.1158/0008-5472.CAN-15-3121 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. ' 1 " H,6 .D *E " . .0 !& 8 * 1!. 0 ' 4$!1 @<+ ' H,6 ! ' ! > *"8 " 0 +;+ -' ' 1 ' 1 *;/ 0 4!8%*+; 0' A Tumor therapy experiments /<"-6 1 6!3'> ' A & ' /;/ ! ' F! F!%$ !! > . : ' & ' 1 D ' ! <-6! 18E. 1 ' ' ' :!'> ' 1 *1' 4 . ' 1)@5 1 * & 22$:;-:;430 Statistical analysis 1 &' 1 ! C 1 ! > ) >!C * 1 41 0' 11 ' & ! ' M1; 1 1 1 ' 1 > ' 1 * 0 *1 0 1 1 1 ' ' > < Downloaded from cancerres.aacrjournals.org on September 30, 2021. © 2016 American Association for Cancer Research. Author Manuscript Published OnlineFirst on May 20, 2016; DOI: 10.1158/0008-5472.CAN-15-3121 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Sry The HNSC transcriptome is organized into consistent modules of coexpressed genes, which correspond to distinct biological processes 1 1 ' ' ' #$ & ' C%E. @E (E.! B *60 & & %.* 1 ' 0 D & @2F! *Q:+0 @2F! 1 *Q440 @2F! ' & 1 (E.! B @2F1 *60. ! 1 1 ' & ' &'1 +;;; 1 > & ' A C 4 1 1 ' & 1& 1 * 1.0. ' ' >& ' & ' & & --''' &- -6< DD4''+,1-.." #@,$7 )B#R Q; & ' 1 & 1 % 51 *%50 ' % * --1 1-0 . & 4 1 %51 1 > 6QR:Q 1 D R Q> R 3Q 1 ! R* & 0 . %51 & 4 3 Downloaded from cancerres.aacrjournals.org on September 30, 2021. © 2016 American Association for Cancer Research. Author Manuscript Published OnlineFirst on May 20, 2016; DOI: 10.1158/0008-5472.CAN-15-3121 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. ' >' & ' ' (E.! B ' ' @E *! ."!:;4 '''& >- ? --& >- -2& 2 A R Q:60*<305 +;;;1 ' >://<*33,G0' 1 ' > *,0 */ 1 1 Q 1 (E. & R0' 1 @E * & 0 ' 11 1 & @E > 1 / > & +3 (E.! B1 / & 1C21orf62&1 2 & @E *& 0' ' 1 *6 4; 40 &' ' %.!@E ' >* 1 "0 ' &' 1R & 1 1 ' > ' 11 @E ' 1 & * 1 !0 *:34 & 0 ' > & 1 *Q! R0 ' * 1 0 ' & 1 > ' - 1 & & ' 1 *4;0" & &' & EGFR MYC1 *40 ' HRAS-CASP8! *60 4 + * 1 80 1 , Downloaded from cancerres.aacrjournals.org on September 30, 2021. © 2016 American Association for Cancer Research. Author Manuscript Published OnlineFirst on May 20, 2016; DOI: 10.1158/0008-5472.CAN-15-3121 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. & ' ' ' ' > 1 1 ,* 10 6/ * 10 & & : , :B' @2F! >EGFR , D &*640 Coexpression modules are significantly associated with HNSC biomarkers 1 & ' D & 11*0*;0 ' 1 * 1 %0 1 1 ' >' @E & > 1 1 1 #$ B ' @2F! %(1 +*%5 > 0 ' 1 * +Q!;30 ' + 1 & ' '! > D 1 1 ! > !6"2 &' %( @2F! 1 *440 + 4*%5' 10' 1 ' EGFR1 * 4Q;66? +Q;660 ' @2F! * 4Q!;<3? +Q !;<:0 ' 1 ' #$ * Q;640 ' & 1 1 ' EGFR * Q!;//0 @2F! * Q;/:0 1 &' #$ %( ; Downloaded from cancerres.aacrjournals.org on September 30, 2021. © 2016 American Association for Cancer Research. Author Manuscript Published OnlineFirst on May 20, 2016; DOI: 10.1158/0008-5472.CAN-15-3121 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. 1 &'1 ' 1 ' & D 1 *4:0 2 1 1 .

View Full Text

Details

  • File Type
    pdf
  • Upload Time
    -
  • Content Languages
    English
  • Upload User
    Anonymous/Not logged-in
  • File Pages
    40 Page
  • File Size
    -

Download

Channel Download Status
Express Download Enable

Copyright

We respect the copyrights and intellectual property rights of all users. All uploaded documents are either original works of the uploader or authorized works of the rightful owners.

  • Not to be reproduced or distributed without explicit permission.
  • Not used for commercial purposes outside of approved use cases.
  • Not used to infringe on the rights of the original creators.
  • If you believe any content infringes your copyright, please contact us immediately.

Support

For help with questions, suggestions, or problems, please contact us