Authors: Maria Doyle Ron Caspi James I Macrae An online version of this diagram is available at BioCyc.org. Biosynthetic pathways are positioned in the left of the cytoplasm, degradative pathways on the right, and reactions not assigned to any pathway are in the far right of the cytoplasm. Transporters and membrane proteins are shown on the membrane. Eleanor C Saunders Periplasmic (where appropriate) and extracellular reactions and proteins may also be shown. Pathways are colored according to their cellular function. LeishCyc: Leishmania major strain Friedlin Cellular Overview Connections between pathways are omitted for legibility. Malcolm J Mcconville D-fructose D-fructose D-fructose hypoxanthine inosine 1-alkyl-2-acyl-phosphatidylcholine D-galactose D-galactose D-fructose D-galactose xanthine spermidine guanosine a phosphatidylcholine myo-inositol D-mannose D-mannose D-mannose D-mannose adenine arg arg putrescine D-glucose D-glucose D-glucose D-glucose guanine xanthosine myo-inositol/ ABC polyamine glucose glucose hexose glucose nucleobase proton transporter transporter transporter transporter transporter transporter transporter AAP3 AAP3 NT2 symporter (ABCG4) (POT1) (GT3) (GT1) (GT4) (GT2) (NT3) (MIT) 1-alkyl-2-acyl-phosphatidylcholine inosine arg arg a phosphatidylcholine spermidine D-fructose D-fructose D-fructose D-fructose hypoxanthine guanosine putrescine myo-inositol D-galactose D-galactose D-mannose D-galactose xanthine xanthosine D-mannose D-mannose D-glucose D-mannose adenine D-glucose D-glucose D-glucose guanine Fatty Acid and superpathway of sterol biosynthesis Lipid Degradation a [BCAA a [protein] N- Aminoacyl-tRNA Charging tRNA charging pathway fatty acid β-oxidation a purine carbonic acid 4,4-dimethyl-5-α-cholesta- Aldehyde Degradation dehydrogenase terminal-formyl- 4,4-dimethylzymosterol 4-methyl-2- 6 ribonucleoside leu 8,14,24-trien-3-β-ol E2 protein] N L-methionine phe a tRNA Phe coenzyme A oxopentanoate -lipoyl-L-lysine C-14 sterol glyoxalase pathway carbonic asp asp trp Trp lys Lys branched-chain tRNA a tRNA a tRNA reductase, peptide anhydrase- amino acid inosine- putative: G3IU-2308 long-chain-fatty- dihydroxyacetone D-glyceraldehyde- 2-oxoisovalerate deformylase, like protein: Ile Ala adenosine- ile a tRNA phenylalanyl- a tRNA ala aminotransferase, 4-α-methylzymosterol phosphate 3-phosphate dehydrogenase putative acid-coA ligase an acyl-CoA guanosine- G3IU-3847 vacuolar thr Thr asn Asn tRNA synthetase glt glu val Val his His aspartyl-tRNA gly Gly tryptophanyl- arg Arg met Met gln Gln lysyl-tRNA tyr Tyr leu Leu cys Cys ser ser putative: G3IU-3952 4,4-dimethylzymosterol beta subunit, (EMBL:AY353252) a tRNA tRNA a tRNA a tRNA a tRNA a tRNA a tRNA a tRNA a tRNA a tRNA a tRNA a tRNA a tRNA protein,putative: carbonic ATP alpha chain, synthetase, tRNA synthetase, synthetase, mitochondrial : G3IU-8117 nucleoside 4-methyl-2-oxopentanoate G3IU-3775 + + isoleucyl-tRNA putative: G3IU-2460 putative: G3IU-3237 putative: G3IU-3574 alanyl-tRNA putative: G3IU-6586 pro a tRNA Pro hydrolase, anhydrase H synthase H [+ 8 isozymes] precursor, polypeptide threonyl-tRNA synthetase, asparaginyl- phenylalanyl- glutamyl-tRNA valyl-tRNA histidyl-tRNA aspartyl-trna glycyl tRNA tryptophanyl- arginyl-tRNA synthetase, methionyl-tRNA glutaminyl-tRNA lysyl-tRNA tyrosyl-tRNA leucyl-tRNA cysteinyl-tRNA seryl-tRNA putative: family subunit D, methylglyoxal H + putative: deformylase- synthetase, putative: G3IU-149 trna synthetase, tRNA synthetase, synthetase, synthetase, synthetase, synthetase, synthetase, tRNA synthetase, synthetase, putative: G3IU-5205 synthetase, synthetase, synthetase, synthetase, synthetase, synthetase, synthetase, zymosterol G3IU-3293 protein, putative a 2,3,4-saturated G3IU-1271 like protein, putative: G3IU-1139 [+ 2 isozymes] putative: G3IU-1514 putative: G3IU-5831 putative: G3IU-2937 putative: G3IU-2949 putative: G3IU-3220 putative: G3IU-5472 putative: G3IU-332 putative: G3IU-5145 putative: G3IU-4026 [+ 2 isozymes] putative: G3IU-5495 putative: G3IU-6463 putative: G3IU-3271 putative: G3IU-6629 putative: G3IU-6830 putative: G3IU-7054 putative: G3IU-7207 a (3Z)-alk-3- putative: fatty acyl CoA 2-oxoisovalerate putative: G3IU-7886 vacuolar isovaleryl-CoA enoyl-CoA sterol 24-c- sterol 24-c- sterol 24-c- sterol 24-c- acyl-CoA dehydrogenase G3IU-2417 D-ribose ATP H + synthase H + Thr Ile Asn Phe Glu Val His asp Gly Trp Arg Ala Met Gln Lys Tyr Leu Cys ser Pro methyltransferase: methyltransferase: methyltransferase: methyltransferase: dehydrogenase, alpha subunit, a purine base an L-threonyl-[tRNA ] an L-isoleucyl-[tRNA ] an L-asparaginyl-[tRNA ] an L-phenylalanyl-[tRNA ] an L-glutamyl-[tRNA ] an L-valyl-[tRNA ] an L-histidyl-[tRNA ] an L-aspartyl-[tRNA ] a glycyl-[tRNA ] an L-tryptophanyl-[tRNA ] an L-arginyl-[tRNA ] an L-alanyl-[tRNA ] an L-methionyl-[elongator tRNA ] an L-glutaminyl-[tRNA ] an L-lysyl-[tRNA ] an L-tyrosyl-[tRNA ] an L-leucyl-[tRNA ] an L-cysteinyl-[tRNA ] an L-seryl-[tRNA ] an L-prolyl-[tRNA ] 3,2-trans- trypanothione SMT1.1 SMT1.2 SMT1.2 SMT1.2 putative: L-lactaldehyde putative: CO H O subunit c, isovaleryl-coA α β enoyl-CoA an N-terminal L- 2 2 5 -cholesta-7,24-dien-3 -ol hemithioacetal G3IU-5415 formate putative dehydrogenase, sterol 24-c- sterol 24-c- sterol 24-c- sterol 24-c- isomerase, G3IU-1008 methionyl-[protein] glyoxalase putative: G3IU-4068 methyltransferase: lathosterol methyltransferase: methyltransferase: methyltransferase: mitochondrial [+ 2 isozymes] P-type H+- acetyl-CoA i: GLO1 SMT1.2 oxidase-like SMT1.1 SMT1.1 SMT1.1 precursor- a [apo BCAA dehydrogenase H + ATPase, H + a 2-enoyl-CoA 6 Cofactor, Carrier, and Vitamin Biosynthesis 3-methylcrotonyl-CoA protein: G3IU-5023 like protein: S-lactoyl- E2 protein] N -S-[3- putative 24,24-dimethyl ergosta- G3IU-3579 trypanothione methylbutanoyl]dihydrolipoyl- fecosterol 7-dehydrodesmosterol 8,24(25)-ergostadienol 8,25(27)-ergostadienol methylcrotonoyl- 8,25(27)-dienol glyoxalase L-lysine folate metabolism [+ 2 isozymes] P-type H+- NAD biosynthesis molybdenum cofactor biosynthesis γ-glutamyl cycle pyridoxamine coa carboxylase II: G3IU- ubiquinone-9 biosynthesis acetoacetyl-CoA H + ATPase, H + II (from tryptophan) anabolism biotinylated 7057 a small subunit of a (2E)-2- CDP putative tetrahydrofolate all-trans- subunitprotein-like succinyl-CoA a 3-oxo acid a reduced N-acetyl- molybdopterin glutathione Secondary Metabolite Biosynthesis Other Biosynthesis enoyl-CoA xanthosine nonaprenyl pyridoxamine pyruvate protein: G3IU-2559 episterol Activation/Inactivation/Interconversion nitric oxide (R)-lactate tryparedoxin L-citrulline trp 4-hydroxybenzoate synthase diphosphate biosynthesis iron IAA biosynthesis I superpathway of (R,R)- 3-methylcrotonoyl- enoyl-CoA succinyl-coa:3- 2+ 2+ myo-inositol biosynthesis (R,R)-butanediol CoA carboxylase C-5 sterol homocysteine and cysteine interconversion hydratase/ a 2,3,4- D-lactate ribonucleoside- acetylornithine Fe transporter Fe butanediol biosynthesis ketoacid- N-formylkynurenine biosynthesis beta subunit, desaturase, arg isomerase- saturated (3R)-3- dehydrogenase- diphosphate deacetylase- inosine- (LIT1) a carboxy- coenzyme a formate- trp D-glucose 6-phosphate pyruvate putative: G3IU-7158 putative: G3IU-2821 like protein: hydroxyacyl-CoA like protein: reductase large like protein: adenosine- tetrahydrofolate 3-nonaprenyl-4- adenylated pyridoxal (R)-acetoin L-cystathionine transferase- guanosine- dihydrofolate myo-inositol- G3IU-3342 G3IU-3552 chain, putative: G3IU-2698 iron methionyl-trna ligase: FTL hydroxybenzoate [small subunit of glutathione broad specificity NADP-dependent 3-methylglutaconyl-CoA oxidoreductase- like protein: nucleoside reductase- 1-phosphate [+ 8 isozymes] d-lactate G3IU-3802 acetylornithine Fe 2+ transporter Fe 2+ pteridine cobalamin- formyltransferase, molybdopterin synthetase, pyridoxal aminotransferase: alcohol 5,7,24(28)-ergostatrienol like protein: G3IU-3075 hydrolase, thymidylate putative: G3IU-2316 kinase, synthase: INO1 cystathionine cystathione dehydrogenase- [+ 2 isozymes] deacetylase- (LIT1) reductase dependent kynurenine synthase] GTP putative: G3IU-1196 1D-myo-inositol (3) dehydrogenase, G3IU-5785 a 2,3,4- succinyl-coa:3- putative: synthase: α γ putative: beta-lyase, gamma like protein: like protein: 1: PTR1 methionine G3IU-6676 an -( -L- -monophosphate (S)-2-acetolactate putative: saturated (3S)-3- ketoacid- G3IU-3293 DHFR-TS L-γ- cysteinylglycine G3IU-3153 putative: lyase: G3IU-3953 G3IU-7785 dihydrofolate synthase: 2-nonaprenylphenol glutamyl)-L- indole-3-pyruvate G3IU-5139 hydroxyacyl-CoA coenzyme a dCDP pteridine glutamylcysteine myo-inositol-1 cystathionine G3IU-2276 G3IU-958 nitric oxide an oxidized reductase- G3IU-7803 amino acid (S)-3-hydroxy-3-methylglutaryl-CoA L-citrulline transferase- reductase: a thiocarboxylated pyridoxal-P phosphatase, alcohol 5,7,22,24(28)-ergostatetraenol beta-synthase: trifunctional tryparedoxin thymidylate [small subunit of dehydrogenase, CYSB cystathionine enzyme pyruvate a quinol like protein: D-ribose xanthine all folates PTR1 serine 3-hydroxy- cyclic pyranopterin putative: acetate L-citrulline synthase: molybdopterin putative putative: sterol C-24 beta-lyase- alpha subunit, G3IU-3076 hydroxymethyltransferase: L-kynurenine monophosphate gamma- G3IU-6523 3-hydroxy-3- cys citrate degradation DHFR-TS 2-nonaprenyl-6- synthase] pyruvate/ diacetyl (R)-acetoin G3IU-3057 reductase, like protein: mitochondrial SHMT-L 10-formyl- glutamylcysteine myo-inositol-1(or 4)- (R,R)-2,3- methylglutaryl-CoA 7,8-dihydrofolate kynureninase, hydroxyphenol