Electronic Supplementary Material (ESI) for ChemComm. This journal is © The Royal Society of Chemistry 2019 Table S4 Identified N-glycoproteins and N-glycopeptides sequence from the tryptic digest of proteins extracted from HepG2 cells after enrichment by AuGC/ZIF-8 Unique Protein Group No. Sequence Protein Descriptions Sequence Accessions Histone H2A type 1-D OS=Homo sapiens OX=9606 GN=HIST1H2AD PE=1 SV=2 - 1 KGNYSER 603 [H2A1D_HUMAN]|Histone H2A type 1-H OS=Mus musculus OX=10090 P20671;Q8CGP6 GN=Hist1h2ah PE=1 SV=3 - [H2A1H_MOUSE] Spectrin alpha chain, non-erythrocytic 1 OS=Homo sapiens OX=9606 GN=SPTAN1 2 KnNHHEENISSK 3137 Q13813 PE=1 SV=3 - [SPTN1_HUMAN] TATA-binding protein-associated factor 2N OS=Homo sapiens OX=9606 GN=TAF15 3 ENYSHHTQDDRR 3684 Q92804 PE=1 SV=1 - [RBP56_HUMAN] Pre-mRNA 3'-end-processing factor FIP1 OS=Homo sapiens OX=9606 GN=FIP1L1 4 NSTSSQSQTSTASR 3961 Q6UN15 PE=1 SV=1 - [FIP1_HUMAN] Nucleolar and coiled-body phosphoprotein 1 OS=Homo sapiens OX=9606 GN=NOLC1 5 NSSNKPAVTTK 4223 Q14978 PE=1 SV=2 - [NOLC1_HUMAN] 6 SGNLTEDDKHNNAK 4249 Plastin-3 OS=Homo sapiens OX=9606 GN=PLS3 PE=1 SV=4 - [PLST_HUMAN] P13797 Keratin, type I cytoskeletal 18 OS=Homo sapiens OX=9606 GN=KRT18 PE=1 SV=2 - 7 VVSETNDTK 5000 P05783 [K1C18_HUMAN] Osteopetrosis-associated transmembrane protein 1 OS=Homo sapiens OX=9606 8 AAGnTSESQScAR 5528 Q86WC4 GN=OSTM1 PE=1 SV=1 - [OSTM1_HUMAN] Transcription elongation factor A protein 1 OS=Homo sapiens OX=9606 GN=TCEA1 9 EESTSSGNVSNR 5530 P23193 PE=1 SV=2 - [TCEA1_HUMAN] Nuclear protein localization protein 4 homolog OS=Homo sapiens OX=9606 10 nKTGEITASSNK 5849 Q8TAT6 GN=NPLOC4 PE=1 SV=3 - [NPL4_HUMAN] Cysteine and glycine-rich protein 1 OS=Homo sapiens OX=9606 GN=CSRP1 PE=1 11 HEEAPGHRPTTNPNASK 6150 P21291 SV=3 - [CSRP1_HUMAN] Cytochrome b-c1 complex subunit 2, mitochondrial OS=Homo sapiens OX=9606 12 RGSNTTSHLHQAVAK 6163 P22695 GN=UQCRC2 PE=1 SV=3 - [QCR2_HUMAN] Aspartyl/asparaginyl beta-hydroxylase OS=Homo sapiens OX=9606 GN=ASPH PE=1 13 SSGNSSSSGSGSGSTSAGSSSPGAR 6247 Q12797 SV=3 - [ASPH_HUMAN] 14 LIDNnKTEK 6850 Prosaposin OS=Homo sapiens OX=9606 GN=PSAP PE=1 SV=2 - [SAP_HUMAN] P07602 Synaptic functional regulator FMR1 OS=Homo sapiens OX=9606 GN=FMR1 PE=1 15 TLqnTSSEGSR 6998 Q06787 SV=1 - [FMR1_HUMAN] Serpin H1 OS=Homo sapiens OX=9606 GN=SERPINH1 PE=1 SV=2 - 16 SLSnSTAR 7522 P50454 [SERPH_HUMAN] Laminin subunit beta-1 OS=Homo sapiens OX=9606 GN=LAMB1 PE=1 SV=2 - 17 LSDTTSQSnSTAK 7570 P07942 [LAMB1_HUMAN] Heterogeneous nuclear ribonucleoprotein D0 OS=Homo sapiens OX=9606 18 IDASKNEEDEGHSNSSPR 7623 Q14103 GN=HNRNPD PE=1 SV=1 - [HNRPD_HUMAN] 19 GGKnSTWSGESK 7930 Nucleolin OS=Homo sapiens OX=9606 GN=NCL PE=1 SV=3 - [NUCL_HUMAN] P19338 14-3-3 protein gamma OS=Homo sapiens OX=9606 GN=YWHAG PE=1 SV=2 - 20 NcSETQYESK 8385 P61981 [1433G_HUMAN] Ras-related protein Rab-5C OS=Homo sapiens OX=9606 GN=RAB5C PE=1 SV=2 - 21 NEPQNATGAPGR 8396 P51148 [RAB5C_HUMAN] Alpha-enolase OS=Homo sapiens OX=9606 GN=ENO1 PE=1 SV=2 - 22 KLNVTEQEK 8725 P06733 [ENOA_HUMAN] Peroxisomal multifunctional enzyme type 2 OS=Homo sapiens OX=9606 23 IDSEGGVSANHTSR 8823 P51659 GN=HSD17B4 PE=1 SV=3 - [DHB4_HUMAN] Heat shock cognate 71 kDa protein OS=Homo sapiens OX=9606 GN=HSPA8 PE=1 24 NQTAEKEEFEHQQK 8966 P11142 SV=1 - [HSP7C_HUMAN] 25 NSTWSGESK 9577 Nucleolin OS=Homo sapiens OX=9606 GN=NCL PE=1 SV=3 - [NUCL_HUMAN] P19338 Clathrin interactor 1 OS=Homo sapiens OX=9606 GN=CLINT1 PE=1 SV=1 - 26 ASPDQNASTHTPQSSVK 9983 Q14677 [EPN4_HUMAN] Triosephosphate isomerase OS=Pan troglodytes OX=9598 GN=TPI1 PE=2 SV=2 - 27 SNVSDAVAQSTR 10560 P60175 [TPIS_PANTR] Splicing factor U2AF 35 kDa subunit OS=Homo sapiens OX=9606 GN=U2AF1 PE=1 28 NPQNSSQSADGLR 10938 Q01081 SV=3 - [U2AF1_HUMAN] ATP-dependent RNA helicase DDX3X OS=Homo sapiens OX=9606 GN=DDX3X PE=1 SV=3 - [DDX3X_HUMAN]|ATP-dependent RNA helicase DDX3Y OS=Homo O00571;O15523; 29 VGSTSENITQK 10993 sapiens OX=9606 GN=DDX3Y PE=1 SV=2 - [DDX3Y_HUMAN]|ATP-dependent Q62167 RNA helicase DDX3X OS=Mus musculus OX=10090 GN=Ddx3x PE=1 SV=3 - [DDX3X_MOUSE] Eukaryotic translation initiation factor 3 subunit A OS=Homo sapiens OX=9606 30 KnLTQDEMQR 11412 Q14152 GN=EIF3A PE=1 SV=1 - [EIF3A_HUMAN] Torsin-1A-interacting protein 1 OS=Homo sapiens OX=9606 GN=TOR1AIP1 PE=1 31 SELGNQSPSTSSR 11515 Q5JTV8 SV=2 - [TOIP1_HUMAN] Tetraspanin-3 OS=Homo sapiens OX=9606 GN=TSPAN3 PE=2 SV=1 - 32 TYnGTNPDAASR 11449 O60637 [TSN3_HUMAN] Peptidyl-prolyl cis-trans isomerase FKBP10 OS=Homo sapiens OX=9606 GN=FKBP10 33 TLSRPSETcnETTK 11780 Q96AY3 PE=1 SV=1 - [FKB10_HUMAN] Hypoxia up-regulated protein 1 OS=Homo sapiens OX=9606 GN=HYOU1 PE=1 SV=1 34 nATLAEQAK 12139 - [HYOU1_HUMAN]|Hypoxia up-regulated protein 1 OS=Rattus norvegicus Q9Y4L1;Q63617 OX=10116 GN=Hyou1 PE=1 SV=1 - [HYOU1_RAT] SQNRPQGQSTQPSNAAGTNTTSAST Ubiquilin-2 OS=Homo sapiens OX=9606 GN=UBQLN2 PE=1 SV=2 - 35 12200 Q9UHD9 PR [UBQL2_HUMAN] Endoplasmic reticulum resident protein 29 OS=Homo sapiens OX=9606 GN=ERP29 36 QGQDNLSSVK 12464 P30040 PE=1 SV=4 - [ERP29_HUMAN] Proteasome subunit beta type-7 OS=Homo sapiens OX=9606 GN=PSMB7 PE=1 SV=1 37 ATEGMVVADKncSK 12876 Q99436 - [PSB7_HUMAN] Alpha-enolase OS=Homo sapiens OX=9606 GN=ENO1 PE=1 SV=2 - 38 LNVTEQEK 12916 P06733 [ENOA_HUMAN] L-lactate dehydrogenase A chain OS=Homo sapiens OX=9606 GN=LDHA PE=1 SV=2 39 DYNVTANSK 12961 P00338 - [LDHA_HUMAN] Eukaryotic translation initiation factor 3 subunit G OS=Homo sapiens OX=9606 40 VTNLSEDTR 13327 O75821 GN=EIF3G PE=1 SV=2 - [EIF3G_HUMAN] Tetraspanin-6 OS=Homo sapiens OX=9606 GN=TSPAN6 PE=1 SV=1 - 41 QYnSTGDYR 13432 O43657 [TSN6_HUMAN] Zinc finger CCCH domain-containing protein 4 OS=Homo sapiens OX=9606 42 TVNATGSSAAPGSSDKPSDPR 13618 Q9UPT8 GN=ZC3H4 PE=1 SV=3 - [ZC3H4_HUMAN] Myristoylated alanine-rich C-kinase substrate OS=Homo sapiens OX=9606 43 AEDGATPSPSNETPK 12056 P29966 GN=MARCKS PE=1 SV=4 - [MARCS_HUMAN] Chromobox protein homolog 5 OS=Homo sapiens OX=9606 GN=CBX5 PE=1 SV=1 - 44 KSNFSNSADDIK 14211 P45973 [CBX5_HUMAN] Alpha-actinin-1 OS=Homo sapiens OX=9606 GN=ACTN1 PE=1 SV=2 - [ACTN1_HUMAN]|Alpha-actinin-2 OS=Homo sapiens OX=9606 GN=ACTN2 PE=1 45 HTnYTMEHIR 9176 P12814;O43707 SV=1 - [ACTN2_HUMAN]|Alpha-actinin-4 OS=Homo sapiens OX=9606 GN=ACTN4 PE=1 SV=2 - [ACTN4_HUMAN] E3 SUMO-protein ligase RanBP2 OS=Homo sapiens OX=9606 GN=RANBP2 PE=1 46 SNNSETSSVAQSGSESKVEPK 14833 P49792 SV=2 - [RBP2_HUMAN] Tryptophan--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=WARS PE=1 47 AGNASKDEIDSAVK 15074 P23381 SV=2 - [SYWC_HUMAN] Alpha-enolase OS=Homo sapiens OX=9606 GN=ENO1 PE=1 SV=2 - 48 KLNVTEQEKIDK 15155 P06733 [ENOA_HUMAN] DNA replication licensing factor MCM7 OS=Homo sapiens OX=9606 GN=MCM7 49 MNKSEDDESGAGELTR 15223 P33993 PE=1 SV=4 - [MCM7_HUMAN] Cytokine receptor-like factor 1 OS=Homo sapiens OX=9606 GN=CRLF1 PE=1 SV=1 - 50 VVDDVSnqTScR 15229 O75462 [CRLF1_HUMAN] E3 SUMO-protein ligase RanBP2 OS=Homo sapiens OX=9606 GN=RANBP2 PE=1 51 QNQTTSAVSTPASSETSK 15234 P49792 SV=2 - [RBP2_HUMAN] Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS 52 AIQGGTSHHLGQNFSK 15678 P07814 PE=1 SV=5 - [SYEP_HUMAN] Splicing factor 1 OS=Homo sapiens OX=9606 GN=SF1 PE=1 SV=4 - 53 LnGTLREDDNR 15724 [SF01_HUMAN]|Splicing factor 1 OS=Mus musculus OX=10090 GN=Sf1 PE=1 SV=6 Q15637;Q64213 - [SF01_MOUSE] Vacuole membrane protein 1 OS=Homo sapiens OX=9606 GN=VMP1 PE=1 SV=1 - 54 EHHnGnFTDPSSVNEK 15998 Q96GC9 [VMP1_HUMAN] Alpha-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=NAGLU PE=1 SV=2 55 SVYncSGEAcR 16023 P54802 - [ANAG_HUMAN] Tissue factor pathway inhibitor OS=Homo sapiens OX=9606 GN=TFPI PE=1 SV=1 - 56 YSGcGGnENNFTSK 16293 P10646 [TFPI1_HUMAN] Thrombospondin-1 OS=Homo sapiens OX=9606 GN=THBS1 PE=1 SV=2 - 57 VVnSTTGPGEHLR 16418 P07996 [TSP1_HUMAN] 60 kDa heat shock protein, mitochondrial OS=Homo sapiens OX=9606 GN=HSPD1 58 VTDALNATR 16708 P10809 PE=1 SV=2 - [CH60_HUMAN] 59 ALEENnNFSK 16910 Stathmin OS=Homo sapiens OX=9606 GN=STMN1 PE=1 SV=3 - [STMN1_HUMAN] P16949 60 AIEENnNFSK 16910 Stathmin OS=Homo sapiens OX=9606 GN=STMN1 PE=1 SV=3 - [STMN1_HUMAN] P16949 Histone H1.5 OS=Homo sapiens OX=9606 GN=HIST1H1B PE=1 SV=3 - 61 KALAAGGYDVEKnNSR 17035 P16401 [H15_HUMAN] L-lactate dehydrogenase A chain OS=Homo sapiens OX=9606 GN=LDHA PE=1 SV=2 62 IVSGKDYNVTANSK 17049 P00338 - [LDHA_HUMAN] Nuclear pore complex protein Nup88 OS=Homo sapiens OX=9606 GN=NUP88 PE=1 63 STVNcSTTPVAER 17143 Q99567 SV=2 - [NUP88_HUMAN] Regulator of nonsense transcripts 1 OS=Homo sapiens OX=9606 GN=UPF1 PE=1 64 ESQTQDNITVR 17230 Q92900 SV=2 - [RENT1_HUMAN] Histone H1.2 OS=Homo sapiens OX=9606 GN=HIST1H1C PE=1 SV=2 - 65 KALAAAGYDVEKNNSR 17355 [H12_HUMAN]|Histone H1.4 OS=Homo sapiens OX=9606 GN=HIST1H1E PE=1 P16403;P10412 SV=2 - [H14_HUMAN] Inactive tyrosine-protein kinase 7 OS=Homo sapiens OX=9606 GN=PTK7 PE=1 SV=2 66 DGTPLSDGqSNHTVSSK 17488 Q13308 - [PTK7_HUMAN] 67 DVEcGEGHFcHDnQTccR 17844 Progranulin OS=Homo sapiens OX=9606 GN=GRN PE=1 SV=2 - [GRN_HUMAN] P28799 Retrotransposon-derived protein PEG10 OS=Homo sapiens OX=9606 GN=PEG10 68 LTEENTTLR 18072 Q86TG7 PE=1 SV=2 - [PEG10_HUMAN] 60S ribosomal protein L26 OS=Macaca fascicularis OX=9541 GN=RPL26 PE=2 SV=1 69 AnGTTVHVGIHPSK 18248 P61256 - [RL26_MACFA] T-complex protein 1 subunit beta OS=Homo sapiens OX=9606 GN=CCT2 PE=1 SV=4 70 AAHSEGNTTAGLDMR 18303 P78371 - [TCPB_HUMAN] Keratin, type I cytoskeletal 18 OS=Homo sapiens OX=9606 GN=KRT18 PE=1 SV=2 - 71 KVIDDTNITR 18368 P05783 [K1C18_HUMAN] HLA class I histocompatibility antigen, A-2 alpha chain OS=Homo sapiens OX=9606 72 GYYnQSEAGSHTVQR 18527 P01892 GN=HLA-A PE=1 SV=1 - [1A02_HUMAN] Transferrin receptor protein 1 OS=Homo