LECTURE PROGRAMME Tuesday, April 24, 2012 13:30 Welcome Address A. Liese, Hamburg University of Technology/D Chair: C. Syldatk, Karlsruhe Institute of Technology/D Page 13:40 Keynote Lecture 1 Industrial Biotechnology: Directing Innovation in Chemistry B. Hauer, University of Stuttgart/D 14:10 Eco-efficient synthesis of chiral alcohols by combining 2 biocatalysis with "Smart Cosubstrates" S. Kara. Delft University of Technology, Delft/NL, D. Spickermann, C. Leggewie, evocatal GmbH, Duesseldorf/D, I.W.C.E. Arends, F. Hollmann, Delft University of Technology, Delft/NL 14:30 Phytotherapies production from olive mill waste water by 3 intensified biocatalytic membrane reactor R. Mazzei. E. Drioli, L. Giorno, Institute on Membrane Technology, Rende/I 14:50 Substrate delivery strategies for improved enzymatic 5 carotenoid cleavage C. Nacke. S. Huttmann, M. Schilling, J. Schrader, DECHEMA Research Institute, Frankfurt am Main/D 15:10 Coffee Break and Poster Viewing i http://d-nb.info/102199667X LECTURE PROGRAMME Tuesday, April 24, 2012 Chair: J. Schrader, DECHEMA Research Institute, Frankfurt am Main/D 15:40 Enzyme-catalyzed fine-tuning of the molecular weight 6 distribution of cellulose B. Lammerhirt. Hamburg University of Technology/D, A. Prediger, Leibniz University Hanover/D, M. Eckstein, Evonik Industries AG, Essen/D, L. Hilterhaus, Hamburg University of Technology/D , S. Beutel, T. Scheper, Leibniz University Hanover/D, A. Liese, Hamburg University of Technology/D 16:00 Poster Speed Lectures Part I Topic 1: New reactions 24 - 30 Topic 2: Smart screenings for the ideal biocatalyst 31-34 Topic 3: Overcoming substrate and product inhibitions 35 - 36 Topic 4: Unconventional reaction systems 37 - 43 17:00 Coffee break and poster viewing Chair: A. Liese, Hamburg University of Technology/D 17:20 Novel biocatalysts for the hydrolysis of diketopiperazines M. Perzborn. C. Syldatk, J. Rudat, Karlsruhe Institute of Technology/D 17:40 Poster Speed Lectures Part II Topic 5: Taking advantage of enzyme promiscuity 44 - 49 Topic 6: Enzyme stabilization and immobilization 50 - 58 Topic 7: Enzyme reaction cascades 59 - 63 19:00 Poster Session and Get-together 22:00 LECTURE PROGRAMME Wednesday, April 25, 2012 Chair: A. Schmid, TU Dortmund University/D Page 08:30 Engineering the thermostability of sucrose phosphorylase 8 K. De Winter. T. Desmet, A. Cerdobbel, W. Soetaert, Ghent University/B 08:50 Immobilization of enzymes on textile carrier materials 9 K. Qpwis. J.S. Gutmann, Deutsches Textilforschungszentrum Nord-West e.V., Krefeld/D 09:10 Immobilization of beta-galactosidase on membranes for 10 process intensification Y. Satvawali. S. Van Roy, Flemish Institute for Technological Research, Mol/B, P. Jochems, L. Diels, Flemish Institute for Technological Research, Mol/B, University of Antwerp, Antwerp/B, W. Dejonghe, Flemish Institute for Technological Research, Mol/B 09:30 Robust enzyme preparations for stereoselective reduction 11 A. Scholz , M. Ansorge-Schumacher, Technical University of Berlin, Berlin/D, M. Eckstein, Evonik lndustries AG, Essen/D 09:50 Combining molecular engineering andxovalent immobiliza- 12 tion for a compartmented enzymatic reaction sequence J. Simons. Hamburg University of Technology/D, M. Mosisch, A. Torda, University of Hamburg/D, L. Hilterhaus, Hamburg University of Technology/D 10:10 Coffee break and poster viewing LECTURE PROGRAMME Wednesday, April 25, 2012 Chair: J. Eck, BRAIN AG, Zwingenberg/D Page 10:40 Keynote Lecture 13 In silico design and engineering of biocatalysts for industrial biotechnology J. Damborsky, Masaryk University Kamenice, Bmo/CZ 11:10 Identification of rare aspartase variants that catalyze 14 beta-amino acid synthesis R. Schmiedel. A. Vogel, c-LEcta GmbH, Leipzig/D 11.30 Synthesis of omega-hydroxy fatty acids by bacterial 15 CYP153A monooxygenases S. Honda Malca. D. Scheps, L. Kuhnel, E. Venegas-Venegas, A. Seifert, B. M. Nestl, B. Hauer, University of Stuttgart/D 11:50 Efficient route to selective bio-oxidation catalysts: 16 Iterative approach comprising modeling, diversification and screening A. Seifert. M. Antonovici, B. Hauer, J. Pleiss, University of Stuttgart/D 12:10 Lunch break LECTURE PROGRAMME Wednesday, April 25, 2012 Chair: B. Hauer, Universitat Stuttgart/D Page 13:10 NAD(P)H-independent asymmetric C=C-bond reduction by 17 ene-reductases: Strategies to overcome co-product inhibi­ tion C. K. Winkler. D. Clay, G. Tasnadi, M. Hall, K. Faber, University of Graz/A 13:30 Novel whole-ceil biocataiysts for the reduction of 18 dehydrocholic acid using hydroxysteroid dehydrogenases with reduced substrate inhibition. B. Sun. M. Braun, D. Weuster-Botz, Technische Universitat Munchen, Garching/D 13:50 Keynote Lecture 19 Biocatalysis goes multistep: From natural biodiversity to synthetic designer bugs J. Eck, BRAIN AG, Zwingenberg/D 14:20 Coffee break and poster viewing Chair: L. Wessjohann, Leibniz Institute of Plant Biochemistry, Page Halle/D 14:50 Substrate promiscuity of a plant peroxygenase 20 C. Fuchs. W. Schwab, Technische Universitat Munchen, Freising/D 15:10 Metal-mediated promiscuous enzyme activity 21 N.R. Uda, M. Creus. University of Basel/CH 15:30 Promiscuity of a novel lipoxygenase (LOX) from Pleurotus 22 sapidus I. Plaaemann. K. Zelena, U. Krings, R. G. Berger, University of Hanover/D 15:50 Engineering the promiscuous racemising activity of arylma- 23 lonate decarboxylase from Bordatella bronchoseptica R. Kourist. Ruhr-Universitat Bochum/D, Y. Miyauchi, K. Miyamoto, Keio University, Yokohama/J 16:10 Closing remarks Poster Programme No. Topic 1: New Reactions Page P01 Development of tereocomplementary sec-alkylsulfatases 24 M. Schober. M. Toesch, University of Graz/A, T. Knaus, P. Macheroux, Graz University of Technology/A, U. Wagner, K. Faber, University of Graz/A P02 Yeast as a whole-cell biocatalyst for the selective oxidation 25 of fatty acids J. C. Guder. M. Buchhaupt, J. Schrader, DECHEMA Research Institute, Frankfurt am Main/D P03 A recombinant a-dioxygenase from rice to produce fatty 26 aldehydes using E. coli M. Buchhauot. F. Sporleder, J. Schrader, DECHEMA Research Institute, Frankfurt am Main/D P04 MenD as an unusual thiamine-diphosphate-dependent 27 biocatalyst: 1,2- and 1,4-additions M. Beigi, A. Kurutsch, University of Freiburg/D, S. Baier, G. A. Sprenaer. University of Stuttgart/D, M. Muller, University of Freiburg/D P05 Substrate specificity of a novel squalene-hopene cyclase 28 from Zymomonas mobilis M. Seitz. S. Hammer, J. Klebensberger, B. Nestl, P.-O. Syr6n, Universitat Stuttgart/D, M. Breuer, BASF SE, Ludwigshafen/D, B. Hauer, Universitat Stuttgart/D P06 Investigation of reaction parameters influencing selectivity in 29 the synthesis of chiral hydroxy ketones with carbonyl reductases C. Loderer. M. Ansorge-Schumacher, TU Berlin/D P07 Cytochrome P450 reductase from Candida apicola: Versatile 30 redox partner for bacterial P450s F. Tieves. M. Girhard, University of Dusseldorf/D; E. Weber, Universitat Stuttgart/D; M.S. Smit, University of the Free State, Bloemfontein/ZA; V.B. Urlacher, University of Dusseldorf/D Poster Programme No. Topic 2: Smart Screenings for the Ideal Biocatalyst Page P08 Enzymes catalyzing asymmetric carboligation: Directed 31 evolution of substrate scope towards potential industrial applications D. Yi. T. Devamani, W.- D. Fessner, TU Darmstadt/D P09 A microtiter plate based technique for the fast optimization 32 of electroenzymatic processes C. Lev. D. Holtmann, J. Schrader, DECHEMA Research Institute, Frankfurt am Main/D P10 Enzymatic modification of microbial alkyl-sophorosides 33 V. K. Recke. TU Braunschweig/D, M. Gerlitzki, R. Hausmann, C. Syldatk, Karlsruhe Institute of Technology (KIT)/D, V. Wray, Helmholtz Centre of Infection Research, Braunschweig/D, H. Tokuda, Kanazawa University/J, S. Lang, TU Braunschweig/D P11 Biocatalytic whole-cetl-screening in microwell plates: 34 Determination of the best growth and screening-system for yeast reduction processes T. Grimm. Technische Universitat Berlin/D, BIOWORX Biotechno- logielabor Thomas Grimm, Berlin/D, R. Roller, Technische Universitat Berlin/D, A. Neubauer, BioSilta Oy, Oulu/FI, P. Neubauer, Technische Universitat Berlin/D No. Topic 3: Overcoming Substrate and Product Inhibitions Page P12 Microbial conversion of (±)-linalool to linalool oxides by 35 Corynespora cassiicola M.M.W. Etschmann. S. Bormann, M.A. Mirata, J. Schrader, DECHEMA Research Institute, Frankfurt am Main/D P13 Resting cells of recombinant E. coli show high epoxidation 36 yields on energy source and high sensitivity to product inhi­ bition M.K. Julsina. D. Kuhn, A. Schmid, B. Buhler, TU Dortmund/D Poster Programme No. Topic 4: Unconventional Reaction Systems Page P14 Halophilic bacteria as a source for salt-tolerating cellulases 37 and xylanases N. Staiaer. T. Frey, Fraunhofer Institute for Interfacial Engineer­ ing and Biotechnology IGB, Stuttgart/D, T. Hirth, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart/D, Institute for Interfacial Engineering, University of Stuttgart/D, S. Rupp, S. Zibek, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart/D P15 Expression of recombinant human flavin monooxygenases 38 and application for N-oxidation of moclobemide A. Glieder. acib GmbH, Graz/A, A. Camattari, University of Technology, Graz/A, T. Bachler, M. Winkler, acib GmbH, Graz/A P16 Artificial fusion of a two-component styrene monooxygenase 39 D. Tischler. T. Heine, C. Conrad, J. A. D. Groning, S. R. Kaschabek, M. Schlomann, TU Bergakademie Freiberg/D P17 Synthesis