http://www.chemistryexplained.com/images/chfa_04_img0954.jpg http://www.bio-world.com/images/reagents.jpg http://www.nlm.nih.gov/visibleproofs/media/detailed/iii_d_142b.jpg Radioimmunoassay 2310302 RAD TRACER Lerson Tanasugarn, Ph.D. Department of Biochemistry, Faculty of Science, Chulalongkorn University Klum 515, Phone 662-218-5424, Departmental FAX 662-218-5418 Email: [email protected] Handout: [email protected]/public/biochemistry/2010 2310302 RIA.pdf Copyright © 2010 by Lerson Tanasugarn. All rights reserved. 1 Contents • History of radioimmunoassay • Principles of radioimmunoassay • Non-isotope immunoassay • Methods • Applications Copyright © 2010 by Lerson Tanasugarn. All rights reserved. 2 History http://2.bp.blogspot.com/ http://www.aps.org/publications/ Rall, J. E. 1990. Solomon A. http://www.invention-ifia.ch/ _DZH2cmCoois/SLFpb8J_GPI/ apsnews/199810/images/ Berson 1918-1972. National images/Rosalyn_Yalow2.jpg AAAAAAAAFvc/4fpGNpLZ6YE/s400/ rosalyn_sussman_yalow.jpg Academy of Sciences. Nobel_Laureate_1977_Rosalyn_Yalow .bmp Rosalyn Sussman Yalow 1921- Solomon Berson 1918 - 1972 • City College of NY - BS and MSc • Born in NYC (NY+German) • Anatomy instructorship NYU • Hunter College BS • MD (NYU 1945) • PhD in physics (U. Illinois 1945) • Intern (Boston City Hospital 1945-46) • Joined the Bronx V.A. Hospital to set up • US Army (1946-48) its radioisotope service • Recruited by Yalow to work in the Radioisotope • Recruit Berson to form a research team Service of the Bronx V.A. Hospital (to support her work) • Chief of Radioisotope Service (forerunner of the Nuclear Medicine Service) 1954 • Rall, J. E. 1990. Solomon A. Berson 1918-1972 A Biographical Memoir. National Academy of Sciences Washington, D.C. http://books.nap.edu/html/biomems/sberson.pdf • Yalow, R. 1977. Autobiography, http://nobelprize.org/nobel_prizes/medicine/laureates/1977/yalow-autobio.html • Yalow, Rosalyn S. 1999. Development and proliferation of radioimmunoassay technology. J. Chem. Edu. 76(6):767-768. Copyright © 2010 by Lerson Tanasugarn. All rights reserved. 3 1951 - isotope & blood volume from Biography of Sol Berson His initial publicationsSOLOMO (1951-1960)N A. BERSON are listed below63 SELECTED BIBLIOGRAPHY 1951 With R. S. Yalow. The use of K42-tagged erythrocytes in blood vol- ume determinations. Science, 114:14-15. • isotope dilution technique1952 used for blood volume determinationWith R. S. Yalow. The effect of cortisone on the iodine accumulat- ing function of the thyroid gland in euthyroid subjects. J. Clin. Endocrinol. Metab., 12:407-22. With R. S. Yalow, J. Sorrentino, and B. Roswit. The determination 1S1 of thyroidal and renal plasma I clearancCopyright © e2010 rate by Lersons a Tanasugarn.s a routin All rights ereserved. 4 diagnostic test of thyroid dysfunction. J. Clin. Invest., 31:141— 58. With R. S. Yalow. The use of K42 or P32 labeled erythrocytes and I131 tagged human serum albumin in simultaneous blood vol- ume determinations. J. Clin. Invest., 31:572-80. With R. S. Yalow, A. Azulay, S. Schreiber, and B. Roswit. The bio- logical decay curve of P32-tagged erythrocytes. Application to the study of acute changes in blood volume. J. Clin. Invest., 31:581-91. 1953 With R. S. Yalow, J. Post, L. H. Wisham, K. N. Newerly, M. J. Vil- lazon, and O. N. Vazquez. Distribution and fate of intravenously administered modified human globin and its effect on blood volume. Studies utilizing I131-tagged globin. J. Clin. Invest., 32:22-32. With R. S. Yalow, S. S. Schreiber, and J. Post. Tracer experiments with I131-labeled human serum albumin: Distribution and deg- radation studies. J. Clin. Invest., 32:746-68. 1954 With R. S. Yalow. The distribution of I131-labeled human serum albumin introduced into ascitic fluid: Analysis of the kinetics of a three compartment catenary transfer system in man and spec- ulations on possible sites of degradation. J. Clin. Invest., 33:377-87. With S. S. Schreiber, A. Bauman, and R. S. Yalow. Blood volume SOLOMON A. BERSON 63 SELECTED BIBLIOGRAPHY 1951 With R. S. Yalow. The use of K42-tagged erythrocytes in blood vol- 1952ume determinations - radioisotope. Science, 114:14-15 iodine. exchange 1952 With R. S. Yalow. The effect of cortisone on the iodine accumulat- ing function of the thyroid gland in euthyroid subjects. J. Clin. Endocrinol. Metab., 12:407-22. With R. S. Yalow, J. Sorrentino, and B. Roswit. The determination of thyroidal and renal plasma I1S1 clearance rates as a routine diagnostic test of thyroid dysfunction. J. Clin. Invest., 31:141— 58. With R. S. Yalow. The use of K42 or P32 labeled erythrocytes and I131 tagged human serum albumin in simultaneous blood vol- ume determinations. J. Clin. Invest., 31:572-80. With R. S. Yalow, A. Azulay, S. Schreiber, and B. Roswit. The bio- logical decay curve of P32-tagged erythrocytes. Application to the study of acute changes in blood volume. J. Clin. Invest., 31:581-91. • uptake of radioactive 195iodine3 by thyroid With R. S. Yalow, J. Post, L. H. Wisham, K. N. Newerly, M. J. Vil- lazon, and O. N. Vazquez. Distribution and fate of intravenously administered modified human globin and its effect on blood volume. Studies utilizing I131-tagged globin. J. Clin. Invest., 32:22-32. Copyright © 2010 by Lerson Tanasugarn. All rights reserved. 5 With R. S. Yalow, S. S. Schreiber, and J. Post. Tracer experiments with I131-labeled human serum albumin: Distribution and deg- radation studies. J. Clin. Invest., 32:746-68. 1954 With R. S. Yalow. The distribution of I131-labeled human serum albumin introduced into ascitic fluid: Analysis of the kinetics of a three compartment catenary transfer system in man and spec- ulations on possible sites of degradation. J. Clin. Invest., 33:377-87. With S. S. Schreiber, A. Bauman, and R. S. Yalow. Blood volume SOLOMON A. BERSON 63 SELECTED BIBLIOGRAPHY 1951 With R. S. Yalow. The use of K42-tagged erythrocytes in blood vol- ume determinations. Science, 114:14-15. 1952 With R. S. Yalow. The effect of cortisone on the iodine accumulat- ing function of the thyroid gland in euthyroid subjects. J. Clin. Endocrinol. Metab., 12:407-22. With R. S. Yalow, J. Sorrentino, and B. Roswit. The determination of thyroidal and renal plasma I1S1 clearance rates as a routine diagnostic test of thyroid dysfunction. J. Clin. Invest., 31:141— 58. With R. S. Yalow. The use of K42 or P32 labeled erythrocytes and I131 tagged human serum albumin in simultaneous blood vol- ume determinations. J. Clin. Invest., 31:572-80. With R. S. Yalow, A. Azulay, S. Schreiber, and B. Roswit. The bio- logical decay curve of P32-tagged erythrocytes. Application to 1953 th-e studradioiodiney of acute changes in bloo labelingd volume. J. Clin .of Invest. proteins, 31:581-91. 1953 With R. S. Yalow, J. Post, L. H. Wisham, K. N. Newerly, M. J. Vil- lazon, and O. N. Vazquez. Distribution and fate of intravenously administered modified human globin and its effect on blood volume. Studies utilizing I131-tagged globin. J. Clin. Invest., 32:22-32. With R. S. Yalow, S. S. Schreiber, and J. Post. Tracer experiments with I131-labeled human serum albumin: Distribution and deg- radation studies. J. Clin. Invest., 32:746-68. 1954 With R. S. Yalow. The distribution of I131-labeled human serum albumin introduced into ascitic fluid: Analysis of the kinetics of a three compartment catenary transfer system in man and spec- ulations on possible sites of degradation. J. Clin. Invest., 33:377-87. With S. S. Schreiber, A. Bauman, and R. S. Yalow. Blood volume • plasma protein labeled with radioactive iodine for studying the metabolism of these proteins Copyright © 2010 by Lerson Tanasugarn. All rights reserved. 6 64 BIOGRAPHICAL MEMOIRS alterations in congestive heart failure. J. Clin. Invest., 33:578- 86. With R. S. Yalow. Quantitative aspects of iodine metabolism. The exchangeable organic iodine pool, and the rates of thyroidal secretion, peripheral degradation and fecal excretion of endog- enously synthesized organically bound iodine. J. Clin. Invest., 33:1533-52. 1955 With R. S. Yalow. Critique of extracellular space measurements with small ions: Na24 and Br82 spaces. Science, 121:34-36. With R. S. Yalow. The iodide trapping and binding functions of the thyroid. J. Clin. Invest., 34:186-204. With M. A. Rothschild, A. Bauman, and R. S. Yalow. Tissue distri- bution of I131-labeled human serum albumin following intra- venous administration. J. Clin. Invest., 34:1354-58. With A. Bauman, M. A. Rothschild, and R. S. Yalow. Distribution and metabolism of I131-labeled human serum albumin in congestive heart failure with and without proteinuria. J. Clin. Invest., 34:1359-68. 1956 With R. S. Yalow, A. Bauman, M. A. Rothschild, and K. Newerly. Insulin-I131 metabolism in human subjects: demonstration of 1953-59 insulin binding globulin in the circulation of insulin-treated subjects. J. Clin. Invest., 35:170-90. •retarded production of insulin? 1957 excessively rapid degradation of normally secreted insulin? • With R. S. Yalow. Chemical and biologicalmaturity-onset alterations induced by •too few or defective insulin receptors?irradiatio n of I131-labeled human serum albumin. J. Clin. In- •abnormality in the insulin responsevest. pathway, 36:44-50 inside. the cells? diabetes With M. A. Rothschild, A. Bauman, and R. S. Yalow. The effect of used synthesized labeledlarge doses o finsulin desiccated thyroito followd on the distributio degradationn and me- tabolism of albumin-I1M in euthyroid subjects. J. Clin. Invest., 131 36:422-28Insulin-I. Insulin-I131 slowly disappears due to Normal SubjectWith R. S. Yalow. Serum