Roskilde University Daphnia Halloween genes that encode cytochrome P450s mediating the synthesis of the arthropod molting hormone Evolutionary implications Rewitz, Kim; Gilbert, Lawrence I. Published in: BMC Evolutionary Biology DOI: 10.1186/1471-2148-8-60 Publication date: 2008 Document Version Publisher's PDF, also known as Version of record Citation for published version (APA): Rewitz, K., & Gilbert, L. I. (2008). Daphnia Halloween genes that encode cytochrome P450s mediating the synthesis of the arthropod molting hormone: Evolutionary implications. 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Sep. 2021 BMC Evolutionary Biology BioMed Central Research article Open Access Daphnia Halloween genes that encode cytochrome P450s mediating the synthesis of the arthropod molting hormone: Evolutionary implications Kim F Rewitz1 and Lawrence I Gilbert*2 Address: 1Department of Science, Systems and Models, Roskilde University, Postbox 260, DK-4000 Roskilde, Denmark and 2Department of Biology, University of North Carolina, Chapel Hill, NC 27599-3280 USA Email: Kim F Rewitz - [email protected]; Lawrence I Gilbert* - [email protected] * Corresponding author Published: 25 February 2008 Received: 15 November 2007 Accepted: 25 February 2008 BMC Evolutionary Biology 2008, 8:60 doi:10.1186/1471-2148-8-60 This article is available from: http://www.biomedcentral.com/1471-2148/8/60 © 2008 Rewitz and Gilbert; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: In crustaceans and insects, development and reproduction are controlled by the steroid hormone, 20-hydroxyecdysone (20E). Like other steroids, 20E, is synthesized from cholesterol through reactions involving cytochrome P450s (CYPs). In insects, the CYP enzymes mediating 20E biosynthesis have been identified, but evidence of their probable presence in crustaceans is indirect, relying solely on the ability of crustaceans to synthesize 20E. Results: To investigate the presence of these genes in crustaceans, the genome of Daphnia pulex was examined for orthologs of these genes, the Halloween genes, encoding those biosynthetic CYP enzymes. Single homologs of spook-CYP307A1, phantom-CYP306A1, disembodied-CYP302A1, shadow- CYP315A1 and shade-CYP314A1 were identified in the Daphnia data base. Phylogenetic analysis indicates an orthologous relationship between the insect and Daphnia genes. Conserved intron/ exon structures and microsynteny further support the conclusion that these steroidogenic CYPs have been conserved in insects and crustaceans through some 400 million years of evolution. Conclusion: Although these arthropod steroidogenic CYPs are related to steroidogenic CYPs in Caenorhabditis elegans and vertebrates, the data suggest that the arthropod steroidogenic CYPs became functionally specialized in a common ancestor of arthropods and are unique to these animals. Background a specific biosynthetic pathway yielding 20-hydroxyecdys- Steroid hormones, regulate essential processes during one (20E), the arthropod molting hormone, evolved, development and reproduction, and are synthesized from whereas in the line leading to vertebrates, biosynthetic cholesterol under the control of steroidogenic enzymes in CYPs that produce the vertebrate-type steroids evolved the cytochrome P450 (CYP) family [1]. In Caenorhabditis [2]. Since there is some evidence of the presence of verte- elegans, insects and vertebrates, different steroids are pro- brate-type sex steroids in invertebrates such as echino- duced to control developmental processes, suggesting that derms and mollusks, although no unequivocal evidence steroidogenic CYPs evolved and became functionally spe- that they can synthesize these steroids [3], the possibility cialized in different lineages during evolution. In insects, remains that CYPs with the capacity to produce vertebrate- Page 1 of 8 (page number not for citation purposes) BMC Evolutionary Biology 2008, 8:60 http://www.biomedcentral.com/1471-2148/8/60 type sex steroids were present in the common ancestor Results and Discussion even before the protostome-deuterostome split. Thus, the By searching the Daphnia data bases [18,19] we obtained evolution of steroidogenic CYPs is still an open question. candidate sequences for orthologs of the insect Halloween genes in Daphnia. Single orthologs of phm, dib, sad and shd Crustaceans are believed to represent the ancestral arthro- were retrieved and only one sequence exhibited signifi- pods from which insects originated [4]. The evolutionary cant similarity to the spo-like genes in the CYP307 family. relationship between these two groups is evident from the We also searched the genomes of non-arthropod inverte- common growth strategy of insects and crustaceans that brates including the cnidarian Nematostella vectensis, the involves molting so that growth can occur. Molting is gov- nematodes C. elegans and Brugia malayi, the annelid erned by periodic increases in the levels of 20E that elicit Capitella capitata, the mollusk Lottia gigantea, the echino- the programs that coordinate the developmental and derm Strongylocentrotus purpuratus [19,20] for Halloween metamorphic transitions [5]. Although a great deal of evi- gene orthologs using TBLASTN. Analyses of these inverte- dence reveals that crustaceans, like insects, synthesize 20E brate genomes did not result in any significant hits indi- from cholesterol [6], the molecular details of steroidogen- cating the absence of Halloween orthologs and neither esis in crustaceans remain conjectural. In insects, ster- have we been able to identify orthologs of these genes in oidogenic CYPs are products of the Halloween genes any of several vertebrate species. phantom (phm: CYP306A1), disembodied (dib: CYP302A1), shadow (sad: CYP315A1) and shade (shd: CYP314A1) and The genes obtained from Daphnia encode approximately are responsible for the last four hydroxylations in the 500 amino acid open reading frames (ORFs) which is typ- pathway leading to 20E [7-14] that is biochemically simi- ically for proteins belonging to the CYP family [21]. Align- lar to one that yields 20E in crustaceans [6] (Fig. 1). In ment of Daphnia sequences with orthologs of the insect Drosophila melanogaster, mutations in these genes disrupt Halloween genes shows that the genes, in addition to 20E production and cause the arrest of embryonic devel- being conserved in areas that comprise canonical struc- opment and death. spook (spo: Cyp307a1) is another mem- tural CYP motifs, exhibit considerable conservation in ber of this CYP group which when mutated results in low regions that are believed to determine substrate specificity 20E mutants [15,16] and is believed to mediate a yet (Additional file 1). This indicates that the genes are func- uncharacterized step (the Black Box) in the biosynthesis tionally conserved in Daphnia as they are in insects [2]. of 20E preceding those of Phm, Dib, Sad and Shd. In con- The overall amino acid identity between deduced orthol- trast to phm, dib, sad and shd for which each insect genome ogous proteins from eight insects belonging to four differ- carries one ortholog, several paralogs of spo-like (CYP307) ent orders and the Daphnia orthologs ranges from an genes have been formed by duplications, which in turn average of 55.2% ± 6.8 SD (standard deviation) for Spo- have evolved lineage-specific complements of these genes like proteins to somewhat lower values for Sad proteins [2,16,17]. For example, Drosophila has two spo-like genes, (38.7% ± 8.8 SD). Thus, spo-like genes are the most highly spo and spookier (spok: Cyp307a2) [16]. These two genes are conserved of these genes and this appears to be true for close paralogs that are believed to mediate the same enzy- Daphnia like it is for insects. Conservation of these genes matic reaction, although at different stages of develop- from insect to crustaceans, which separated ~400 millions ment. years ago [4], shows that selection has preserved the genes because of their function. The reason that spo-like genes Although one would expect that orthologs of the insect are more conserved than the other arthropod steroidog- Halloween genes are present in crustaceans, there is no enic CYP enzymes is not known, although it may be molecular evidence for the existence of these genes in related to the possibility that Spo acts in the rate-limiting crustaceans. We have tried for several years to probe hexa- Black Box reaction(s) [5,22]. If Spo is involved in control