University of Kentucky UKnowledge Plant and Soil Sciences Faculty Patents Plant and Soil Sciences 5-21-2013 Cytochrome P450s and Uses Thereof Joe Chappell University of Kentucky, [email protected] Lyle F. Ralston Right click to open a feedback form in a new tab to let us know how this document benefits oy u. Follow this and additional works at: https://uknowledge.uky.edu/pss_patents Part of the Plant Sciences Commons Recommended Citation Chappell, Joe and Ralston, Lyle F., "Cytochrome P450s and Uses Thereof" (2013). Plant and Soil Sciences Faculty Patents. 12. https://uknowledge.uky.edu/pss_patents/12 This Patent is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in Plant and Soil Sciences Faculty Patents by an authorized administrator of UKnowledge. For more information, please contact [email protected]. USOO8445231B2 (12) United States Patent (10) Patent N0.2 US 8,445,231 B2 Chappell et al. (45) Date of Patent: May 21, 2013 (54) CYTOCHROME P4505 AND USES THEREOF 7,442,785 B2 10/2008 Chappelletal. ........... .. 536/236 7,622,614 B2 11/2009 Julien etal. ..... .. .. 568/327 . - . 8,106,260 B2 1/2012 Chappelletal. .. .. 800/298 (75) Inventors ioslelgllghlai’pené?exmiwl?’ 8,192,950 B2 6/2012 Chappelletal. .. 435/41 ye - as 0“, ester 6 a ( ) 2003/0166255 A1 9/2003 Chappell .. 435/2523 2004/ 0078840 A1 4/ 2004 Chappell et . .. .. 800/278 (73) Assignee: University of Kentucky Research 2006/0218661 A1 9/2006 Chappellet a1. .. .. 800/278 Foundation, Lexington, KY (Us) 2007/0231861 A1 10/2007 Millis et a1. 435/69.1 2007/0238157 A1 10/2007 Millis et a1. 435/166 * - . - - - - 2007/0238159 A1 10/2007 Millis et a1. 435/25233 ( ) Nonce' SUbJeCt.t° any (3531mm? the garmgftgl; 2007/0238160 A1 10/2007 Millis et a1. 435/25233 Patent 15 men 6 or a Juste un er 2007/0254354 A1 11/2007 Millis et a1. 435/25233 U.S.C. 154(b) by 0 days. 2008/0178354 A1 7/2008 Chappelletal. 800/298 2008/0233622 A1 9/2008 Julien et a1. .. 435/148 (21) Appl.No.: 13/199,349 2010/0035329 A1 2/2010 Millisetal. 435/2542 2010/0120110 A1 5/2010 Chappell .. 435/166 - . 2010/0151519 A1 6/2010 Julien et a1. 435/69.1 (22) Flled' Aug' 26’ 2011 2010/0151555 A1 6/2010 Julien et a1. .. 435/193 _ _ _ 2010/0216186 A1 8/2010 Chappelletal. .. 435/69.1 (65) Prlor Publlcatlon Data 2011/0081703 A1 4/2011 Chappelletal. ............ .. 435/193 US 2011/0318797 A1 Dec. 29, 2011 FOREIGN PATENT DOCUMENTS JP 2000-511404 9/2000 Related US. Application Data WO 96/36697 11/1996 _ _ _ _ W0 97/38571 10/1997 (63) Contlnuatlon of apphcatlon No. 12/ 182,000, ?led on WO 97/33703 10/1997 Jul. 29, 2008, now Pat. No. 8,263,362, which is a W0 WO 97/37664 10/1997 continuationMar. 8, 2002, ofnow application Pat. No. 7,405,057.No. 10/097,559, ?led on WO 2010/019696 20010 (60) Provisional application No. 60/274,421, ?led on Mar. OTHER PUBLICATIONS 9, 2001, provisional application No. 60/275,597, ?led on Mar. 13’ 2001. Akiyoshi-Shibata et a1., “Further oxidation of hydroxycalcidiol by calcidiol 24 -hydroxylase. A study with the mature enzyme expressed in Escherichia coli,” Eur. J. Biochem. 224:335-343 (1994). (51) Int“ Cl“ An et al., “Organ-speci?c and developmental regulation of the C1 2N 1/00 (2006-01) nopaline synthase promoter in transgenic tobacco plants,” Plant C12N1/06 (2006.01) Physiol. 88:547-552 (1988). 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Back et al., “Cloning and bacterial expression of sesquiterpene U'S' PATENT DOCUMENTS cyclase, a key branch point enzyme for the synthesis of 5,589,619 A 12/1996 Chappell et a1. ............ .. 800/205 sesquiterpenoid phytoalexin capsidiol in UV-challenged leaves of 5,672,487 A 9/1997 SChweden et al~ ~~~~~~~~~ ~~ 435/69~1 Capsicum annuum,” Plant Cell. Physiol. 39:899-904 (1998). 5,741,674 A 4/1998 Schweden et al. ......... .. 435/69.1 5,766,911 A 6/1998 Koike et a1. 435/193 (Continued) 5,824,774 A 10/1998 Chappell et al. 530/350 _ _ _ _ 5,981,843 A 11/1999 Chappell et a1. 800/301 Prlmary Exam/"er * Medlna A Ibrahlm 2 gloteaunet ?l~l ~~ 5433672233? (74) Attorney, Agent, orFirm * McKenna Long &Aldridge , , appe et a . .. ~ - 6,100,451 A 8/2000 Chappell et a1. 800/298 LLP’ Stephame seldman 6,117,649 A 9/2000 Bellamine et al. .. 435/25 (57) ABSTRACT 2321323 3} 32221 285:: 2:1; ;; ;;; 83/92 The feamFes cytochrome 6,368,837 B1 4/2002 Gatenby et a1, , u 435/146 t1des and nuclelc a01d molecules, as well as expresslon vec 6,468,772 B1 10/2002 Chappell et a1. 435/183 tors and transgenic plants containing these molecules. In 6,495,354 B2 12/2002 Chappell et a1. 435/183 addition, the invention features uses of such molecules in 6,531,3036,559,297 B1B2 3/20035/2003 MillisChappell et a1.et al. 5433655135? methods of increasing the level of resistance against a disease 6,569,656 B2 5/2003 Chappell et al. 435/18'3 caused by a plant pathogen in a transgenic plant, in methods 6,645,762 B2 11/2003 Chappell et a1. 43 5 /3 2 5 for producing altered compounds, for example, hydroxylated 6,689,593 B2 2/2004 Millis et al. 435/155 compounds, and in methods of producing isoprenoid com 6,890,752 B2 5/2005 Chappell et a1. 435/325 pounds_ 7,186,891 B1 3/2007 Chappellet al. .. 800/298 7,405,057 B2 7/2008 Chappell et al. .......... 435/69.1 16 Claims, 11 Drawing Sheets US 8,445,231 B2 Page 2 OTHER PUBLICATIONS Arabidopsis thaliana (Accession No. X921117),” (Plant Gene Reg Beckman et al., “Human 25-hydroxyvitamin D3-24-hydroxylase, a ister PGR 96-037) Plant Physiol. 111:652 (1996). multicatalytic enzyme,” Biochem. 35:8465-8472 (1996). 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