Does batrachotoxin autoresistance co-evolve with toxicity in Phyllobates poison-dart frogs? Supplementary material Supplementary Tables Table S1. Specimen information and accession numbers of the sequences used in ancestral sequence reconstructions. For sequences obtained from publicly available genomes or transcriptomes where sequences do not have individual accession numbers (i.e. Nanorana parkeri, Rhinella marina, Rana pipiens) we provide the name of the contig or gene annotation. Species Voucher Locality DI DII DIII DIV Source Observations Allobates femoralis- Lumbaquí, Ecuador KT989177 - - KT989148 Tarvin et al. 2016 Allobates talamancae - Tundaloma, Ecuador KT989178 - - KT989149 Tarvin et al. 2016 Allobates zaparo- Río Pastaza, Ecuador KT989179 - - KT989150 Tarvin et al. 2016 Ameerega bilinguis- Venecia, Ecuador KT989180 KT989205 KT989220 KT989151 Tarvin et al. 2016 Ameerega hahneli- Canelos, Ecuador KT989181 - - KT989152 Tarvin et al. 2016 Ameerega parvula- Gualaquiza, Ecuador KT989182 KT989206 - KT989153 Tarvin et al. 2016 Panguana Biological Ameerega petersi GECOH1407 Station, Puerto Inca, Perú TBA - - TBA This Study Panguana Biological Ameerega picta GECOH1406 Station, Puerto Inca, Perú TBA - - TBA This Study Leticia, Amazonas, Ameerega trivittata TNHC-FS466 Colombia TBA - - TBA This Study La Celia, Risaralda, Andinobates bombetes GECOH1495 Colombia TBA - - TBA This Study Yotoco, Valle del Cauca, Andinobates bombetes GECOH297 Colombia TBA - - TBA This Study Andinobates fulguritus GECOH1314 Lloró, Chocó, Colombia TBA - - TBA This Study Cantón de San Pablo, Andinobates fulguritus GECOH1615 Chocó, Colombia TBA - - TBA This Study Fort Sherman, Colón, Colostethus panamansis TNHC-FS4889 Panama KT989193 - - KT989164 Tarvin et al. 2016 Dendrobates auratus ANW12085 - LT984935 - - - Yuan & Wang 2018 Dendrobates auratus ANW12086 - LT984936 - - - Yuan & Wang 2018 Dendrobates auratus ANW12087 - LT984939 - - - Yuan & Wang 2018 Dendrobates auratus ANW12088 - LT984937 - - - Yuan & Wang 2018 Dendrobates auratus ANW12164 - LT984938 - - - Yuan & Wang 2018 Dendrobates auratus ANW12221 - LT984933 - - - Yuan & Wang 2018 Dendrobates auratus ANW12222 - LT984932 - - - Yuan & Wang 2018 Lago Bayano, Panama, Dendrobates auratus GECOH1824 Panama TBA - - TBA This Study Dendrobates auratus GECOH1844 Veraguas, Panama TBA - - TBA This Study Dendrobates auratus IW163 - LT984940 - - - Yuan & Wang 2018 Dendrobates auratus IW175 - LT984934 - - - Yuan & Wang 2018 Dendrobates auratus IW177 - LT984926 - - - Yuan & Wang 2018 Dendrobates auratus IW931 - LT984927 - - - Yuan & Wang 2018 Dendrobates auratus IW933 - LT984928 - - - Yuan & Wang 2018 Dendrobates auratus IW934 - LT984929 - - - Yuan & Wang 2018 Dendrobates auratus IW936 - LT984930 - - - Yuan & Wang 2018 Dendrobates auratus IW937 - LT984931 - - - Yuan & Wang 2018 Captive bred, Understory Dendrobates tinctorius- Enterprises KT989189 KT989210 KT989224 KT989160 Tarvin et al. 2016 Dendrobates truncatus GECOH1589 Nuquí, Chocó, Colombia TBA - - TBA This Study Dendrobates truncatus GECOH2035 Lérida, Tolima, Colombia TBA - - TBA This Study Epipedobates anthonyi - El Progreso, Ecuador KT989195 - KT989166 Tarvin et al. 2016 Epipedobates boulengeri - Arasha, Ecuador KT989196 KT989212 KT989227 KT989167 Tarvin et al. 2016 Epipedobates boulengeri Buenaventura, Valle del north GECOH594 Cauca, Colombia TBA - - TBA This Study Epipedobates boulengeri Buenaventura, Valle del north GECOH640 Cauca, Colombia TBA - - TBA This Study Epipedobates boulengeri Guapi, Cauca, Colombia north GECOH691 (mainland) TBA - - TBA This Study Epipedobates darwinwallacei- Mindo, Ecuador KT989197 - - KT989168 Tarvin et al. 2016 Epipedobates machalilla - Manta Real, Ecuador KT989198 - - KT989169 Tarvin et al. 2016 Epipedobates tricolor - Chazo Juan, Ecuador KT989199 KT989213 KT989229 KT989170 Tarvin et al. 2016 Espadarana callistomma - Alto Tambo, Ecuador KT989175 KT989203 KT989217 KT989144 Tarvin et al. 2016 Gastrotheca litonedis- Ñamarín, Ecuador KT989173 KT989202 KT989216 KT989143 Tarvin et al. 2016 Hyloxalus italoi- Canelos, Ecuador KT989184 KT989207 KT989221 KT989155 Tarvin et al. 2016 Hyloxalus nexipus- Río Negro, Ecuador KT989185 KT989208 KT989222 KT989156 Tarvin et al. 2016 Hypsiboas picturatus El Placer, Ecuador - KT989204 KT989218 KT989146 Tarvin et al. 2016 Incilius nebulifer TNHC-FS6877 Austin, Texas, USA KT989172 KT989201 KT989215 KT989142 Tarvin et al. 2016 Méndez, Morona Lithodytes lineatus QCAZ16621 Santiago, Ecuador KT989174 - - KT989145 Tarvin et al. 2016 Captive bred, Understory Mantella aurantiaca- Enterprises KT989171 KT989200 KT989214 KT989141 Tarvin et al. 2016 Obtained from Npar_R017212 Nanorana parkeri- Sun et al. 2015 genome Oophaga granulifera IW1005 Rio Baru LT984951 - - - Yuan & Wang 2018 Oophaga granulifera IW1006 Rio Baru LT984950 - - - Yuan & Wang 2018 Oophaga granulifera IW1007 Rio Baru LT984949 - - - Yuan & Wang 2018 Oophaga granulifera IW1008 Rio Baru LT984948 - - - Yuan & Wang 2018 Oophaga granulifera IW1010 - LT984947 - - - Yuan & Wang 2018 Oophaga granulifera IW1012 Drake Bay LT984946 - - - Yuan & Wang 2018 Oophaga granulifera IW1013 Drake Bay LT984945 - - - Yuan & Wang 2018 Oophaga granulifera IW1014 Drake Bay LT984944 - - - Yuan & Wang 2018 Oophaga granulifera IW926 Fila Chonta LT984943 - - - Yuan & Wang 2018 Oophaga granulifera IW928 Fila Chonta LT984942 - - - Yuan & Wang 2018 Oophaga granulifera IW939 Rio Saverge LT984941 - - - Yuan & Wang 2018 Buenaventura, Valle del Oophaga histrionica GECOH1002 Cauca, Colombia TBA - - TBA This Study Buenaventura, Valle del Oophaga histrionica GECOH1008 Cauca, Colombia TBA - - TBA This Study Oophaga pumilio GECOH2007 Kilambé, Nicaragua TBA - - TBA This Study Oophaga pumilio IW001 - LT984967 - - - Yuan & Wang 2018 Oophaga pumilio IW002 - LT984968 - - - Yuan & Wang 2018 Oophaga pumilio IW003 - LT984969 - - - Yuan & Wang 2018 Oophaga pumilio IW004 - LT984970 - - - Yuan & Wang 2018 Oophaga pumilio IW016 - LT984955 - - - Yuan & Wang 2018 Oophaga pumilio IW018 - LT984956 - - - Yuan & Wang 2018 Oophaga pumilio IW019 - LT984971 - - - Yuan & Wang 2018 Oophaga pumilio IW020 - LT984957 - - - Yuan & Wang 2018 Oophaga pumilio IW021 - LT984958 - - - Yuan & Wang 2018 Oophaga pumilio IW047 - LT984965 - - - Yuan & Wang 2018 Oophaga pumilio IW048 - LT984974 - - - Yuan & Wang 2018 Oophaga pumilio IW050 - LT984972 - - - Yuan & Wang 2018 Oophaga pumilio IW052 - LT984973 - - - Yuan & Wang 2018 Oophaga pumilio IW053 - LT984959 - - - Yuan & Wang 2018 Oophaga pumilio IW055 - LT984966 - - - Yuan & Wang 2018 Oophaga pumilio IW092 - LT984960 - - - Yuan & Wang 2018 Oophaga pumilio IW093 - LT984961 - - - Yuan & Wang 2018 Oophaga pumilio IW094 - LT984975 - - - Yuan & Wang 2018 Oophaga pumilio IW095 - LT984976 - - - Yuan & Wang 2018 Oophaga pumilio IW096 - LT984962 - - - Yuan & Wang 2018 Oophaga pumilio IW142 - LT984977 - - - Yuan & Wang 2018 Oophaga pumilio IW143 - LT984978 - - - Yuan & Wang 2018 Oophaga pumilio IW169 - LT984963 - - - Yuan & Wang 2018 Oophaga pumilio IW171 - LT984952 - - - Yuan & Wang 2018 Oophaga pumilio IW180 - LT984954 - - - Yuan & Wang 2018 Oophaga pumilio IW181 - LT984964 - - - Yuan & Wang 2018 Oophaga pumilio IW182 - LT984953 - - - Yuan & Wang 2018 Oophaga pumilio IW189 - LT984979 - - - Yuan & Wang 2018 Oophaga pumilio IW193 - LT984986 - - - Yuan & Wang 2018 Oophaga pumilio IW194 - LT984980 - - - Yuan & Wang 2018 Oophaga pumilio IW196 - LT984981 - - - Yuan & Wang 2018 Oophaga pumilio IW197 - LT984982 - - - Yuan & Wang 2018 Oophaga pumilio IW199 - LT984983 - - - Yuan & Wang 2018 Oophaga pumilio IW200 - LT984984 - - - Yuan & Wang 2018 Oophaga pumilio IW201 - LT984985 - - - Yuan & Wang 2018 Phyllobates aurotaenia north GECOH1145 Nuquí, Chocó, Colombia TBA - - TBA This Study Phyllobates aurotaenia north GECOH1155 Nuquí, Chocó, Colombia TBA - - TBA This Study Phyllobates aurotaenia north GECOH1307 Lloró, Chocó, Colombia TBA - - TBA This Study Phyllobates aurotaenia north GECOH1484 Tadó, Chocó, Colombia TBA - - TBA This Study Phyllobates aurotaenia north GECOH705 Tadó, Chocó, Colombia TBA - - TBA This Study Phyllobates aurotaenia north GECOH706 Tadó, Chocó, Colombia TBA - - TBA This Study Phyllobates aurotaenia Buenaventura, Valle del south GECOH1170 Cauca, Colombia TBA - - TBA This Study Phyllobates aurotaenia Buenaventura, Valle del south GECOH1190 Cauca, Colombia TBA - - TBA This Study Phyllobates aurotaenia Buenaventura, Valle del south GECOH1194 Cauca, Colombia TBA - - TBA This Study Captive bred, Parents from Pueblo Rico, TBA Obtained from Phyllobates bicolor- Risaralda, Colombia This Study transcriptome San José del Palmar, Phyllobates bicolor GECOH1039 Chocó, Colombia TBA - - TBA This Study Pueblo Rico, Risaralda, Phyllobates bicolor GECOH1174 Colombia TBA - - TBA This Study Pueblo Rico, Risaralda, Phyllobates bicolor GECOH1176 Colombia TBA - - TBA This Study Pueblo Rico, Risaralda, Phyllobates bicolor GECOH1485 Colombia TBA - - TBA This Study Pueblo Rico, Risaralda, Phyllobates bicolor GECOH523 Colombia TBA - - TBA This Study Isla Colón, Panamá, Phyllobates lugubris GECOH1865 Panamá TBA - - TBA This Study Isla Veraguas, Panamá, Phyllobates lugubris GECOH1866 Panamá TBA - - TBA This Study Puerto Viejo de talamanca, Limón, Costa Phyllobates lugubris GECOH963 Rica TBA - - TBA This Study Puerto Viejo de talamanca, Limón, Costa Phyllobates lugubris GECOH964 Rica TBA - - TBA This Study Puerto Viejo de talamanca, Limón, Costa Phyllobates lugubris GECOH966 Rica TBA - - TBA This Study Captive bred, Black Phyllobates terribilis- Jungle Terrarium Supply KT989191 KT989211 -