Anti-MASTL monoclonal antibody, clone SJQMZ 85D (DCABH-4990) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Product Overview Mouse monoclonal to MASTL Antigen Description Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at Ser-62 and Ser-67, respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. Immunogen Recombinant full length protein, corresponding to amino acids 1-879 of Human MASTL with a 26kDa proprietary tag. Isotype IgG1 Source/Host Mouse Species Reactivity Mouse, Human Clone SJQMZ 85D Purity Protein G purified Conjugate Unconjugated Applications WB, IHC-P Positive Control Human and Mouse MASTL-transfected HEK cell lysates; Raji, Ramos, Dana and MT2 cell lysates; 293T and HeLa transfected cell lysates; Human tonsil, testicle, bone marrow, Hodgkin and Burkitt lymphoma tissues. Format Liquid Size 100 μg Buffer pH: 7.40; Constituents: PBS, 1% BSA 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Preservative None Storage Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. GENE INFORMATION Gene Name MASTL microtubule associated serine/threonine kinase-like [ Homo sapiens ] Official Symbol MASTL Synonyms MASTL; microtubule associated serine/threonine kinase-like; serine/threonine-protein kinase greatwall; FLJ14813; THC2; greatwall protein kinase; microtubule-associated serine/threonine- protein kinase-like; GW; GWL; hGWL; MAST-L; GREATWALL; RP11-85G18.2; Entrez Gene ID 84930 Protein Refseq NP_001165774 UniProt ID Q96GX5 Chromosome Location 10p12.1 Function ATP binding; kinase activity; nucleotide binding; protein phosphatase 2A binding; protein serine/threonine kinase activity; 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.