Human SLC14A1 Antibody Monoclonal Mouse IgG2B Clone # 888418 Catalog Number: MAB8238 DESCRIPTION Species Reactivity Human Specificity Detects human SLC14A1 in direct ELISAs. Source Monoclonal Mouse IgG2B Clone # 888418 Purification Protein A or G purified from hybridoma culture supernatant Immunogen NS0 mouse myeloma cell line transfected with human SLC14A1 Accession # Q13336 Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (­SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS. APPLICATIONS Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website. Recommended Sample Concentration Flow Cytometry 2.5 µg/106 cells See Below CyTOF­ready Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. DATA Flow Cytometry Flow Cytometry Detection of SLC14A1 in Human Red Detection of SLC14A1 in HEK293 Human Cell Line Blood Cells by Flow Cytometry. Human red Transfected with Human SLC14A1 and eGFP by Flow blood cells were stained with Mouse Anti­ Cytometry. HEK293 human embryonic kidney cell line transfected Human SLC14A1 Monoclonal Antibody with human SLC14A1 and eGFP was stained with either (A) Mouse (Catalog # MAB8238, filled histogram) or Anti­Human SLC14A1 Monoclonal Antibody (Catalog # MAB8238) isotype control antibody (Catalog # MAB0041, or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # open histogram), followed by MAB0041) followed by Allophycocyanin­conjugated Anti­Mouse IgG Allophycocyanin­conjugated Anti­Mouse IgG Secondary Antibody (Catalog # F0101B). Secondary Antibody (Catalog # F0101B). PREPARATION AND STORAGE Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS. Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (­SP) is shipped with polar packs. Upon receipt, store it immediately at ­20 to ­70 °C Stability & Storage Use a manual defrost freezer and avoid repeated freeze­thaw cycles. l 12 months from date of receipt, ­20 to ­70 °C as supplied. l 1 month, 2 to 8 °C under sterile conditions after reconstitution. l 6 months, ­20 to ­70 °C under sterile conditions after reconstitution. BACKGROUND Urea transporter 1, designated Solute Carrier family 14 member 1 (SLC14A1) is a 43­54 kDa multipass urea transporter mainly expressed in erythrocytes, but also found in kidney, mesenchymal stem cells, and some carcinomas. It contains an epitope identified as the Kidd (JK) blood group antigen. The antigenic forms differ at only one amino acid (aa); Asp280 represents Jk(a) and Asn280 represents Jk(b). SLC14A1 Isoform 2 (445 aa) contains an extended N­terminus as compared to Isoform 1 (389 aa). Human SLC14A1 shares 83% and 81% aa sequence identity with mouse and rat SLC14A1, respectively. Rev. 2/7/2018 Page 1 of 1 .