Program & Abstracts

Program & Abstracts

SOUTHWESTERN ASSOCIATION OF PARASITOLOGISTS 54th Annual Meeting Program & Abstracts April 23, 2021 Held Virtually via Zoom Affiliate, American Society of Parasitologists SOUTHWESTERN ASSOCIATION OF PARASITOLOGISTS OFFICERS President Heather Stigge Department of Biology College of Saint Mary Omaha, NE 68106 President-Elect (Program Officer) Joanna J. Cielocha Department of Biology Rockhurst University Kansas City, MO 64110 Immediate Past President Kristin Herrmann Department of Biological Sciences Tarleton State University Stephenville, TX 76049 Secretary-Treasurer Autumn Smith-Herron Office of Research and Sponsored Programs Sam Houston State University Huntsville, TX 77341 Representative to ASP Council Kirsten Jensen Department of Ecology and Evolutionary Biology University of Kansas Lawrence, KS 66045 AGENDA Friday, April 23rd, 2021 8:00–9:00 am President’s Welcome & SWAP Business Meeting 9:00–9:15 am Break 9:15–10:30 am Session A Oral Presentations (#1–5) 10:30–10:45 am Break 10:45–12:00 pm Session B Oral Presentations (#6–10) 12:00–1:00 pm Lunch Break 1:00–2:15 pm Session C Oral Presentations (#11–15) 2:15–2:30 pm Break 2:30–3:45 pm Session D Oral Presentations (#16–20) 3:45–4:00 pm Break 4:00–4:45 pm Session F Oral Presentations (#21–23) 4:45–5:00 pm Closing Remarks & Adjournment *Abstracts with a “UG” are Undergraduate student papers in the competition; those with a “G” are Graduate student papers in the competition. Unless noted, the speaker is the first author listed. 1 8:00–9:00 am President’s Welcome & SWAP Business Meeting President HEATHER STIGGE 9:00–9:15 am Break 9:15–10:30 am Session A: Oral Presentations 1–5 Chairpersons RUSSELL LEE, Creighton University ANNA SNIEZEK, Creighton University 9:15 am (G) 1. Thermal Preferenda of the Red-rimmed Melania (Melanoides tuberculata): Variation Among Clonal Lineages and Infection. Matthew Donelon and David Huffman. Biology Department, Texas State University, San Marcos, Texas. The invasive thiarid snail Melanoides tuberculata serves as host for more trematodes than any other snail. Local populations in central Texas host two invasive heterophyid trematodes which are impacting several fish species of concern. The reported vulnerability of the snail to winter thermal minima of Texas rivers was earlier expected to restrict its distribution to thermally stable spring waters like Comal Springs in New Braunfels. However, in 2009, M. tuberculata was found thriving in the connecting Guadalupe River many km from spring influence, despite recorded temperatures in those reaches remaining well below the published lethal thermal minimum of the snail for weeks. The invasive populations of the parthenogenetic snail are not composed of a single traditional species, but of several genetically distinct clonal lineages which were likely collected from thermally different environments in the snail’s native range. Unfortunately, all previous researchers reporting on the thermal ecology of the snail ignored the clonal identities of experimental subjects. I searched the thermal biology literature hoping to find a device suitable for exploring the thermal preferenda of local clones of the snail. Unable to find a suitable device, I designed and constructed a water-filled thermal-gradient trough based on countercurrent heat-exchange theory and driven by a computer-controlled Peltier heat source at one end and a Peltier heat sink at the other end. The device is designed to produce a stable, linear thermal gradient ranging ±15 C from the 23 C waters of local springs. I will collect representative clones from Central Texas waters, and explore their thermal preferenda under varying conditions. I anticipate that the clones will differ in thermal preferenda, and that other factors (water quality, parasitized/uninfected, fasted/satiated, light/dark etc.) will alter some of the thermal responses. 2 9:30 am (G) 2. Mediterranean parasites in North America: Coccidia from introduced populations of the Mediterranean house gecko, Hemidactylus turcicus and other species of Hemidactylus. Allison Bryant1, Matthew Bolek1, and Gabriel Langford2. 1Department of Integrative Biology, Oklahoma State University, Stillwater, Oklahoma and 2Department of Biology, Florida Southern College, Lakeland, Florida. Hemidactylus turcicus, is native to the Mediterranean region of Africa, Asia, and Europe. However, it has been widely introduced across the world including the United States, were it was first reported in Florida in 1910 and have since colonized the southern United States. Beginning in the 1960s, the first records of H. turcicus from Oklahoma were due to intentional releases on university campuses, including Oklahoma State University, and currently H. turcicus is found in 55 of 77 counties in the state. Although a number of studies have documented parasites of H. turcicus throughout its introduced range, little information exists on the coccidian parasites of this gecko. We sampled geckos from the OSU campus and examined their fecal samples for coccidian oocysts. Twenty percent of geckos shed ellipsoid tetrasporocystid oocysts with a smooth bi-layered wall. Oocysts, contained a polar granule, but a micropyle and residuum were absent. Sporocysts were ellipsoid and without stiedal or substiedal bodies. Sporocysts contained a residuum composed of numerous granules in a spherical mass and 2 sporozoites. These oocysts were most similar to the description of Eimeria lineri (Acroeimeria lineri) previously reported from H. turcicus in the United States, Egypt, Israel and Turkey and from H. mabouia, from South Africa. Additionally, 20% of geckos shed ellipsoid polysporocystid oocysts with a smooth bi-layered wall. Numerous granules were present within oocysts, but a micropyle was absent. The number of sporocysts per oocyst ranged from 8 to 12. Sporocysts were subspherical and contained a residuum composed of scattered granules. These oocysts were most similar to species of Adelina which infect invertebrate hosts and should be considered pseudoparasites of geckos. This is the first report of Acroeimeria lineri from Oklahoma. However, a recent study on coccidia of H. turcicus from Egypt suggested that A. lineri may represent a complex of cryptic species. As a result, we are expanding our survey and examining Mediterranean house geckos and two other introduced species of Hemidactylus, from Florida for coccidian infections. To get a better understanding of the diversity of coccidian parasites in species of Hemidactylus, we will utilize DIC microscopy techniques for oocyst morphology, histological techniques for coccidian development in geckos, and molecular techniques for barcoding coccidians. 3 9:45 am (UG) 3. A survey of gregarine species parasitizing the differential grasshopper, Melanoplus differentialis, in an urban environment in eastern Kansas, U.S.A. Jordan A. Xufuris and J. J. Cielocha. Department of Biology, Rockhurst University, Kansas City, Missouri, U.S.A. Gregarines are a diverse group of protozoan parasites that parasitize marine, freshwater and terrestrial invertebrates. Gregarines of insects are especially diverse. The insect order Orthoptera includes grasshoppers, katydids, locusts and crickets. Several past studies have focused on gregarines of orthopterans, but few are from the Americas. The differential grasshopper, Melanoplus differentialis, has been reported to host a gregarine in the genus Amoebogregarina in Illinois, Nebraska, and Ohio throughout the 1900s. Twenty-eight individuals of M. differentialis were collected by hand from a fence line along a public walking trail in Overland Park, Kansas, U.S.A. in the fall of 2019. Grasshoppers were stored in 1- gallon plastic containers with the host plant and dissected 24 to 48 hours after collection. Alimentary canals were removed, dissected, and observed for gregarine infection. Specimens were fixed, stained, and mounted on glass slides for observation under a compound microscope. Two gregarine species were found parasitizing M. differentialis in this study. One species is consistent with that previously described from this host, Amoebogregarina c.f. nigra. The second is presumably a new species of Quadruspinospora. It differs from the other known members of this genus by a number of morphological features such as total length and maximum width, but also in its developmental sequence. Here we report the differences and diversity of gregarines in the genus Quadruspinospora and discuss the taxonomic history and present status of this genus. Future work will focus on obtaining molecular sequence data to better determine the phylogenetic placement of these taxa among the Eugregarinorida. 4 10:00 am (UG) 4. Prevalence of Toxoplasma gondii among cat populations in Southeast Nebraska. Tyra Mollhoff, Noah Kasbohm, and Gul Ahmad. Department of Natural Sciences, School of Arts & Sciences, Peru State College, Peru NE 68421. Domestic cats (Felis catus) are beloved companions and important animals that many humans live close to or encounter every day. However, cats are also profoundly important carriers for many zoonotic diseases. One of the unicellular eukaryotic parasites which cause a deadly disease in human and are transmitted via cats is toxoplasmosis. The causative agent of this debilitating disease is the protozoan parasite Toxoplasma gondii. Currently some 16% of the United States population is seropositive for toxoplasmosis while up to 2% of the mentally retarded children are reportedly born to mothers who were infected with Toxoplasma gondii during the pregnancy.

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