US010709151B2 United States Patent (10 ) Patent No.: US 10,709,151 B2 Ulmasov et al. (45 ) Date of Patent : Jul. 14 , 2020 ( 54 ) LOW FIBER PENNYCRESS MEAL AND WO 20090148336 A1 12/2009 METHODS OF MAKING WO 2010002984 A1 1/2010 WO 2016055838 Al 3/2019 (71 ) Applicants : COVERCRESS INC. , St. Louis , MO (US ) ; Board of Trustees of Illinois OTHER PUBLICATIONS State University , Normal , IL (US ) ; Alhotan et al , 2017 , Poultry Science , 96 :2281-2293 . * Regents of the University of Selling et al , 2014 , Industrial Crops and Products , 41 :113-119 . * Minnesota , Minneapolis , MN (US ) Vaughn et al , 2005 , Journal of Chemical Ecology , 31 : 167-177 . * Badani et al, 2006 , Genome, 49 : 1499-1509 . * (72 ) Inventors : Tim Ulmasov , Chesterfield , MO (US ) ; Tsogtbaatar et al , 2015 , Journal of Experimental Biology, 66 : 4267 4277. * Gary Hartnell, St. Peters, MO (US ) ; Li et al , 2015 , Journal of Agriculture and Food Chemistry , 63: 9590 John C. 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(52 ) U.S. CI. Lian et al ., “ Silencing of BnTTi Family Genes Affects Seed CPC Flavonoid Biosynthesis and Alters Seed Fatty Acid Composition in A23K 10/30 ( 2016.05 ) ; A23K 20/158 Brassica napus” , Plant Science , 2017 , pp . 32-47 , vol . 254 . (2016.05 ) National Institute of Food and Agriculture , “ Advancing Field Pennycress (58 ) Field of Classification Search as a New Oilseed Biodiesel Feedstock that Does Not Require New None Land Commitments Annual Report ” ,Accession No. 1004021, Retrieved See application file for complete search history. from https://portal.nifa.usda.gov/web/crisprojectpages/1004021 on Nov. 26 , 2018 , 10 pages . ( 56 ) References Cited (Continued ) U.S. PATENT DOCUMENTS Primary Examiner Jason Deveau Rosen 4,933,166 A 6/1990 Shen et al. (74 ) Attorney, Agent, or Firm Thompson Coburn LLP ; 7,960,612 B2 * 6/2011 Zhang CO7K 14/415 Charles P. Romano 800/298 2004/0045049 Al 3/2004 Zhang et al. (57 ) ABSTRACT 2006/0150283 A1 7/2006 Alexandrov et al . 2009/0138981 A1 5/2009 Repetti et al . Pennycress seed , seed lots , and seed meal having reduced 2016/0138034 Al 5/2016 Hancock et al. fiber content and improved suitability for use in producing animal feed are provided . FOREIGN PATENT DOCUMENTS EP 0312763 A1 4/1989 24 Claims, 6 Drawing Sheets WO 2006010096 A2 1/2006 Specification includes a Sequence Listing . US 10,709,151 B2 Page 2 ( 56 ) References Cited United States Department ofAgriculture , “ Advancing Field Pennycress as a New Oilseed Biodiesel Feedstock that does not Require New Land Commitments Annual Progress Report ” , Accession No. 1004021 , Date Submitted to NIFA Jan. 9 , 2018 , 7 pages . 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(H2SO4 ) in Animal Feed ” , © 2000 AOAC International , down Slominski et al . , “ Nutritive Value for Broilers of Meals Derived loaded from http://www.cankaowuzhi.com/document/guowai/ from Newly Developed Varieties of Yellow - Seeded Canola ” , Ani AOAC973_18.pdf Mar. 21, 2020 . mal Feed Science and Technology, 1999 , pp . 249-262 , vol . 78 . Herbicide safener definition downloaded from the National Agri United States Department ofAgriculture , “ Advancing Field Pennycress cultural Library of the US Department of Agriculture website as a New Oilseed Biodiesel Feedstock that does not Require New https://agclass.nal.usda.gov/glossary.shtml on Mar. 25 , 2020 . Land Commitments Annual Progress Report ” , Accession No. 1004021, Date Submitted to NIFA Dec. 6 , 2016 , 9 pages . * cited by examiner U.S. Patent Jul. 14 , 2020 Sheet 1 of 6 US 10,709,151 B2 A. B. C. FIGURE 1A , B , C U.S. Patent Jul. 14 , 2020 Sheet 2 of 6 US 10,709,151 B2 A. inito GANA scaffold » 1710 17 MAS promoter AQU6-1Atvopromoter :29 promoter 7000 3,7,000 I81Kank-NO eade PARV8 ( S $ 51.7710 ) DVS1Staa 191335SPPDKwe promoter C. B. FIGURE 2A , B U.S. Patent Jul. 14 , 2020 Sheet 3 of 6 US 10,709,151 B2 14,000 PVSIoriv bom site from PBR322 Se 20001 Aørt ( 2237 ) bom Rep2 AtU6-1 RepOrigin_1 Aart ( 2269 ) LaT- DNA repeat promoter segons 12,000 OVS1Stan pea3Aterm PARV187_FnCpfi Base Vector 14,845 OS SINQuseidohuni 4000 NOSterminator RBT-ONArepeat NE 000'OTI EGFP CMVPromoter 3xFLAG 16000 Set FIGURE 3 U.S. Patent Jul. 14 , 2020 Sheet 4 of 6 US 10,709,151 B2 bom site from? POR322 mal PVS1oriv pop ? Repz 2000 12,000 Rep.Origin LBP-ONA T-repeatDNA LB AU6-1 promoter PVS1Staa PARV191.Smcsmi Base Vector 12,137 bp HucieoplasminMLS RB7-DNA repeat 14000 10,000 ONOSterminator EGFPCMV Prornoter 3xFLAG 16000 00084 PSS TODOC FIGURE 4 U.S. Patent Jul. 14 , 2020 Sheet 5 of 6 US 10,709,151 B2 A. 4.5 4. 4.4.4 ... -444 tikel 08176869.Tasarvence dna 3 . B. 772 17506 Thiesos srvente csap.S C.