R+D+I SERVICES OFFERED BY THE CBET RESEARCH GROUP: ASSESSMENT AND MONITORING OF ENVIRONMENTAL POLLUTION AND CHEMICAL TOXICITY TESTING www.ehu.es/GrupoBCTA MP Cajaraville*, I Marigómez, E Angulo, C Barbero, M Soto, T Serrano, I Cancio, A Orbea, M Ortiz-Zarragoitia, B Zaldibar, E Bilbao, U Izagirre, L Garmendia, V Asensio, A Rodríguez, P Ruiz, E Puy-Azurmendi, O Díaz de Cerio, A Katsumiti, U Vicario, M Múgica, A Irizar, A Jimeno, A de los Ríos, L Pérez Dept Zoology and Cell Biology, Lab Cell Biology and Histology, Fac. Science and Technology, UPV/EHU. * E-mail contact: [email protected], Web page: www.ehu.es/GrupoBCTA Since 1985 the group of “Cell Biology in Environmental Toxicology” of the University of the Basque Country develops research and teaching activities within the Environmental Toxicology field. The group is recognized since 2001 as a consolidated research group. The main field of expertise is in the development and application of early warning cell and molecular markers of pollution. The group has experience in the assessment and monitoring of biological effects of pollution (North Iberian Peninsula, Mediterranean Sea, North Sea), including accidental oil spills (Prestige) and industrial and urban wastes. The group promotes the Marine Station of Plentzia. We offer the following R+D+I services: -Assessment of environmental quality (marine, estuarine, and terrestrial environments). -Monitoring programmes of environmental pollution. -Toxicity testing (REACH legislation). -Determination of biological activity of chemicals. -Histopathological diagnosis of fish and shellfish diseases. -Scientific and technical advising. Areas: pollution, accidental spills, environmental toxicology, use of biomarkers, cell culture and in vitro systems, microscopy, molecular, cell and tissue organization of animals. -Scientific formation: Masters, postgraduate courses, specialization courses, PhD programme, laboratory training. -Estabulation and maintenance of experimental animals. Marine aquaria. Zebrafish facility. Facilities to run chemical exposures. -Processing of samples for histology, light and electron microscopy, cryotechniques. -Quantification of levels of specific proteins, enzyme activities, gene expression, immunolabelling, cell structure. ASSESSMENT & MONITORING OF ENVIRONMENTAL BIOLOGICAL ACTIVITY OF CHEMICALS & TOXICITY POLLUTION TESTING Assessment of polluted .- HistopathologIcal diagnosis soils (derelict mines…) BECPELAG 2002 WCM 2003, 2004, 2008 Eosinophilic foci Hepatocellular S adenoma ince Gonad histology 199 1 Laboratory exposure Hepatocarcinoma experiments Stages of liver cancer BEEP project 2001-04 progression in Marine biomonitoing in the Bay of Biological effects measurement of oil fish Biscay, Mediterranean and North extraction and refinery processes Seas Intersex fish gonad Microarrays & In vitro toxicity Genotoxicity gene expression POLLUTION SENTINEL SPECIES tests tests profiles .- Chemical toxicity tests (REACH) Since the early 80s, CBET has been investigating the biological effects of contaminants in both terrestrial and aquatic ecosystems with a common conceptual and methological approach (biomarkers + bioassays .- Development and application of chemical exposure and effect biomarkers in + bioanalytical chemistry) applied to target sentinel species that are representative of the corresponding experimental animals (fish, molluscs, earthworms) exposed to sublethal ecosystem in terms of health. concentrations of chemical compounds in laboratory conditions SCIENTIFIC & TECHNICAL ADVISING FORMATION: MASTERS, POSTGRADUATE COURSES THE CASE OF THE PRESTIGE OIL SPILL European Master of Science in Marine Environment and Resources 1 2 3 4 5 6 7 8 9 10 1. April-03 JOINT EUROPEAN POSTGRADUATE STUDIES S. Bartolomeu * * 2. July-03 Ons Cellular level: * * * QUALITY AWARD – Spanish MICINN: MDC (2008-2012) Cies 3. September-03 * ** AOXexp, AOXeff, LP, VvL MERMERMER Oia 4. April-04 Tissue level: * * * * Aguiño 5. July-04 Vv , CI * * * * * BAS IR Caldebarcos 6. October-04 * * * ** .- Scientific based Camelle 7. April-05 * * * * information for Segaño * * * * ** 8. July-05 http://www.ehu.es/MER Estaca administrations and * * * * * 9. October-05 Llanes * * ** ** 10. April-06 stakeholders on S. Vicente HSI * * * * * ** * Suances environmental pollution: * * * * * * Ingurumen Kutsadura eta Toxikologia Pedreña ICES, UNEP, Le Cedre Healthy * * * * * * ** Laredo * * Contaminacion y Toxicologia Ambientales C * * Low stress Muskiz * * * ** .- Development of environment Arrigunaga Environmental Contamination & Toxicology Medium stress * * * * pollution related legislation (soil Gorliz A * * * * * * High stress Bakio law…), contingency plans (oil POSTGRADUATE MASTER - UPV/EHU * * * ** Mundaka spills) T = Exposure to organic xenobiotics *** * * * * QUALITY AWARD – Spanish MICINN: MDC (2005-2012) * Mutriku (AOX exp) ** * * * Orio * ** * * * * .- Industrial waste disposal * http://www.ehu.es/CTA Ecosystem health indices Hondarribia * * * *** management FACILITIES FOR MICROSCOPY, AQUACULTURE & Examples of recent collaborations-contracts with enterprises: CHEMICAL EXPOSURES -PETRONOR S.A.: contract for biomonitoring of the waste discharge site (Punta Lucero, Bizkaia) of the petrol refinery (2000 up to now). -NORSK HYDRO and NIVA (Norway): contract for biomonitoring of the North Sea (Water Column monitoring 2003, 2004-2005 and 2008). -IHOBE S.L.: contract to make an inventory of affections in the Basque Coast after Prestige oil spill (2003-2004). -CIS S.L. (Galicia): contract for monitoring of the impact of the Prestige oil spill on the Cies and Zebrafish Ons islands (2004-2006). Biological experimentation -LE CEDRE (France): EU contract “Pollution monitoring and ecological impact assessment Electron facility following accidental oil and other chemical spills in marine waters” (2007). Microscopy -IRIS (Norway): EU project PRAGMA “A pragmatic and integrated approach for the evaluation of environmental impact of oil and chemicals spilled at sea: input to European guidelines”, 2006-2007. PLENTZIAKO ITSAS-ESTAZIOA (PIE) UPV-EHU -DEAD SEA LABORATORIES (Israel), INTRINSIQ (UK): EU project NANORETOX “The Experimental reactivity and toxicity of engineered nanoparticles: risks to the environment and human health” Biology and Biotechnology (2008-2012). Marine Station -AZTI Foundation (Sukarrieta), INBIOMED FOUNDATION (San Sebastian) and BIOBIDE S.L. (San Sebastian): work together in the Spanish technological platform DareNet to promote the use of zebrafish in research. Support the Spanish MSI project NANOCANCER “Determination of genotoxic and carcinogenic potential of metal nanoparticles using alternative methods in vitro and in vivo with zebrafish and invertebrates” (2010-2012). -FULCRUM (Leioa): Spanish Ministry of Environment project VERTITOX “Development of Marine Station of Plentzia to be inaugurated Aquarium facilities for chemical exposure experiments in UPV/EHU procedures for the integrated operative control of urban and industrial effluents into the coastal end of 2010 area” (2008-2010)..