GANAB Antibody Cat. No.: 15-233 GANAB Antibody Immunohistochemistry of paraffin-embedded Immunohistochemistry of paraffin-embedded mouse brain using rat brain using GANAB antibody (15-233) at GANAB antibody (15-233) at dilution of 1:150 (40x lens). dilution of 1:150 (40x lens). Immunofluorescence analysis of C6 cells using Immunofluorescence analysis of NIH-3T3 cells using GANAB GANAB antibody (15-233) at dilution of 1:100. antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear Blue: DAPI for nuclear staining. staining. September 29, 2021 1 https://www.prosci-inc.com/ganab-antibody-15-233.html Immunofluorescence analysis of U-2 OS cells Immunofluorescence analysis of C6 cells using GANAB antibody using GANAB antibody (15-233) at dilution of (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining. 1:100. Blue: DAPI for nuclear staining. Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of IMMUNOGEN: human GANAB (NP_001265123.1). TESTED APPLICATIONS: IF, IHC, WB WB: ,1:500 - 1:2000 APPLICATIONS: IHC: ,1:50 - 1:200 IF: ,1:50 - 1:200 POSITIVE CONTROL: 1) U-87MG 2) Raji 3) HepG2 4) HeLa 5) Mouse brain 6) Mouse liver PREDICTED MOLECULAR Observed: 112kDa WEIGHT: September 29, 2021 2 https://www.prosci-inc.com/ganab-antibody-15-233.html Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: GANAB GANAB, G2AN, Glucosidase, alpha, Glucosidase, alpha neutral AB, Neutral alpha- ALTERNATE NAMES: glucosidase AB, Alpha-glucosidase 2, Glucosidase II subunit alpha, GLUII, KIAA0088, Neutral AB GENE ID: 23193 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature BACKGROUND: glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 29, 2021 3 https://www.prosci-inc.com/ganab-antibody-15-233.html.