Supplemental Figure Legends Supplemental Figure 1. Expression of cytoplasmic immunoglobulin in D4 cell subsets. A. Phenotype of D4 cell subsets. B. Expression of cyIgM, cyIgA, and cyIgG in D4 cell subsets. Results are those of one experiment representative of three, SI means Staining Index. C. Expression of cyIgM, cyIgA, and cyIgG in D4 cell subsets. Results are mean SI obtained in three separate experiments. * The mean expression is significantly different from that in CD20high B cells. ** The mean expression is significantly different from that in D4 CD20low prePBs. Supplemental Figure 2. Expression of genes coding for B or PC markers. Data are the Affymetrix signals of expression of genes coding for B or PC markers in MBCs, CD20high B cells, CD20low prePBs, CD20- prePBs, PBs, early PCs, and BMPCs using the same color code as in Figure 6A. Data are the mean value ± SD of gene expression determined in 5 separate experiments. * The mean expression is significantly different from that in CD20high B cells. ** The mean expression is significantly different from that in D4 CD20low prePBs. Supplemental Figure 3. Expression of genes coding for homing molecules. Data are the Affymetrix signals of expression of genes coding for remarkable chemokine receptors or integrins in MBCs, CD20high B cells, CD20low prePBs, CD20- prePBs, PBs, early PCs, and BMPCs using the same color code as in Figure 6A. Data are the mean value ± SD of gene expression determined in 5 separate experiments. * The mean expression is significantly different from that in CD20high B cells. ** The mean expression is significantly different from that in D4 CD20low prePBs. A B cyIgM SI=31SI=83 SI=110 SI=215 CD20- CD38+ CD38 Anti-IgM-FITC CD20- CD20low CD20high cyIgA SI=37SI=111 SI=147 SI=167 CD38- CD38- CD38- CD20 Anti-IgA-FITC cyIgG SI=15SI=30 SI=37 SI=59 Anti-IgG-APC CD20high CD20low CD20- CD20- CD38- CD38- CD38- CD38+ C 300 300 * 70 ** *** * *** 60 250 *** 250 *** * * 50 200 200 * * 40 ** 150 * 150 30 * 100 100 20 cyIgG SI cyIgG cyIgA SI cyIgA cyIgM SI cyIgM 50 50 10 0 0 0 CD20high CD20low CD20- CD20- CD38- CD38- CD38- CD38+ Supplemental Figure 1 2000 2000 900 900 TNFRSF8=CD30 7000 EMP1 CD19 CD20 800 CD22 800 CD24 700 * 6000 * ** 1500 700 700 600 1500 ** 5000 600 600 500 4000 500 500 * 1000 1000 400 * 400 400 3000 * 300 ** 300 * 300 500 2000 200 500 200 200 100 100 1000 * * 100 * * 0 0 0 0 0 ** ** 0 ** 600 3000 500 3500 CD40 AICDA CIITA HLA-DMB 7000 1000 MPEG1 500 2500 3000 CD83 400 6000 800 2500 400 2000 5000 300 2000 600 300 1500 * 4000 200 1500 3000 400 200 1000 * 1000 2000 100 * 500 100 200 ** * 500 * * 1000 0 0 ** 0 * 0 * 0 * * * ** 0 *** ** 800 IL4R 500 TNF LTB CD27 CD38 1500 CD31 4000 2000 600 400 3000 2500 3000 1500 1000 300 400 2000 2000 200 1000 1500 * 500 200 * 1000 * 100 * * 1000 500 * ** ** * * ** 500 0 0 0 ** 0 0 0 TNFRSF17 CD59 700 IL6R * 4000 =BCMA * 600 ** 1600 ** 500 3000 1400 1200 * 400 1000 2000 * 300 *** 800 * 600 200 1000 400 100 200 0 0 0 s s s s s s s l B B B C C C P P P B cel eP eP M B r r ly M gh p - p B i ow h l 20 ear 20 20 D D D C C C Supplemental Figure 2 2000 800 CCR1 1000 CCR6 CCR10 S1PR1=EDG1 1500 800 600 750 * 600 1000 ** 400 * * 500 400 250 200 * * * * 500 200 ** 0 0 0 0 600 2000 CCR7 CXCR5 ITGA4 * CD69 500 500 ** 5000 1500 4000 400 400 3000 300 1000 300 * 2000 200 * 200 * 500 ** 1000 100 100 * * * * 0 ** 0 0 ** 0 150 1000 ITGAX ITGB2 ITGB1 * 4000 KLF2 800 800 ** 100 3000 600 600 2000 400 400 50 * * 1000 ** * 200 200 * 0 0 ** 0 0 s s s s s s s C l B B B C C B cel P P P P P re re y M M B p p l B igh - h low ear 20 20 20 D D D C C C Supplemental Figure 3 Supplemental Table I. CD20-CD38- and CD20highCD38-. Gene list. CD20-CD38- genes CD20highCD38- genes Fold Fold Gene ID Gene Name Change Score(d) Gene ID Gene Name Change Score(d) 205945_at IL6R 18,41 4,44 232739_at SPIB 0,01 -2,68 235275_at BMP8B 15,78 3,16 224402_s_at FCRL4 0,01 -5,48 203914_x_at HPGD 14,66 2,65 226818_at MPEG1 0,01 -2,91 204798_at MYB 10,80 7,09 235385_at MARCH-I 0,01 -3,98 222392_x_at PERP 9,68 3,86 223343_at MS4A7 0,01 -4,75 203373_at SOCS2 9,35 5,81 204959_at MNDA 0,01 -4,11 221790_s_at LDLRAP1 8,87 3,35 266_s_at CD24 0,01 -4,43 201324_at EMP1 8,67 3,79 204249_s_at LMO2 0,01 -14,17 206641_at TNFRSF17 6,86 5,37 219517_at ELL3 0,01 -8,97 201243_s_at ATP1B1 6,67 2,67 228055_at NAPSB 0,02 -9,02 233500_x_at CLEC2D 6,45 3,15 217418_x_at MS4A1 0,02 -14,56 201678_s_at DC12 6,22 3,95 205987_at CD1C 0,03 -7,57 39248_at AQP3 6,08 9,19 231093_at FCRH3 0,03 -5,07 200983_x_at CD59 5,32 2,49 228153_at IBRDC2 0,03 -2,97 206729_at TNFRSF8 5,25 2,61 207339_s_at LTB 0,04 -4,80 204254_s_at VDR 5,20 3,22 208018_s_at HCK 0,05 -5,33 221760_at MAN1A1 5,04 12,00 208820_at PTK2 0,05 -2,79 220306_at FAM46C 5,01 3,56 210279_at GPR18 0,05 -5,82 209457_at DUSP5 4,90 3,08 238009_at SOX5 0,06 -5,17 205885_s_at ITGA4 4,73 2,58 213293_s_at TRIM22 0,06 -7,82 203397_s_at GALNT3 4,61 4,02 219014_at PLAC8 0,06 -4,11 206632_s_at APOBEC3B 4,61 4,79 228617_at BIRC4BP 0,06 -2,80 217127_at CTH 4,59 5,13 206126_at BLR1 0,07 -4,05 CD22 /// 1554242_a_at COCH 4,59 3,11 204581_at MAG 0,07 -4,39 202241_at TRIB1 4,58 9,99 216080_s_at FADS3 0,07 -3,79 224802_at NDFIP2 4,52 2,81 221234_s_at BACH2 0,07 -4,31 209921_at SLC7A11 4,52 3,40 213111_at PIP5K3 0,07 -11,02 201397_at PHGDH 4,52 2,93 225123_at SESN3 0,08 -4,27 203474_at IQGAP2 4,44 5,23 215933_s_at HHEX 0,08 -5,42 200951_s_at CCND2 4,13 8,53 205128_x_at PTGS1 0,08 -2,75 GALNAC4S- 203066_at 6ST 4,09 3,15 218032_at SNN 0,09 -2,97 218018_at PDXK 4,08 2,66 205922_at VNN2 0,09 -2,89 219118_at FKBP11 4,03 2,99 204440_at CD83 0,09 -7,96 204900_x_at SAP30 3,97 4,52 203186_s_at S100A4 0,09 -3,34 202558_s_at STCH 3,97 4,01 221011_s_at LBH 0,10 -4,42 224851_at CDK6 3,96 3,14 204994_at MX2 0,10 -3,19 211464_x_at CASP6 3,92 2,59 206983_at CCR6 0,10 -4,88 200628_s_at WARS 3,87 4,70 200696_s_at GSN 0,10 -2,79 217824_at UBE2J1 3,85 5,86 219836_at ZBED2 0,10 -7,02 225512_at ZBTB38 3,78 7,76 1559263_s_at ZC3H12D 0,10 -6,97 202468_s_at CTNNAL1 3,67 3,44 227458_at PDCD1LG1 0,10 -5,18 218073_s_at TMEM48 3,60 3,70 38521_at MAG 0,10 -7,58 228964_at PRDM1 3,57 4,17 230110_at MCOLN2 0,11 -5,00 209695_at PTP4A3 3,55 3,79 223751_x_at TLR10 0,11 -5,58 203971_at SLC31A1 3,53 3,51 229937_x_at LILRB1 0,11 -4,08 216044_x_at FAM69A 3,50 3,58 226748_at LYSMD2 0,11 -3,05 225520_at MTHFD1L 3,49 9,94 203233_at IL4R 0,11 -3,57 222385_x_at SEC61A1 3,47 2,86 224499_s_at AICDA 0,12 -5,11 201206_s_at RRBP1 3,44 3,22 215127_s_at RBMS1 0,12 -3,48 226771_at ATP8B2 3,41 4,57 1552807_a_at SIGLEC10 0,12 -2,99 SLC16A6 /// 230748_at LOC440459 3,41 3,29 215346_at CD40 0,12 -2,70 211430_s_at IGH 3,41 10,17 201315_x_at IFITM2 0,13 -4,05 205047_s_at ASNS 3,35 7,15 203932_at HLA-DMB 0,13 -7,09 242939_at TFDP1 3,35 3,64 210164_at GZMB 0,13 -2,91 201349_at SLC9A3R1 3,30 4,84 205306_x_at KMO 0,13 -4,92 201200_at CREG1 3,21 3,02 212203_x_at IFITM3 0,13 -2,78 222412_s_at SSR3 3,20 3,57 222891_s_at BCL11A 0,14 -5,39 222532_at SRPRB 3,18 2,42 224406_s_at FCRL5 0,14 -5,22 225368_at HIPK2 3,18 9,16 235401_s_at FCRLM1 0,14 -7,68 200924_s_at SLC3A2 3,18 2,81 221044_s_at TRIM34 0,14 -3,07 201565_s_at ID2 3,17 2,71 227646_at EBF 0,14 -6,06 237625_s_at IGKC 3,16 2,42 202464_s_at PFKFB3 0,14 -7,13 SUB1 /// 221727_at SUB1P1 3,15 5,95 209828_s_at IL16 0,15 -2,71 201160_s_at CSDA 3,12 3,52 218076_s_at ARHGAP17 0,15 -2,68 204142_at ENOSF1 3,11 5,55 205671_s_at HLA-DOB 0,16 -3,29 216449_x_at TRA1 3,10 6,60 223287_s_at FOXP1 0,16 -3,63 201021_s_at DSTN 3,09 3,50 201850_at CAPG 0,16 -3,71 1553530_a_at ITGB1 3,07 2,53 227697_at SOCS3 0,16 -4,16 230352_at PRPS2 3,06 4,92 215565_at DTNB 0,16 -5,78 222231_s_at PRO1855 3,06 11,04 205098_at CCR1 0,17 -4,47 203967_at CDC6 3,05 2,49 224374_s_at EMILIN2 0,17 -2,97 223062_s_at PSAT1 3,03 7,55 204352_at TRAF5 0,17 -5,83 214512_s_at SUB1 2,97 3,20 204446_s_at ALOX5 0,17 -7,65 221253_s_at TXNDC5 2,95 7,06 211962_s_at ZFP36L1 0,17 -7,14 218681_s_at SDF2L1 2,92 2,94 210538_s_at BIRC3 0,17 -6,09 201923_at PRDX4 2,91 4,79 228343_at POU2F2 0,18 -4,02 212110_at SLC39A14 2,91 4,18 222450_at TMEPAI 0,18 -3,68 204695_at CDC25A 2,90 3,40 230252_at GPR92 0,19 -3,66 226982_at ELL2 2,89 5,43 205859_at LY86 0,19 -3,46 224679_at MESDC2 2,88 3,22 232543_x_at ARHGAP9 0,19 -8,11 212501_at CEBPB 2,88 4,02 203281_s_at UBE1L 0,19 -3,53 200895_s_at FKBP4 2,85 3,49 202748_at GBP2 0,20 -3,48 203968_s_at CDC6 2,85 3,93 214022_s_at IFITM1 0,20 -3,21 226750_at LARP2 2,83 3,64 203271_s_at UNC119 0,20 -2,70 201468_s_at NQO1 2,83 2,60 204057_at IRF8 0,20 -6,78 219869_s_at SLC39A8 2,83 2,40 212827_at IGHM 0,20 -11,66 209340_at UAP1 2,83 4,22 212774_at ZNF238 0,21 -7,99 1553954_at ALG14 2,80 3,11 225570_at SLC41A1 0,21 -4,53 202375_at SEC24D 2,77 2,55 223162_s_at LCHN 0,22 -3,31 200617_at KIAA0152 2,76 3,56 238429_at TMEM71 0,22 -4,36 200889_s_at SSR1 2,75 5,39 213572_s_at SERPINB1 0,22 -3,58 204502_at SAMHD1 2,75 3,02 225775_at TSPAN33 0,22 -4,41 204639_at ADA 2,74 2,56 202732_at PKIG 0,23 -3,63 202721_s_at GFPT1 2,73 2,57 228234_at TICAM2 0,23 -5,28 213440_at RAB1A 2,73 2,48 218312_s_at ZNF447 0,23 -2,99 204033_at TRIP13 2,70 6,36 209558_s_at HIP1R 0,23 -4,00 200755_s_at CALU 2,69 2,63 200644_at MARCKSL1 0,23 -2,94 213836_s_at WIPI49 2,69 4,74 207777_s_at SP140 0,23 -9,22 223209_s_at SELS 2,67 4,59 204882_at ARHGAP25 0,23 -4,91 219110_at NOLA1 2,65 4,14 229597_s_at WDFY4 0,23 -3,91 223513_at CENPJ 2,65 2,61 242239_at NSUN6 0,23 -2,80 203200_s_at MTRR 2,65 3,26 209795_at CD69 0,23 -5,02 219806_s_at FN5 2,64 2,47 217763_s_at RAB31 0,23 -3,51 226150_at HTPAP 2,64 4,05 226878_at HLA-DOA 0,24 -4,25 223325_at TXNDC11 2,61 3,56