PRKAB1 Antibody [1F9G6] Cat. No.: 13-300 PRKAB1 Antibody [1F9G6] Specifications HOST SPECIES: Mouse SPECIES REACTIVITY: Human, Monkey, Mouse, Rat IMMUNOGEN: Recombinant protein of human PRKAB1 TESTED APPLICATIONS: ICC, IHC, IP, WB WB: ,1:500 - 1:2000 IHC: ,1:50 - 1:200 APPLICATIONS: ICC: ,1:20 - 1:50 IP: ,1:50 - 1:200 POSITIVE CONTROL: 1) HeLa 2) PC-12 3) SH-SY5Y 4) NCI-H460 5) Jurkat 6) NIH/3T3 PREDICTED MOLECULAR Observed: 38kDa WEIGHT: October 1, 2021 1 https://www.prosci-inc.com/prkab1-antibody-1f9g6-13-300.html Properties PURIFICATION: Affinity purification CLONALITY: Monoclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: PRKAB1 ALTERNATE NAMES: PRKAB1, AMPK beta 1, AMPK subunit beta-1, AMPKb, AMPK, AMPK beta -1 chain, HAMPKb GENE ID: 5564 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non- catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and BACKGROUND: beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 1, 2021 2 https://www.prosci-inc.com/prkab1-antibody-1f9g6-13-300.html.