UCSB Lab-specific Chemical Hygiene Plan Lab-Specific Chemical Hygiene Plan (CHP) General Information & Standard Operating Procedures (SOPs) for the Safinya Laboratories (MRL rooms 1012, 1012B, 1016, 1024, 1032) Chemical Hygiene Officer (CHO): David Vandenberg ([email protected]) KE, Rev. 3/22/19 UCSB Lab-specific Chemical Hygiene Plan This page intentionally left blank 2 KE, Rev. 3/22/19 UCSB Lab-specific Chemical Hygiene Plan Table of Contents Table of Contents ________________________________________________________3 Preface ________________________________________________________________9 Introduction ___________________________________________________________10 Twelve Commandments for Lab Safety ____________________________________11 UCSB Laboratory Worker Responsibilities _________________________________12 General Laboratory Information _________________________________________13 Laboratory Supervisor (PI) ___________________________________________________________ 13 Laboratory Locations (Building /Rooms) ________________________________________________ 13 Laboratory Safety Coordinators (Safety Czars) ____________________________________________ 13 Department Information (MRL) __________________________________________14 Department Safety Representative / Hazard Communication Coordinator _______________________ 14 Location of the Department Safety Bulletin Board _________________________________________ 14 Location of MRL Building Emergency Assembly Point (EAP) _______________________________ 14 Emergency Information _________________________________________________15 Emergency procedures ___________________________________________________15 Evacuation procedures ___________________________________________________15 First-aid kit ____________________________________________________________15 Spill cleanup materials ___________________________________________________15 Laboratory monitors and alarms ____________________________________________16 Fires _________________________________________________________________16 Fire alarm and Evacuation Guidelines ___________________________________________________ 16 Reporting a fire ____________________________________________________________________ 16 Fire Extinguishers __________________________________________________________________ 16 In the Event of an Injury __________________________________________________17 Serious Injuries ____________________________________________________________________ 17 Other Injuries ______________________________________________________________________ 17 Health & Safety References ______________________________________________18 NOTE: Safety Data Sheets (SDS) (formerly known as Material Safety Data Sheets/MSDS): ____ 18 Written Safety Resources & References ______________________________________18 Electronic Safety Resources & References ___________________________________19 Important Phone Numbers and Contact List ________________________________22 Emergency phone numbers ___________________________________________________________ 22 Other important phone numbers _______________________________________________________ 22 Contact information for Lab members and Amanda Strom ___________________________________ 25 Safinya Lab Chores_____________________________________________________26 Earthquake Safety _____________________________________________________26 3 KE, Rev. 3/22/19 UCSB Lab-specific Chemical Hygiene Plan Lab Safety ____________________________________________________________26 Introduction ____________________________________________________________26 Safety Training Requirements _____________________________________________27 Fundamental Lab Safety Rules and Precautions ________________________________28 General __________________________________________________________________________ 28 Lab Equipment ____________________________________________________________________ 29 Electrical Safety ____________________________________________________________________ 29 Gas cylinder handling _______________________________________________________________ 29 Centrifuge Safety ___________________________________________________________________ 30 Pipetting Safety/Ergonomics __________________________________________________________ 30 Chemical Safety ________________________________________________________30 A Humorous View of Chemical Safety OR What to Look Out For ____________________________ 31 Transporting Chemicals ______________________________________________________________ 31 Fume Hood Usage __________________________________________________________________ 31 Identifying Chemical Hazards _____________________________________________32 Chemical safety labels _______________________________________________________________ 32 Communicating Safety and other Lab Issues __________________________________34 Personal Protective Equipment (PPE) _______________________________________34 Safety Glasses and Other Eye Protection ________________________________________________ 34 Clothing requirements _______________________________________________________________ 35 Closed-Toe Footwear _______________________________________________________________ 35 Lab coats _________________________________________________________________________ 35 Communal PPE ____________________________________________________________________ 36 Gloves ___________________________________________________________________________ 37 Glove Selection Chart _______________________________________________________________ 39 The Laboratory Hazard Assessment Tool (“LHAT”) ____________________________41 Main MRL Labs ___________________________________________________________________ 42 Cell Lab __________________________________________________________________________ 43 LOM area _________________________________________________________________________ 43 Fridges and Freezers _____________________________________________________44 Some Best Lab Practices __________________________________________________44 Using Eppendorf-type pipettes: ________________________________________________________ 44 Labeling of Samples, Solutions etc. _________________________________________45 Lab Safety Equipment ____________________________________________________46 Emergency Showers and Eyewash Stations ______________________________________________ 46 Spills and Exposure to Hazardous Chemicals _________________________________46 Emergency procedure _______________________________________________________________ 46 First Aid After Exposure to Hazardous Chemicals _________________________________________ 46 Spill Cleanup ______________________________________________________________________ 47 Disposal of Hazardous Waste ______________________________________________47 Chemical Waste Disposal ____________________________________________________________ 47 Proper Hazardous Waste Segregation ___________________________________________________ 47 Collecting and Storing Hazardous Waste ________________________________________________ 49 Labeling Hazardous Waste ___________________________________________________________ 49 4 KE, Rev. 3/22/19 UCSB Lab-specific Chemical Hygiene Plan Proper Waste Disposal / EH&S Pickup __________________________________________________ 50 Sharps disposal _________________________________________________________50 Glass Disposal _____________________________________________________________________ 50 Disposal of Razor Blades, Needles, etc. _________________________________________________ 50 Completing Work in the Lab – A Checklist _________________________________51 Get Started ________________________________________________________________________ 51 Get Clear _________________________________________________________________________ 51 Stay Connected ____________________________________________________________________ 52 Standard Operating Procedures (SOPs) ____________________________________53 Background: Standard Operating Procedures __________________________________53 SOP: Use of Acrylonitrile ________________________________________________55 Date of last revision to SOP: Aug. 2014 – Kai Ewert _______________________________________ 55 PI approval of SOP: _________________________________________________________________ 55 Scope of SOP ______________________________________________________________________ 55 Compound information ______________________________________________________________ 55 Approval Required _________________________________________________________________ 55 General precautions _________________________________________________________________ 55 Personal Protective Equipment ________________________________________________________ 56 Engineering/Ventilation Controls ______________________________________________________ 56 Handling, Storage, Cleanup, First Aid, and Disposal Requirements ____________________________ 56 Additional Information ______________________________________________________________ 57 LCSS for ACRYLONITRILE _____________________________________________58 Documentation of Training – SOP Use of Acrylonitrile _________________________60 SOP: Use of Benzene ____________________________________________________61 Date of last revision to SOP: Aug. 2014 – Kai Ewert _______________________________________ 61 PI approval of SOP: _________________________________________________________________ 61 Scope of SOP ______________________________________________________________________ 61 Compound information ______________________________________________________________