Tomono et al.: Glycan evolution based on phylogenetic profiling 1 Supplementary Table S1. List of 173 enzymes that are composed of glycosyltransferases and functionally-linked glycan synthetic enzymes UniProt ID Protein Name Categories of Glycan Localization CAZy Class 1 Q8N5D6 Globoside -1,3-N -acetylgalactosaminyltransferase 1 Glycosphingolipid Golgi apparatus GT6 P16442 Histo-blood group ABO system transferase Glycosphingolipid Golgi apparatus GT6 P19526 Galactoside 2--L-fucosyltransferase 1 Glycosphingolipid Golgi apparatus GT11 Q10981 Galactoside 2--L-fucosyltransferase 2 Glycosphingolipid Golgi apparatus GT11 Q00973 -1,4 N -acetylgalactosaminyltransferase 1 Glycosphingolipid Golgi apparatus GT12 Q8NHY0 -1,4 N -acetylgalactosaminyltransferase 2 O -Glycan, N -Glycan, Glycosphingolipid Golgi apparatus GT12 Q09327 -1,4-mannosyl-glycoprotein 4--N -acetylglucosaminyltransferase N -Glycan Golgi apparatus GT17 Q09328 -1,6-mannosylglycoprotein 6--N -acetylglucosaminyltransferase A N -Glycan Golgi apparatus GT18 Q3V5L5 -1,6-mannosylglycoprotein 6--N -acetylglucosaminyltransferase B O -Glycan, N -Glycan Golgi apparatus GT18 Q92186 -2,8-sialyltransferase 8B (SIAT8-B) (ST8SiaII) (STX) N -Glycan Golgi apparatus GT29 O15466 -2,8-sialyltransferase 8E (SIAT8-E) (ST8SiaV) Glycosphingolipid Golgi apparatus GT29 P61647 -2,8-sialyltransferase 8F (SIAT8-F) (ST8SiaVI) O -Glycan Golgi apparatus GT29 Q9NSC7 -N -acetylgalactosaminide -2,6-sialyltransferase 1 (ST6GalNAcI) (SIAT7-A) O -Glycan Golgi apparatus GT29 Q9UJ37 -N -acetylgalactosaminide -2,6-sialyltransferase 2 (ST6GalNAcII) (SThM) (SIAT7-B) O -Glycan Golgi apparatus GT29 Q8NDV1 -N -acetylgalactosaminide -2,6-sialyltransferase 3 (ST6GalNAcIII) (SIAT7-C) Glycosphingolipid Golgi apparatus GT29 Q9BVH7 -N -acetylgalactosaminide -2,6-sialyltransferase 5 (ST6GalNAcV) (SIAT7-E) Glycosphingolipid Golgi apparatus GT29 Q969X2 -N -acetylgalactosaminide -2,6-sialyltransferase 6 (ST6GalNAcVI) (SIAT7-F) Glycosphingolipid Golgi apparatus GT29 Q92185 -N -acetylneuraminide -2,8-sialyltransferase (Ganglioside GD3 synthase) (SIAT8-A) (ST8SiaI) Glycosphingolipid Golgi apparatus GT29 -N -acetyl-neuraminyl-2,3--galactosyl-1,3-N -acetyl-galactosaminide -2,6-sialyltransferase Q9H4F1 Glycosphingolipid Golgi apparatus GT29 (ST6GalNAcIV) (SIAT3-C) (SIAT7-D) P15907 -galactoside -2,6-sialyltransferase 1 (ST6GalI) O -Glycan, N -Glycan Golgi apparatus GT29 Q96JF0 -galactoside -2,6-sialyltransferase 2 (ST6GalII) O -Glycan, N -Glycan Golgi apparatus GT29 Q11203 CMP-N -acetylneuraminate--1,4-galactoside -2,3-sialyltransferase (ST3GalIII) (Sialyltransferase 6) O -Glycan, Glycosphingolipid, Proteoglycan Golgi apparatus GT29 Q11201 CMP-N -acetylneuraminate--galactosamide--2,3-sialyltransferase 1 (ST3GalI) (SIAT4-A) O -Glycan, Glycosphingolipid, Proteoglycan Golgi apparatus GT29 Q16842 CMP-N -acetylneuraminate--galactosamide--2,3-sialyltransferase 2 (ST3GalII) (SIAT4-B) O -Glycan, Glycosphingolipid, Proteoglycan Golgi apparatus GT29 Q11206 CMP-N -acetylneuraminate--galactosamide--2,3-sialyltransferase 4 (ST3GalIV) (STZ) (SIAT4-C) Glycosphingolipid Golgi apparatus GT29 Q92187 CMP-N -acetylneuraminate-poly--2,8-sialyltransferase (SIAT8-D) (ST8SiaIV) N -Glycan Golgi apparatus GT29 Q9UNP4 Lactosylceramide -2,3-sialyltransferase (Ganglioside GM3 synthase) (ST3GalV) (Sialyltransferase 9) Glycosphingolipid Golgi apparatus GT29 O43173 Sia--2,3-Gal--1,4-GlcNAc-R: 2,8-sialyltransferase (SIAT8-C) (ST8SiaIII) N -Glycan, Glycosphingolipid Golgi apparatus GT29 Q9Y274 Type 2 lactosamine -2,3-sialyltransferase (ST3GalVI) (Sialyltransferase 10) Glycosphingolipid Golgi apparatus GT29 Q9UNA3 -1,4-N -acetylglucosaminyltransferase O -Glycan Golgi apparatus GT32 Q9NPC4 Lactosylceramide 4--galactosyltransferase Glycosphingolipid Golgi apparatus GT32 Q8NAT1 Glycosyltransferase-like domain-containing protein 2 O -Glycan Extracellular GT61 O94777 Dolichol phosphate-mannose biosynthesis regulatory protein N -Glycan, GPI anchor Endoplasmic reticulum - Q9P2X0 Dolichol-phosphate mannosyltransferase subunit 3 N -Glycan Endoplasmic reticulum - P0C6T2 Dolichyl-diphosphooligosaccharideprotein glycosyltransferase subunit 4 N -Glycan Endoplasmic reticulum - Q3MUY2 Phosphatidylinositol N -acetylglucosaminyltransferase subunit Y GPI anchor Endoplasmic reticulum - Q8TBF5 Phosphatidylinositol-glycan biosynthesis class X protein GPI anchor Endoplasmic reticulum - Endoplasmic reticulum, Cytoplasm, Q14442 Phosphatidylinositol N -acetylglucosaminyltransferase subunit H GPI anchor - Mitochondria, Nucleus O75072 Fukutin O -Glycan Golgi apparatus+Endoplasmic reticulum - Tomono et al.: Glycan evolution based on phylogenetic profiling 2 Supplementary Table S1. List of 173 enzymes that are composed of glycosyltransferases and functionally-linked glycan synthetic enzymes UniProt ID Protein Name Categories of Glycan Localization CAZy Class 2 Q16880 2-hydroxyacylsphingosine 1--galactosyltransferase Glycosphingolipid - GT1 O -Glycan, N -Glycan, Glycosphingolipid, P15291 -1,4-galactosyltransferase 1 Golgi apparatus GT7 Proteoglycan O60909 -1,4-galactosyltransferase 2 O -Glycan, N -Glycan, Proteoglycan Golgi apparatus GT7 O -Glycan, N -Glycan, Glycosphingolipid, O60512 -1,4-galactosyltransferase 3 Golgi apparatus GT7 Proteoglycan O60513 -1,4-galactosyltransferase 4 Glycosphingolipid, Proteoglycan Golgi apparatus GT7 O43286 -1,4-galactosyltransferase 5 O -Glycan Golgi apparatus GT7 Q9UBX8 -1,4-galactosyltransferase 6 Glycosphingolipid Golgi apparatus GT7 Q9UBV7 -1,4-galactosyltransferase 7 Proteoglycan Golgi apparatus GT7 Q6L9W6 -1,4-N -acetylgalactosaminyltransferase 3 N -Glycan Golgi apparatus GT7 Q8TDX6 Chondroitin sulfate N -acetylgalactosaminyltransferase 1 Proteoglycan Golgi apparatus GT7 Q8N6G5 Chondroitin sulfate N -acetylgalactosaminyltransferase 2 Proteoglycan Golgi apparatus GT7 Q76KP1 N -acetyl--glucosaminyl-glycoprotein 4--N -acetylgalactosaminyltransferase 1 N -Glycan Golgi apparatus GT7 Q86X52 Chondroitin sulfate synthase 1 Proteoglycan Golgi apparatus GT7, GT31 Q8IZ52 Chondroitin sulfate synthase 2 Proteoglycan Golgi apparatus GT7, GT31 Q70JA7 Chondroitin sulfate synthase 3 Proteoglycan Golgi apparatus GT7, GT31 A0PJZ3 Glucoside xylosyltransferase 2 O -Glycan - GT8 Q8NBI6 Xyloside xylosyltransferase 1 O -Glycan Endoplasmic reticulum GT8 Q4G148 Glucoside xylosyltransferase 1 O -Glycan Golgi apparatus GT8 Q6P4F1 -(1,3)-fucosyltransferase 10 N -Glycan Golgi apparatus GT10 P22083 -(1,3)-fucosyltransferase 4 O -Glycan, Glycosphingolipid Golgi apparatus GT10 Q11128 -(1,3)-fucosyltransferase 5 Glycosphingolipid Golgi apparatus GT10 P51993 -(1,3)-fucosyltransferase 6 Glycosphingolipid Golgi apparatus GT10 Q11130 -(1,3)-fucosyltransferase 7 O -Glycan, Glycosphingolipid Golgi apparatus GT10 Q9Y231 -(1,3)-fucosyltransferase 9 O -Glycan, Glycosphingolipid Golgi apparatus GT10 P21217 Galactoside 3(4)-L-fucosyltransferase Glycosphingolipid Golgi apparatus GT10 P26572 -1,3-mannosyl-glycoprotein 2--N -acetylglucosaminyltransferase N -Glycan Golgi apparatus GT13 Q8WZA1 Protein O -linked-mannose -1,2-N -acetylglucosaminyltransferase 1 O -Glycan Golgi apparatus GT13 Q02742 -1,3-galactosyl-O -glycosyl-glycoprotein -1,6-N -acetylglucosaminyltransferase O -Glycan Golgi apparatus GT14 O95395 -1,3-galactosyl-O -glycosyl-glycoprotein -1,6-N -acetylglucosaminyltransferase 3 O -Glycan Golgi apparatus GT14 Q9P109 -1,3-galactosyl-O -glycosyl-glycoprotein -1,6-N -acetylglucosaminyltransferase 4 O -Glycan Golgi apparatus GT14 Q6ZNI0 -1,3-galactosyl-O -glycosyl-glycoprotein -1,6-N -acetylglucosaminyltransferase 7 O -Glycan Golgi apparatus GT14 Q06430 N -acetyllactosaminide -1,6-N -acetylglucosaminyl-transferase, isoform B Glycosphingolipid Golgi apparatus GT14 Q86Y38 Xylosyltransferase 1 Proteoglycan Golgi apparatus+Endoplasmic reticulum GT14 Q9H1B5 Xylosyltransferase 2 Proteoglycan Golgi apparatus+Endoplasmic reticulum GT14 Q10469 -1,6-mannosyl-glycoprotein 2--N -acetylglucosaminyltransferase N -Glycan Golgi apparatus GT16 Q9BYC5 -(1,6)-fucosyltransferase N -Glycan, Proteoglycan Golgi apparatus GT23 Q8NBJ5 Procollagen galactosyltransferase 1 O -Glycan Endoplasmic reticulum GT25 Q8IYK4 Procollagen galactosyltransferase 2 O -Glycan Endoplasmic reticulum GT25 Q7Z4T8 Putative polypeptide N -acetylgalactosaminyltransferase-like protein 5 O -Glycan Golgi apparatus GT27 Q86SF2 N -acetylgalactosaminyltransferase 7 O -Glycan Golgi apparatus GT27, CBM13 Q10472 Polypeptide N -acetylgalactosaminyltransferase 1 O -Glycan Golgi apparatus GT27, CBM13 Q10471 Polypeptide N -acetylgalactosaminyltransferase 2 O -Glycan Golgi apparatus GT27, CBM13 Q14435 Polypeptide N -acetylgalactosaminyltransferase 3 O -Glycan Golgi apparatus GT27, CBM13 Q8N4A0 Polypeptide N -acetylgalactosaminyltransferase 4 O -Glycan Golgi apparatus GT27, CBM13 Q7Z7M9 Polypeptide N -acetylgalactosaminyltransferase 5 O -Glycan Golgi apparatus GT27, CBM13 Q8NCL4 Polypeptide N -acetylgalactosaminyltransferase 6 O -Glycan Golgi apparatus GT27, CBM13 Q9HCQ5 Polypeptide N -acetylgalactosaminyltransferase 9 O -Glycan Golgi apparatus GT27, CBM13 Q86SR1 Polypeptide N -acetylgalactosaminyltransferase 10 O -Glycan Golgi apparatus GT27, CBM13 Q8NCW6 Polypeptide N -acetylgalactosaminyltransferase 11 O -Glycan Golgi apparatus GT27, CBM13 Tomono et al.: Glycan evolution based on phylogenetic profiling 3 Supplementary Table S1. List of 173 enzymes that are composed of glycosyltransferases and functionally-linked glycan synthetic enzymes UniProt ID Protein Name Categories of Glycan Localization CAZy Q8IXK2 Polypeptide N -acetylgalactosaminyltransferase