Seminar Kimia Surakarta, December 8th, 2017 Proceeding of Chemistry Conferences vol. 3 (2018) ISSN 2541-108X i Seminar Kimia Surakarta, December 8th, 2017 Proceeding of Chemistry Conferences vol. 3 (2018) ISSN 2541-108X CONTENTS Cover i Contents ii Welcoming speech iii Organizing committee iv List of article in prosiding v ii Seminar Kimia Surakarta, December 8th, 2017 Proceeding of Chemistry Conferences vol. 3 (2018) ISSN 2541-108X Welcome Speech from Committee and Head of Chemistry Department Sebelas Maret University It is a great pleasure that I could take a part in the special event of Seminar Kimia 2017 held by Departement of Chemistry, Sebelas Maret University. This event is attended by students and purposes to encourage the research and study motivation of the students. Some of the attendance presented a valuable and reviews of the recent research paper in the poster session. The presented article was then compiled and published in the Proceeding of Chemistry Conferences vol 3 (2018). We do hope the meeting and the proceeding will provide a significant contribution to science and technology especially in the Chemistry field to foster a more supportive academic aura. Surakarta, June 2018 Head of Chemistry Department UNS Chairman Dr.Triana Kusumaningsih, M.Si Teguh Endah Saraswati, M.Sc., Ph.D iii Seminar Kimia Surakarta, December 8th, 2017 Proceeding of Chemistry Conferences vol. 3 (2018) ISSN 2541-108X ORGANIZING COMMITTEE OF Lectures and Workshop: In Series of Nanotechnology and Nanomaterial Plasma Science and Technology for Nanomaterial Engineering November 3-4, 2016 Conference Advisory Board: • Dr. Triana Kusumaningsih (Sebelas Maret University, Indonesia) • Dr. Soerya Dewi Marliyana (Sebelas Maret University, Indonesia) Scientific Committee: PUBLICATION, PROCEEDING AND DOCUMENTATION: • Dr. Fitria Rahmawati, Sebelas Maret University • Teguh Endah Saraswati, PhD, Sebelas Maret University Organizing committee: Teguh Endah Saraswati, Ph.D General Chair (Sebelas Maret University) Secretary Fauzan Ibnu P Treasurer, Venue & Facilities Bilqies Musyarofah Secretariat and registration Muhammad Zulkarnain Technical program committee Muhammad Rizki Arwanda Poster program committee Kholifah Afrianti iv Seminar Kimia Surakarta, December 8th, 2017 Proceeding of Chemistry Conferences vol. 3 (2018) ISSN 2541-108X List of published articles in Proceeding of Chemistry Conferences Vol 3 (2018) (https://jurnal.uns.ac.id/pcc/) Microscopy Electron Microscope for Biological Specimen Observation 1-11 Qonita Awliya Hanif*, Carissa Hertiningtyas, Teguh Endah Saraswati Biochemistry Uniporter Transport Ion Calcium in Mitochondria 12-17 Ita Apriana*, Novia Sariningsih, Nuryah Muchlisa Baharuddin Pagalla, Weny Putri Timur Optimizing Photosynthesis Reactions in Plants by Utilizing the Light 18-24 Arina Wahyu Himawati*, Fauzan Ibnu Prihadiyono, Rahis Rahmata Insulin Activity for Carbohydrate Metabolism in Diabetic Mellitus 25-34 M. Rizki Arwanda*, Sarah Rafidah, Uswatun Khasanah Depression Relationship Against Cardiovascular Disease (CVD) and Prevention Using 35-44 Omega-3 Fatty Acids Moh. Ali Khafidin*, Rizki Nilasari, Robiah Al-adawiyah Non-essential Amino Acid as an Inhibitor of Cancer Cell Growth 45-51 Alfian Nur Firdaus*, Nisa Nur Hayati, Rohmatul Awaliya Kreb Cycle and The Effect in Alzheimer Desease 52-58 Bilqies Musyarrofah, Elli Elmatiana, Rinaldi Wahab Lubis* The Function and Alternative DNA Structure 59-70 Abdurrazzaq Ahmad El Yumin, Aisyah Izzatun Nisa’ , Disa Ayudia* Using siRNA as Gen Therapy in HIV/AIDS 71-78 Ardhia Dewi Shavira*, Iin Kistianna, Tri Rohmahwati DNA Damage and Repair Mechanisms in Humans 79-91 Marita Maharani Putri*, Kinkind Raras Heliani, Ayu Setyaningrum The Effect of Acetazolamide and Arginine Hydrochloride on Metabolic Alkalosis 92-100 v Seminar Kimia Surakarta, December 8th, 2017 Proceeding of Chemistry Conferences vol. 3 (2018) ISSN 2541-108X Gracia Lasma Rohana*, Desi Dyah Laksmitasari, Septin Dwi Anggraini Nanoscience Review of Silver Nanoparticles in Medical Fields as Antimicroba Materials 101-107 Era Putri Anandita, Lia Fatawati, Mohammad Rifqi Ihsan, Syahna Febrianastuti*, Yulita Kuswi Wuryaningsih Application of Nanotechnology to Detect Melamine in Milk 108-126 Ajeng Aullia Martina*, Ema Nova Fajariani, Oktaviani Nur Aniza, Tria Hikma Novita, Ucik Refani Kurnia Sari Modification of Carbon Nanotube (CNT) as Removal Heavy Metal Nanotechnology on 127-136 Aqueous Solution Annisa Syam*, Bondan Mutiara Wildam Sunardi, Elsa Ninda Karlinda Putri, Elsanty Nur Afifah, Jessica Primanita R Review of Nanotechnology Applications on Textiles: UV Blocking and Antibacterial 137-142 Annisa Dinan Ghaisani, Faisal Atif Fawazeni, Indah Retnosari, Octaria Priwidya Sari, Yudha Pratama Putra* Nanotechnology on Asphalt Mixture 143-149 Desy Maharani*, Aufara Mahayum, Dielsa Eka Kuswari, Inayatun Farichah, & Umam Hasan Setiawan Nanotechnology Applications for Energy Storage: Batteries and Supercapacitors 150-154 Burhan F. Rahman*, Diah A. Rivani, Nining Rahmawati, dan Rafinda M. Aliestyani Plasma Applications Needle Plasma (Nelma) as an Alternative to Liver Cancer Cell Ablation Cultured In 155-161 Vitro) Nuhaa Faaizatunnisa*, Linda Purnama Sari, Elli Elmatiana, Risqi Nadya Oktaviany Putri, Robiah Al-Adawiyah, Rifqi Ihsan The Electrical Property of Arc Column in Plasma Arc Welding 162-171 Disa Ayudia, Iin Kistianna*, Isma Nur Avifah, Nisa Nur Hayati, Septin Dwi Anggraini, Zyulfi Faiz Application of Plasma Technology as Water Treatment Using the Dielectric Barrier 172-180 Discharge (DBD) Method vi Seminar Kimia Surakarta, December 8th, 2017 Proceeding of Chemistry Conferences vol. 3 (2018) ISSN 2541-108X Nining Rahmawati, Shella Befi Wahyuning, Ardhia Dewi Shavira, Arifti Nur Laily Aqna, Fitri Astuti, Muhammad Zulkarnain*, Sali Artriyani Istiqomah Plasma Sterilization Using Glow Discharge 181-188 Alfian Nur Firdaus, Anita Rinadi, Elyna Wahyu Trisnawati*, Gracia Lasma Rohana, Ria Anisa Rahma, Wahyu Puji Pamungkas Energy and Material Recovery From Waste By Integrated Plasma Gasification/Fuel 189-197 Cell (IPGFC)) Alfiyatul Fithri*, Easy Vicky M, Hilda Alfiani S, Ita Apriana, Rizki Nilasari, Rochmad Eko Cahyono vii Seminar Kimia Surakarta, December 8th, 2017 Proceeding of Chemistry Conferences vol. 3 (2018) ISSN 2541-108X Mikroskop Elektron untuk Observasi Spesimen Biologi (Electron Microscope for Biological Specimen Observation) Qonita Awliya Hanif*, Carissa Hertiningtyas, Teguh Endah Saraswati Program Studi Magister Ilmu Kimia, Pascasarjana, Universitas Sebelas Maret, Jl. Ir. Sutami 36 A, Kentingan-Jebres Surakarta, Jawa Tengah, Indonesia, 57126, (0271) 663375 *Email : [email protected] Abstrak. Mikroskop elektron merupakan instrumen yang secara luas digunakan untuk melihat morfologi spesimen biologi dan material. Namun perlakuan untuk investigasi spesimen biologi tidak sama dengan material anorganik pada umumnya. Hal ini dikarenakan sensitivitas terhadap radiasi, kondisi vakum, adanya kandungan air, dan rendahnya kontras yang dihasilkan dari spesimen biologi. Sifat tersebut menjadi tantangan tersendiri dalam menjaga spesimen biologi agar tidak rusak maupun mengalami perubahan yang signifikan ketika dikarakterisasi dengan mikroskop elektron. Pada tulisan ini diurai mengenai teknik preparasi sebelum karakterisasi, teknik pelapisan dengan cairan ionik, dan prosedur memperoleh multiwarna untuk spesimen biologi dengan menggunakan instrumen berbasis mikroskop elektron. Kata kunci: Mikroskop elektron, spesimen biologi, teknik preparasi, cairan ionik, multiwarna Abstract. Microscope electron is an instrument that widely used to observe the morphology of biological specimens and materials. Nevertheless, the treatment for investigation of biological specimen is different than others inorganic materials in general. This because of the sensitivity towards radiation, vacuum condition, water containing, and low contrast of biological specimen. These properties became a challenge about how to develop technique that suitable to prevent the destruction or collapse of biological specimen. Here we discuss about some preparation techniques before characterization, coating technique with ionic liquid, and procedure to obtain multicolor graphic using microscope electron based instrument. Keywords: Electron Microscope, ionic liquids, biological specimen, multicolor 1. Pendahuluan Mikroskop elektron adalah instrumen yang telah secara luas digunakan dalam mengobservasi material anorganik seperti logam, alloy, komposit maupun organik, seperti tumbuhan, sel, jaringan, virus, atau bakteri. Tidak seperti mikroskop optik pada umumnya, mikroskop ini menggunakan elektron, dalam menghasilkan pencitraan. Elektron dihasilkan dari pistol elektron dengan energi berkisar antara ~500 eV dan 30 keV [1]. Semua mikroskop elektron membutuhkan kondisi vakum baik untuk kepentingan pergerakan elektron maupun meminimalisir hamburan lain dari sampel. Prototipe dari mikroskop elektron pertama kali dibuat oleh Ernst Ruska and Max Knoll pada tahun 1931, yang dapat melihat objek dengan perbesaran hingga seratus kali [2]. Secara umum mikroskop elektron terdiri dari Scanning Electron 1 Seminar Kimia Surakarta, December 8th, 2017 Proceeding of Chemistry Conferences vol. 3 (2018) ISSN 2541-108X Microscope (SEM) dan Transmission Electron Microscope (TEM) ), yang terakhir dikembangkan adalah Scanning Transmission Electron Microscope (STEM) [3]. Tidak lama setelah Ernst Ruska, peraih Nobel Prize tahun 1986, mendemonstrasikan mikroskop elektron [2], Marton mempublikasikan