Recombinant Mouse Integrin alpha x beta 2, CF Datasheet Catalog Number: PR15089CF Product Type: Recombinant Protein Source: Chinese Hamster Ovary cell line, CHO- derived mouse Integrin alpha X beta 2 protein. Description: Integrin alpha X beta 2, also called CD11c/CD18, p150/95 or complement receptor type 4 (CR4), is one of four beta 2 integrins. The non-covalent heterodimer of 150 kDa alpha X/CD11c and 95 kDa beta 2/CD18 integrin subunits is commonly used as a marker for dendritic cells (DC) and classically activated macrophages (M1), but is also expressed on hairy cell leukemias, with lower amounts on other myeloid cells and activated B, NK and some cytotoxic T cells. Inflammation enhances surface expression of alpha X beta 2 in states such as obesity (adipose DC), asthma (lung DC) and hypertriglyceridemia (circulating monocytes), increasing the adhesive capacity of the cells and contributing to pathology. The alpha X vWFA or I‑domain, which contains the adhesion sites, forms the N‑terminal head region with the alpha X beta-propeller and the beta 2 vWFA domain. Molecular Mass: 131 kDa (Integrin alpha X) & 83 kDa (Integrin beta 2) Purity: Greater than 95.0% as determined by: (a) Analysis by SDS-PAGE. Biological Activity: Measured by the ability of the immobilized protein to support adhesion of J45.01 human acute lymphoblastic leukemia T lymphocytes. The ED50 for this effect is 0.2-1.0 μg/mL. Format: Lyophilized from a 0.2 μm filtered solution in PBS. Reconstitution: Reconstitute at 100 μg/mL in PBS. Storage: This product can be stored for up to 1 month at 2°C to 8°C under sterile condition after reconstitution, for up to 3 months at -20°C to -70°C under sterile condition after reconstitution, or for up to 12 months at -20°C to -70°C as supplied. Avoid freeze-thaw cycles. FOR RESEARCH USE ONLY NEUROMICS’ REAGENTS ARE FOR IN VITRO AND CERTAIN NON-HUMAN IN VIVO EXPERIMENTAL USE ONLY AND NOT INTENDED FOR USE IN ANY HUMAN CLINICAL INVESTIGATION, DIAGNOSIS, PROGNOSIS, OR TREATMENT. THE ABOVE ANALYSES ARE MERELY TYPICAL GUIDES. THEY ARE NOT TO BE CONSTRUED AS BEING SPECIFICATIONS. ALL OF THE ABOVE INFORMATION IS, TO THE BEST OF OUR KNOWLEDGE, TRUE AND ACCURATE. HOWEVER, SINCE THE CONDITIONS OF USE ARE BEYOND OUR CONTROL, ALL RECOMMENDATIONS OR SUGGESTIONS ARE MADE WITHOUT GUARANTEE, EXPRESS OR IMPLIED, ON OUR PART. WE DISCLAIM ALL LIABILITY IN CONNECTION WITH THE USE OF THE INFORMATION CONTAINED HEREIN OR OTHERWISE, AND ALL SUCH RSKS ARE ASSUMED BY THE USER. WE FURTHER EXPRESSLY DISCLAIM ALL WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. www.neuromics.com Neuromics 5325 West 74th Street, Suite 8 Edina, MN 55439 phone 866-350-1500 fax 612-677-3976 e-mail [email protected] .