Bio-Plex Manager Software 6.2 User Guide Bio-Plex Manager Software 6.2 User Guide BIO-RAD TECHNICAL SUPPORT The Bio-Rad Technical Support department in the U.S. is open Monday through Friday, 5:00 AM to 5:00PM, Pacific time. Phone: 1-800-424-6723, option 2 Email: [email protected] (U.S./Canada Only) For technical assistance outside the U.S. and Canada, contact your local technical support office or click the Contact Us link at www.bio-rad.com. NOTICE No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage or retrieval system, without permission in writing from Bio-Rad. Bio-Rad reserves the right to modify its products and services at any time. This guide is subject to change without notice. Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors or omissions, or for any damages resulting from the application or use of this information. Bio-Rad and Bio-Plex are trademarks of Bio-Rad Laboratories, Inc. in certain jurisdictions. The Bio-Plex Suspension Array System includes fluorescently labeled microspheres and instrumentation licensed to Bio-Rad Laboratories, Inc. by the Luminex Corporation. All trademarks used herein are the property of their respective owner. Copyright © 2001–2018 by Bio-Rad Laboratories. All rights reserved. Table of Contents Chapter 1. Bio-Plex Suspension Array System Overview . 11 Components . 12 Advantages . 13 For More Information. 14 Bio-Rad Technical Support. 14 Chapter 2. Bio-Plex Manager Software Overview . 15 Software Editions . 15 Software Licenses . 16 Analyzing Luminex Data in Bio-Plex Manager . 16 General Workflow . 17 Quick Guide . 17 Types of Files . 18 Key Software Features . 18 Chapter 3. Software Installation . 19 System Requirements . 19 Required Screen Resolution . 20 Installing the Software. 20 Microsoft.NET. 21 Bioplexdata.mdb File . 21 Hardware Protection Key (HASP Key). 22 Installing the HASP License Manager . 22 Uninstalling Bio-Plex Manager . 25 iii Table of Contents Chapter 4. Starting the System . 27 Starting Bio-Plex Manager . 27 Connecting the Computer to Readers Manufactured after November 2015 . 28 Connecting the Computer to Readers Manufactured before November 2015 . 29 Communication between the Array Reader and Microplate Platform . 30 Disconnecting and Reconnecting . 31 Disconnecting with Sleep Mode. 31 Menu and Toolbars . 32 Status Bar . 32 Quick Guide. 33 Sample Needle Adjustment. 33 Chapter 5. Controlling the System . 37 Bio-Plex MCV Plate IV. 38 Start Up . 39 Optics Warm Up and Shut Down. 41 Calibration . 42 Opening the Calibration Dialog Box. 43 Selecting/Entering Calibration Control Numbers . 43 Setting Up the Calibration . 46 Performing the Calibration . 47 Logging the Calibration . 47 Start Up and Calibrate. 49 Wash Between Plates . 50 Remove Air Bubbles . 51 Unclog . 52 Validation . 53 Validation Kit. 53 Validation Kit Control Number . 53 Setting Up a Validation Run . 54 Control Number Selection . 54 Validation Type Selection . 55 Performing a Validation Run. 56 Validation Results. 56 Validation Log. 57 Platform Heater . 60 Instrument Information . 61 iv Table of Contents Eject/Retract Plate . 61 Additional Instrument Functions . 61 Cancel Operation . 63 Shut Down . 63 Instrument Operations Log . 63 Chapter 6. Preparing Protocols . 65 Protocol Files . 65 Creating/Opening Protocol Files . 65 Saving Protocols . 66 Reducing the File Size of Protocols . 66 Multi-Assay Protocols . 68 Uses for Multi-Assay Protocols . 68 Creating a Multi-Assay Protocol. 69 Changing a Protocol from Single to Multi-Assay . 69 Protocol Window. 70 Step 1. Describe Protocol . 71 Step 2. Select Analytes . 72 Step 3. Format Plate . 80 Step 4. Enter Standards Info . 90 Step 5. Enter Controls Info (Optional) . 108 Step 6. Enter Sample Info (Optional) . 110 Chapter 7. Running Protocols . 111 Run Protocol Window . 112 Bead Count . ..