USOO9228204B2 (12) United States Patent (10) Patent No.: US 9.228,204 B2 Pulst et al. (45) Date of Patent: Jan. 5, 2016 (54) CONSTRUCTS FOR MAKING INDUCED 2010, O184051 A1 7/2010 Hochedlinger et al. PLURPOTENT STEM CELLS 2010/0279.404 A1 11/2010 Yamanaka et al. 2010.0311171 A1 12/2010 Nakanishi et al. (71) Applicant: University of Utah Research Foundation, Salt Lake City, UT (US) FOREIGN PATENT DOCUMENTS EP 2263705 12/2010 (72) Inventors: Stefan M. Pulst, Salt Lake City, UT KR 2009 OO83761 8, 2009 (US); Sharan Paul, Salt Lake City, UT WO WO 2008,151058 12/2008 (US); Warunee Dansithong, Salt Lake WO WO 2009/007852 1, 2009 City, UT (US) WO WO 2009/092042 T 2009 WO WO 2009,093O22 T 2009 WO WO 2009/096614 8, 2009 (73) Assignee: University of Utah Research WO WO 2009/131262 10/2009 Foundation, Salt Lake City, UT (US) WO WO 2009,133971 11, 2009 WO WO 2009,140655 11, 2009 (*) Notice: Subject to any disclaimer, the term of this WO WO 2009, 149233 12/2009 patent is extended or adjusted under 35 WO WO 2009,152485 12/2009 U.S.C. 154(b) by 0 days. WO WO 2010/017562 2, 2010 WO WO 2010/O19569 2, 2010 WO WO 2010/028O19 3, 2010 (21) Appl. No.: 13/975,004 WO WO 2010/036923 4/2010 (22) Filed: Aug. 23, 2013 OTHER PUBLICATIONS (65) Prior Publication Data Takahashi (Cell, 2006, vol. 126:663-676).* US 2014/O 170752 A1 Jun. 19, 2014 Yu (Science, 2007, vol. 318, p. 1917-1920).* Nakagawa (Nat Biotechnol, Jan. 2008, vol. 26: 101-106; published Related U.S. Application Data online Nov. 11, 2007).* Okita (Science, Nov. 7, 2008, vol. 322, p. 949-953).* (63) Continuation-in-part of application No. 13/960,305, Feng (Cell StemCell, Apr. 3, 2009, vol. 4, p. 301-312).* filed on Aug. 6, 2013, now abandoned, which is a Shao (Expert Opin. Biol. Ther. Feb. 2010, vol. 10, No. 1, p. 231 continuation of application No. PCT/US2012/025117, 242).* filed on Feb. 14, 2012. Aoi (Science, Aug. 1, 2008, vol. 321, No. 5889, p. 699-702, available (60) Provisional application No. 61/442,695, filed on Feb. online Feb. 14, 2008).* 14, 2011. 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CI2N 15/86 (2013.01); C12N5/0696 Kaji (Nature, Apr. 9, 2009, vol. 458, p. 771-776).* (2013.01); C12N 2501/602 (2013.01); C12N Gonzalez (PNAS, Jun. 2, 2009, vol. 106, No. 22, p. 8918-8922).* 2501/603 (2013.01); C12N 2501/604 (2013.01); CI2N 2501/606 (2013.01); C12N 2510/00 Paul, Annals of Neurol., 2012, vol. 72, Suppl. 16, pp. S103).* (2013.01); C12N 27.10/10343 (2013.01) Toes (PNAS, Dec. 1997, vol. 94, p. 14660-14665).* (58) Field of Classification Search (Continued) USPC ....................................................... 435/320.1 See application file for complete search history. Primary Examiner — Michael Wilson (56) References Cited (74) Attorney, Agent, or Firm — Thorpe North & Western U.S. PATENT DOCUMENTS LLP 2009/0047263 A1 2/2009 Yamanaka et al. 2009,019 1159 A1 7/2009 Sakurada et al. (57) ABSTRACT 2009,019 1171 A1 T/2009 Ma 2009/0227032 A1 9/2009 Yamanaka et al. 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Lowry, et al.; "The many ways to make an iPS cell'; vol. 26, No. 11, Nov. 1, 2008, Nature Biotechnology. * cited by examiner U.S. Patent Jan. 5, 2016 Sheet 1 of 15 US 9.228,204 B2 psokciv-Adshuttle vector xxi-xxy- exix. : ionoiogo as Recottiratio if yies in Bacteria & s 8.33 t : g:8:Kxxx xxxasy - w8xxx - 08 Wirts Proction G. U.S. Patent Jan. 5, 2016 Sheet 2 of 15 US 9.228,204 B2 pSOKc citasy- wector FG. A s: øbin?spw-woyosd| s: Actin Y Acti: G. 2B U.S. Patent Jan. 5, 2016 Sheet 3 of 15 US 9.228,204 B2 SXC iAxi-Soix FG, 3A inefite of experinetta design Ray RS fircasts sittire Day & Acieao viruses pay 3 Change media every day through ay pays 4-7 k Stem cesis tike E. 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